| Project/Area Number |
15300173
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kansai University |
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Principal Investigator |
MIYATA Takashi Kansai University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (50239414)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥16,500,000 (Direct Cost: ¥16,500,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2003: ¥11,800,000 (Direct Cost: ¥11,800,000)
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| Keywords | gel / stimuli-responsive gel / biomolecular interaction / lectin / antigen-antibody binding / molecular recognition / molecular imprinting / biohybrid |
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| Research Abstract |
In this study, we tried to prepare (a) biomolecule-crosslinked gels and (b) biomolecule-imprinted gels as biohybrid gels that undergo volume changes in response to signal biomolecules :
(a) Biomolecule-crosslinked gels
Reversibly glucose-responsive gels were prepared by using complex formation between lectins and polymers with pendant glucose. Biohybrid gels having antigen-antibody complexes as crosslinking points swelled immediately in the presence of a target antigen and shrank again in its absence. The antigen-responsive gels controlled the drug permeation in response to the antigen concentration. DNA-responsive gels were prepared by introducing duplex DNA at crosslinking points. Swelling behavior of the duplex DNA-crosslinked gels was strongly dependent upon the sequence of DNA. Measurements of crosslinking density of their biohybrid gels revealed that their biomolecule-responsive swelling is due to the dissociation of the biomolecular complexes in the presence of a target biomolecul
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e.
(b) Biomolecule-imprinted gels
Glycoprotein-imprinted gels were prepared by biomolecular imprinting in which lectin and antibody were utilized as ligands for a target tumor-marker glycoprotein. The glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. The glycoprotein-responsive shrinking of the imprinted gel was due to formation of lectin-glycoprotein-antibody complexes that acted as reversible crosslinking points. Furthermore, DNA-imprinted gels having two ligand DNAs shrank in a buffer solution containing a target DNA but swelled a little in the presence of DNAs with different sequences. The responsive shrinking behavior of the bimolecule-imprinted gels is attributed to an increase in their crosslinking density by complex formation between ligands and target biomolecules.
As biomolecule-responsive behavior of biomolecule-crosslinked gels and biomolecule-imprinted gels enables the accurate detection and recognition of target biomolecules, they have many potential applications as smart devices in sensing systems and as molecular diagnostics. Less
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