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Bacterial capturing and biodegradation of fine particles in the ocean

Research Project

Project/Area Number 15310007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionThe University of Tokyo

Principal Investigator

KOGURE Kazuhiro  The University of Tokyo, Ocean Research Institute, Professor, 海洋研究所, 教授 (10161895)

Co-Investigator(Kenkyū-buntansha) MORISAKI Hisao  Ritsumeikan University, College of Science and Engineering, Professor, 理工学部, 教授 (50125671)
CHIURA Hiroshi Xavier  International Christian University, Division of Natural Sciences, Associate Professor, 教養学部, 準教授 (00103698)
WADA Minoru  The University of Tokyo, Ocean Research Institute, Research Associate, 海洋研究所, 助手 (70292860)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥9,000,000 (Direct Cost: ¥9,000,000)
KeywordsMarine bacteria / fine particles / paramagnetic beads / attachment / virus / polymer layer / repulsion energy / 発光細菌 / コロイド粒子 / 原子間力顕微鏡 / エネルギー障壁 / 細菌 / 微細構造 / 鞭毛 / 密度勾配法
Research Abstract

In surface layer of the ocean, there are approximately ten to one hundred million of fine particles of which radius is less than 0.5 micron in one ml of seawater. How those particles are metabolized? How do bacteria interact with those particles? What is the difference between the bacterial interaction with fine particles and large surfaces? This project was conducted to answer these questions. After three years research, the followings have been clarified. First, the concept of "bacterial particle capturing" was proposed after a series of newly developed method using paramagnetic beads. Second, the release of fine particles from marine bacterium, Vibrio fisheri was examined under various physicochemical conditions. It was clarified that the bacterium produce virus like particles containing nucleic acid and may serve as mediator of gene transfer. Third, the importance of surface polymer layers for bacterial interaction with large particles or attachment were investigated and the involvement of repulsion energy was clarified.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (37 results)

All 2006 2005 2004 Other

All Journal Article (28 results) Book (4 results) Publications (5 results)

  • [Journal Article] The surface and endophytic culturable bacterial flora of the maturing seeds of rice plants (Oryza sativa) cultivated in a paddy field.2006

    • Author(s)
      Mano, H, F.Tanaka, A.Watanabe, H.Kaga, S.Okunishi, H.Morisaki
    • Journal Title

      Microbes and Environments 21(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Application of Silkworm Larva assay to determination of peptidoglvcan in natural seawater.2006

    • Author(s)
      Shibata, A, K.Ohwada, K.Tsuchiya, K.Kogure
    • Journal Title

      Journal of Oceanography 62

      Pages: 91-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] An automatic counter of marine bacteria with LED illumination.2006

    • Author(s)
      Nishimura, M, E.Kamiya, K.Kogure
    • Journal Title

      Fisheries Science 72(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Bacterial motility : links to the environment and a driving force for microbial physics.2006

    • Author(s)
      Mitchell, J.G, K.Kogure
    • Journal Title

      FEMS Microbiology Ecology 55

      Pages: 3-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The surface and endophytic culturable bacterial flora of the maturing seeds of rice plants (Oryza sativa) cultivated in a paddy field.2006

    • Author(s)
      Mano, H., F.Tanaka, A.Watanabe, H.Kaga, S.Okunishi, H.Morisaki
    • Journal Title

      Microbes and Environments 21(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Application of Silkworm Larva assay to determination of peptidoglycan in natural seawater.2006

    • Author(s)
      Shibata, A., K.Ohwada, K.Tsuchiya, K.Kogure
    • Journal Title

      Journal of Oceanography 62

      Pages: 91-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] An automatic counter of marine bacteria with LED illumination.2006

    • Author(s)
      Nishimura, M., E.Kamiya, K.Kogure
    • Journal Title

      Fisheries Science 72(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Bacterial motility : links to the environment and a driving force for microbial physics.2006

    • Author(s)
      Mitchell, J.G., K.Kogure
    • Journal Title

      FEMS Microbiology Ecology 55

      Pages: 3-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The proceedings of international symposium on extremophiles and their applications.2006

    • Author(s)
      Sugitate, T., H.X.Chiura
    • Journal Title

      International Society for Extremophiles, Hamburg, Germany 385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction among Marine Organisms2006

    • Author(s)
      K.Kogure (Editor)
    • Journal Title

      Tokai University Press 346

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The surface and endophytic culturable bacterial flora of the maturing seeds of rice plants (Oryza sativa) cultivated in a paddy field.2006

    • Author(s)
      Mano, H., F.Tanaka, A.Watanabe, H.Kaga, S.Okunishi, H.Morisaki
    • Journal Title

      Microbes and Environments 21(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An automatic counter of marine bacteria with LED illumination.2006

    • Author(s)
      Nishimura, M., E.Kamiya, K.Kogure
    • Journal Title

      Fisheries Science 72(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Bacterial Flora of the Biofilm Formed on the Submerged Surface of the Reed Phragmites australis.2005

    • Author(s)
      Yamamoto, M, H.Murai, A.Takeda, S.Okunishi, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 14-24

    • NAID

      110001272890

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characteristics of the Mucus Layer at the Surface of Bluegill (Lepomis macrochirus) and the Bacterial Flora in the Mucus.2005

    • Author(s)
      Hashizume, T, C.Takai, M.Naito, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 69-80

    • NAID

      110001272896

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Effect of the Surface Mucus of Bluegills (Lepomis macrochirus) on Bacterial Activity.2005

    • Author(s)
      Hashizume, T, M.Obayashi, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 97-103

    • NAID

      110001282481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Bacterial Flora of Endophytes in the Maturing Seed of Cultivated Rice (Oryza sativa).2005

    • Author(s)
      Okunishi, S, K.Sako, H.Mano, A.Imamura, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 168-177

    • NAID

      110001840822

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interaction between clay minerals and hydrocarbon-utilizing indigenous microorganisms in high concentration of heavy oil : Implications for bioremediation.2005

    • Author(s)
      Chaerun, S.K, Tazaki, K, Asada, R, Kogure,K.
    • Journal Title

      Clay Minerals 40

      Pages: 105-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Separation of active and inactive fractions from starved culture of Vibrio parahaemolyticus by density dependent cell sorting.2005

    • Author(s)
      Nayak, B.B., E.Kamiya, T., Nishino, M.Wada, M.Nishimura, K.Kogure.
    • Journal Title

      FEMS Microbiology Ecology 51

      Pages: 179-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Bacterial Flora of the Biofilm Formed on the Submerged Surface of the Reed Phragmites australis.2005

    • Author(s)
      Yamamoto, M., H.Murai, A.Takeda, S.Okunishi, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 14-24

    • NAID

      110001272890

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Characteristics of the Mucus Layer at the Surface of Bluegill (Lepomis macrochirus) and the Bacterial Flora in the Mucus.2005

    • Author(s)
      Hashizume, T., C.Takai, M.Naito, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 69-80

    • NAID

      110001272896

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The Effect of the Surface Mucus of Bluegills (Lepomis macrochirus) on Bacterial Activity.2005

    • Author(s)
      Hashizume, T., M.Obayashi, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 97-103

    • NAID

      110001282481

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Bacterial Flora of Endophytes in the Maturing Seed of Cultivated Rice (Oryza sativa).2005

    • Author(s)
      Okunishi, S., K.Sako, H.Mano, A.Imamura, H.Morisaki
    • Journal Title

      Microbes and Environments 20

      Pages: 168-177

    • NAID

      110001840822

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Interaction between clay minerals and hydrocarbon-utilizing indigenous microorganisms in high concentration of heavy oil : Implications for bioremediation.2005

    • Author(s)
      Chaerun, S.K., Tazaki, K., Asada, R., Kogure, K.
    • Journal Title

      Clay Minerals 40

      Pages: 105-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Separation of active and inactive fractioins from starved culture of Vibrio parahaemolyticus by density dependent cell sorting.2005

    • Author(s)
      Nayak, B.B., E.Kamiya, T., Nishino, M.Wada, M.Nishimura, K.Kogure
    • Journal Title

      FEMS Microbiology Ecology 51

      Pages: 179-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Proceedings of 7^<th> Multinational Congress on Microscopy.2005

    • Author(s)
      B.Velimirov, H.X.Chiura, K.Kogure
    • Journal Title

      Slovene Society for Microbiology, Ljubljana, Slovenia 418

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and Characterisation of Broad-Host Range Gene Transporter Particles from Geo-Thermal Vent of Toyoha Mine.2004

    • Author(s)
      Chiura, Hiroshi Xavier, Masataka Umitsu
    • Journal Title

      Microbes and Environments 19

      Pages: 20-30

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 生態系における広宿主域遺伝子伝達粒子の意義2004

    • Author(s)
      千浦 博
    • Journal Title

      Viva Origino 23

      Pages: 118-137

    • NAID

      110003723337

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel Broad-host Range Gene Transfer Particles in Nature2004

    • Author(s)
      Chiura H.X.
    • Journal Title

      Microbes and Environments 19

      Pages: 249-264

    • Related Report
      2004 Annual Research Report
  • [Book] The proceedings of international symposium on extremophiles and their applications.2006

    • Author(s)
      Sugitate, T, H.X.Chiura
    • Total Pages
      385
    • Publisher
      International Society for Extremophiles, Hamburg, Germany
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 海洋生物の連鎖2006

    • Author(s)
      木暮一啓
    • Total Pages
      346
    • Publisher
      東海大学出版会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Book] The proceedings of international symposium on extremophiles and their applications.2006

    • Author(s)
      Sugitate, T., H.X.Chiura
    • Total Pages
      385
    • Publisher
      International Society for Extremophiles, Hamburg, Germany
    • Related Report
      2005 Annual Research Report
  • [Book] Proceedings of 7^<th> Multinational Congress on Microscopy.2005

    • Author(s)
      B.Velimirov, H.X.Chiura, K.Kogure
    • Total Pages
      418
    • Publisher
      Slovene Society for Microbiology, Ljubljana, Slovenia,
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Publications] 千浦博: "細菌致死要因としてのウイルス -Lysogenic Virus-"海洋. 号外No.33. 137-145 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okuda S, Igarashi R, Kusui Y, Kasahara Y, Morisaki H.: "Electrophoretic mobility of Bacillus subtilis knockout mutants with and without flagella."Journal of Bacteriology. 185・13. 3711-3717 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 木暮一啓: "AFMの海洋細菌への応用"AFMの海洋細菌への応用. 38. 83-85 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishino T, Ikemoto E, Kogure K.: "Application of atomic force microscopy toobservation of marine bacterial"Journal of Oceanography. 60. 219-225 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishino, T., B.B.Nayak, K.Kogure: "Density dependent sorting of physiologically different cells of Vibrio parahaemolyticus."Applied and Environmental Microbiology. 69. 3569-3572 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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