Investigation of effects of pest controlling methods including alternative fumigants of methyl bromide on DNA of museum objects.
| Project/Area Number |
15300297
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cultural property science
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| Research Institution | Independent Administrative Institution, National Research Institute for Cultural Properties, Tokyo |
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Principal Investigator |
KIGAWA Rika National Research Institute for Cultural Properties, Tokyo, Department of Conservation Science, Senior Researcher, 保存科学部, 主任研究員 (40261119)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | Cultural properties / DNA analysis / Fumigants / Biodeterioration |
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| Research Abstract |
Treatments for pest eradication on museum objects are often indispensable. But such treatments sometimes cause adverse effects on museum objects. When we look at natural specimens, DNA analysis for taxonomic studies have become increasing common. But some conservators had become concerned about the possibility that some fumigants may adversely affect DNA molecules of natural specimens. Thus we investigated the effects of fumigants which have been used for museum collections on DNA of especially natural specimens in this research. The effects of several treatments for pest eradication on natural specimen DNA were examined using freeze-dried mushroom and freeze-dried muscle. The data clearly showed that the fumigants methyl bromide, methyl bromide/ ethylene oxide mixed gas, ethylene oxide, propylene oxide, and methyl iodide all caused significant degradation of specimen DNA, even with a single fumigation. Subsequent steps at amplification of certain DNA fragments by PCR (polymerase chain reaction) were difficult in these samples. There were more problems in amplifying larger DNA fragments than smaller fragments. The main cause of the decreased efficiency of PCR was thought to be the degradation of template DNA. On the other hand, thermal methods (heating and low temperature), 60 percent carbon dioxide treatment, and fumigation by sulfuryl fluoride seemed scarcely to affect the DNA molecules of the specimens, allowing subsequent PCR to be successfully performed. Direct sequencing of the PCR products was also performed to check the possibility of modifications to the DNA sequences. When the PCR products were amplified to the sufficient amount for the direct sequencing, the DNA sequences were normal in our examination of the few gene regions.
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Report
(5 results)
Research Products
(10 results)
