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Development of the imaging system to evaluate of blood flow distribution and metabolic activity in myocardial microcirculation.

Research Project

Project/Area Number 15300174
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKawasaki Medical School

Principal Investigator

OGASAWARA Yasuo  Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)

Co-Investigator(Kenkyū-buntansha) YADA Toyotaka  Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
NAKAMOTO Hiroshi  Kawasaki Medical School, Medical Engineering, Research Associate, 医学部, 助手 (10299183)
MATSUMOTO Takeshi  Osaka University, Graduate School of Engineering Science, Associate Professor, 大学院・基礎工学研究科, 助教授 (30249560)
KAJIYA Fumihiko  Okayama University, Graduate School of Medical and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70029114)
MOCHIZUKI Seiichi  Kawasaki College of Allied Health Professions, Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60259596)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥12,600,000 (Direct Cost: ¥12,600,000)
Keywordsmicrocirculation / myocardium / metabolic activity / imaging / blood flow dictribution / NADH / mitochondria / oxygen tension
Research Abstract

We constructed the fluorescence microscope system for imaging of myocardial metabolic activity and blood flow distribution. To measure erythrocyte velocities in microcirculation under in-vivo condition with an intravital videomicroscope, the newly software was developed. A line segment was set along the capillaries in time-sequential videotaped images and then a spatiotemporal image was constructed along that segment. The angle of striped pattern in the spatiotemporal image which reflects the erythrocyte velocity, was estimated to compute erythrocyte velocity vector mapping. We have succeeded in quantitatively evaluating the in-vivo microcirculation with our videomicroscope system and by spatiotemporal image analyzing method. Regional myocardial vulnerability to dysoxia is heterogeneous ; however, the dynamics of regional dysoxia and the dependence of regional dysoxia on flow heterogeneity are not well understood, especially at a microcirculatory level. Thus, we evaluated epimyocardial NADH fluorescence during stepwise changes in oxygenation and made a comparison between NADH fluorescence and flow distributions, resolved into 0.1×0.1- to 0.4×0.4-mm^2 regions the sizes comparable to a microcirculatory unit, which perfused by a single precapillary arteriole. In another hypoxia-perfused hearts showing the stable patchy fluorescence pattern, tritium-labeled desmethylimipramine was infused and its deposition distribution, i.e., relative flow distribution within epimyocardial layers was measured by digital radiography. The region of higher NADH fluorescence tended to receive lower flow ; the regional flow-fluorescence correlation was significant. These results suggest that regions vulnerable to dysoxia are also hard to recover from dysoxia and that regional vulnerability to dysoxia is highly relevant to regional flow heterogeneity at a microcirculatory level.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004 2003 Other

All Journal Article (11 results) Patent(Industrial Property Rights) (2 results) Publications (2 results)

  • [Journal Article] Exogenous NO suppresses flow-induced endothelium-derived NO production because of depletion of tetrahydrobiopterin2005

    • Author(s)
      Seiichi Mochizuki
    • Journal Title

      Am J Physiol Heart Circ Physiol 288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 微小循環計測-冠循環、腎循環の可視化・計測-2004

    • Author(s)
      小笠原康夫
    • Journal Title

      計測自動制御学会関西支部平成16年度講習会 講習会テキスト「医療技術における計測と制御」

      Pages: 41-49

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synergistic effects of C-peptide and insulin on coronary flow in early diabetic rats2004

    • Author(s)
      Hiroshi Nakamoto
    • Journal Title

      Metabolism 53・3

      Pages: 335-339

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Pattern differences between distributions of microregional myocardial flows in crystalloid -and blood- perfused rat hearts2004

    • Author(s)
      Takeshi Matsumoto
    • Journal Title

      Am J Physiol Heart Circ Physiol 286

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Beneficial role o by hydroxyfasudil, a specific Rho-kinase inhibitor, on ischemia-reperfusion injury in canine coronary microcirculation in vivo2004

    • Author(s)
      Toyotaka Yada
    • Journal Title

      Microcirculation annual 2004 20

      Pages: 21-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synergistic effects of C-peptide and insulin on coronary flow in early diabetic rats2004

    • Author(s)
      Hiroshi Nakamoto
    • Journal Title

      Metabolism 53(3)

      Pages: 335-339

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Beneficial role of hydroxyfasudil, a specific Rho-kinase inhibitor, on ischemia-reperfusion injury in canine coronary microcirculation in vivo2004

    • Author(s)
      Toyotaka Yada
    • Journal Title

      Microcirculation annual 2004 20

      Pages: 21-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 微小循環計測 -冠循環、腎循環の可視化・計測-2004

    • Author(s)
      小笠原康夫
    • Journal Title

      計測自動制御学会 関西支部 平成16年度講習会 講習会テキスト

      Pages: 41-49

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pattern differences between distributions of microregional myocardial flows in crystalloid -and blood- perfused rat hearts2004

    • Author(s)
      Takashi Matsumoto
    • Journal Title

      Am J Physiol Heart Circ Physiol 286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Analysis of three dimensional architecture of renal microcirculation2003

    • Author(s)
      Yasuo Ogasawara
    • Journal Title

      SPring-8 User Experiment Report 11

      Pages: 129-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysis of three dimensional architecture of renal microcirculation2003

    • Author(s)
      Yasuo Ogasawara
    • Journal Title

      SPring-8 User Experiment Report 129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 高速度撮像装置2004

    • Inventor(s)
      小笠原康夫
    • Industrial Property Rights Holder
      小笠原康夫
    • Industrial Property Number
      2004-055136
    • Filing Date
      2004-01-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 高速度撮像装置2004

    • Inventor(s)
      小笠原 康夫
    • Industrial Property Rights Holder
      小笠原 康夫
    • Industrial Property Number
      2004-055136
    • Filing Date
      2004-01-28
    • Related Report
      2004 Annual Research Report
  • [Publications] 淺野誉久: "ラット交叉潅流モデルを用いたリポソーム型人工赤血球の局所心筋潅流特性の評価-分子トレーサー法を用いた検討-"電子情報通信学会技術研究報告 信学技報. 103・183. 13-16 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yasuo Ogasawara: "Analysis of three dimensional architecture of renal microcirculation"SPring-8 User Experiment Report. 11. 129 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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