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The study on genomic instability initiated by radiation signature using the chromosome transfer techniue

Research Project

Project/Area Number 15310040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka Prefecture University (2004)
Nagasaki University (2003)

Principal Investigator

KODAMA Seiji  Osaka Prefecture University, Research Institute of Advanced Science and Technology, Professor, 先端科学研究所, 教授 (00195744)

Co-Investigator(Kenkyū-buntansha) SHIRAISHI Kazunori  Osaka Prefecture University, Research Institute of Advanced Science and Technology, Research Associate, 先端科学研究所, 助手 (40347513)
鈴木 啓司  長崎大学, 大学院・医歯薬学総合研究科, 助手 (00196809)
渡邉 正己  長崎大学, 大学院・医歯薬学総合研究科, 教授 (20111768)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsGenomic instability / Carcinogenesis / Ionizing radiation / Chromosome transfer / Chromosome aberration / Telomere / テロメア不安定化 / 遅延性染色体異常
Research Abstract

To know the mechanism for genomic instability induced by ionizing radiation, we studied chromosome instability induced by indirect action of radiation using microcell-mediated chromosome transfer. Mouse A9 cells containing a human chromosome 4 or 11 were irradiated with X-rays (4,6,15 Gy), and the irradiated human chromosome was transferred into unirradiated mouse m5S cells by microcell fusion. The stability of the transferred human chromosome was examined by fluorescent in situ hybridization (FISH). The results indicated that the irradiated chromosome per se was unstable compared to the unirradiated chromosome in some microcell hybrids. In addition, an unirradiated human was transferred into 4 Gy-irradiated mouse m5S cells by microcell fusion. The results showed that the unirradiated human chromosome was unstable, interacting with the irradiated mouse chromosomes in some microcell hybrids. These results suggest that an irradiated chromosome has unstable nature that is transmissible over many cell divisions. Moreover, chromosome analysis using telomere FISH indicated that telomere fusions mediated by telomere dysfunction was involved in the instability of the irradiated chromosome. Therefore, the present study indicates the significance of indirect action of radiation in the induction of chromosome instability.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (29 results)

All 2005 2004 2003 Other

All Journal Article (25 results) Publications (4 results)

  • [Journal Article] Induction of micronuclei in CHO cells by bleomycin but not by X-irradiation is decreased by treatment with HMG-CoA reductase inhibitors2005

    • Author(s)
      Iwashita, J., et al.
    • Journal Title

      Journal of Radiation Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Induction of micronuclei in CHO cells by bleomycin but not by X-irradiation is decreased by treatment with HMG-CoA reductase inhibitors.2005

    • Author(s)
      Iwashita, J.
    • Journal Title

      J.Radiat.Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] X-ray-induced telomeric instability in Atm-deficient mouse cells.2004

    • Author(s)
      Undarmaa, B., et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 315

      Pages: 51-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Delayed induction of telomere instability in normal human fibroblast cells by ionizing radiation.2004

    • Author(s)
      Ojima, M., et al.
    • Journal Title

      Journal of Radiation Research 45

      Pages: 105-110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation.2004

    • Author(s)
      Tominaga, T., et al.
    • Journal Title

      Journal of Radiation Research 45

      Pages: 181-188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Stabilization of alanine substituted p53 protein at Ser15, Thr18, and Ser20 in response to ionizing radiation.2004

    • Author(s)
      Yamauchi, M., et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 323

      Pages: 906-911

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Evidence for induction of DNA double strand breaks in the bystander response to targeted soft X-rays in CHO cells.2004

    • Author(s)
      Kashino, G., et al.
    • Journal Title

      Mutation Research 556

      Pages: 209-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] X-ray-induced telomeric instability in Atm-deficient mouse cells.2004

    • Author(s)
      Undarmaa, B.
    • Journal Title

      Biochem.Biophys.Res.Comm. 315

      Pages: 51-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Delayed induction of telomere instability in normal human fibroblast cells by ionizing radiation.2004

    • Author(s)
      Ojima, M.
    • Journal Title

      J.Radiat.Res. 45

      Pages: 105-110

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of reactive oxygen species(ROS) in the induction of genetic instability by radiation.2004

    • Author(s)
      Tominaga, T.
    • Journal Title

      J.Radiat.Res. 45

      Pages: 181-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Stabilization of alanine substituted p53 protein and Ser2O in response to ionizing radiation.2004

    • Author(s)
      Yamauchi, M.
    • Journal Title

      Biochem.Biophys.Res.Comm. 323

      Pages: 906-911

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evidence for induction of DNA double strand breaks in the bystander response to targeted soft X-rays in CHO cells.2004

    • Author(s)
      Kashino, G.
    • Journal Title

      Mutation Res. 556

      Pages: 209-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of telomere dysfunction in the induction of genomic instability by radiation in scid mouse cells.2003

    • Author(s)
      Urushibara, A., et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 313

      Pages: 1034-1040

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Gap junctional intercellular communication and cellular response to heat stress.2003

    • Author(s)
      Hamada, N., et al.
    • Journal Title

      Carcinogenesis 24

      Pages: 1723-1728

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Delayed reactivation of p53 in the progeny of cells surviving ionizing radiation.2003

    • Author(s)
      Suzuki, K., et al.
    • Journal Title

      Cancer Research 63

      Pages: 936-941

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Long-lived, mutagenic radicals induced in mammalian cells by ionizing radiation are mainly localized to proteins.2003

    • Author(s)
      Kumagai, J., et al.
    • Journal Title

      Radiation Research 160

      Pages: 95-102

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Relief of oxidative stress by ascorbic acid delays cellular senescence of normal human and Werner syndrome fibroblast cells.2003

    • Author(s)
      Kashino, G., et al.
    • Journal Title

      Free Radical Biology & Medicine 35

      Pages: 438-443

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Radiation-induced DNA damage and delayed induced genomic instability.2003

    • Author(s)
      Suzuki, K., et al.
    • Journal Title

      Oncogene 22

      Pages: 6988-6993

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Involvement of telomere dysfunction in the induction of genomic instability by radiation in scid mouse cells.2003

    • Author(s)
      Urushibara, A.
    • Journal Title

      Biochem.Biophys.Res.Comm. 313

      Pages: 1034-1040

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Gap junctional intercellular communication and cellular response to heat stress.2003

    • Author(s)
      Hamada, N.
    • Journal Title

      Carcinogenesis 24

      Pages: 1723-1728

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Delayed reactivation of p53 in the progeny of cells surviving ionizing radiation.2003

    • Author(s)
      Suzuki, K
    • Journal Title

      Cancer Res. 63

      Pages: 936-941

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Long-lived, mutagenic radicals induced in mammalian cells by ionizing radiation are mainly localized to proteins.2003

    • Author(s)
      Kumagai, J.
    • Journal Title

      Radiat.Res. 160

      Pages: 95-102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Relief of oxidative stress by ascorbic acid delays cellular senescence of normal human and Werner syndrome fibroblast cells.2003

    • Author(s)
      Kashino, G.
    • Journal Title

      Free Radical Biol.Med. 35

      Pages: 438-443

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Radiation-induced DNA damage and delayed induced genomic instability.2003

    • Author(s)
      Suzuki, K.
    • Journal Title

      Oncogene 22

      Pages: 6988-6993

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Long-lived, mutagenic radicals induced in mammalian cells by ionizing radiation are mainly localized to proteins.2003

    • Author(s)
      Kumagai, J., et al.
    • Journal Title

      Radiation Research. 160

      Pages: 95-102

    • Related Report
      2004 Annual Research Report
  • [Publications] A.Urushibara et al.: "Involvement of telomere dysfunction in the induction of genomic instability by radiation in scid mouse cells"Biochem.Biophys.Res.Comm.. 313. 1034-1040 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] B.Undarmaa et al.: "X-ray-induced telomeric instability in Atm-deficient mouse cells"Biochem.Biophys.Res.Comm.. 315. 51-58 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Tominaga et al.: "Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation"J.Radiat.Res.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Ojima et al.: "Delayed induction of telomere instability in normal human fibroblast cells by ionizing radiation"J.Radiat.Res.. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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