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Detoxification Process Development for Hydrophobic Substances in Aqueous Environment Using Chemically Modified Enzyimes

Research Project

Project/Area Number 15310048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionTokyo Institute of Technology

Principal Investigator

KAJIUCHI Toshio  Tokyo Inst. Tech., Grad. School of Science and Engineering, Professor, 大学院・理工学研究科, 教授 (30016555)

Co-Investigator(Kenkyū-buntansha) SHIN-YA Yoshitsune  Tokyo Inst Tech., Grad. School of Science and Eng., Assistant Professor, 大学院・理工学研究科, 助手 (20313348)
HONG Kyung Jing  Tokyo Inst. Tech., Grad. School of Science and Eng., Assistant Professor, 大学院・理工学研究科, 助手 (40323844)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥10,900,000 (Direct Cost: ¥10,900,000)
KeywordsEnzymatic Reaction / Chemical Modification / Amphiphilic Polymers / Phenol / Environmental Detoxification / Self-Assemble / Laccase / 修飾酵素 / フェノール系化合物 / 環境汚染物質 / 重合無害化 / 除去 / ミセル
Research Abstract

This study is mainly related to the characterization of a modified laccase from the Trametes species for use in the oxidative removal of phenolics in aqueous systems. An amphiphilic copolymer, polyalkyleneoxide-co-maleic anhydride was used as a modifier. The modified laccase displayed higher catalytic activity along with improved thermal stability. The modification resulted in a 20- to 10-fold increase in thermal stability at 25 and 40℃, respectively. The kinetic parameters of both the native and the modified laccases were determined by using 2,6-dimethoxyphenol as a substrate. The modified laccase was applied to remove a range of phenolic compounds including chlorophenols, dichiorophenols, trichlorophenols, xylenol, and cresol, which usually behave as pollutants of natural environments. The modified laccase exhibited a remarkably improved efficiency over that of the native laccase in terms of removing various phenolic substances. These results revealed that the enzymatic oxidation by the modified laccases is suitable for the efficient removal of phenolics in aqueous systems.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (7 results)

All 2005 2004

All Journal Article (7 results)

  • [Journal Article] Efficacy of amphiphile-modified laccase in enzymatic oxidation and removal phenolics in aqueous solution2005

    • Author(s)
      Yoshitsune SHIN-YA, HN AYE, KJ HONG, Toshio KAJIUCHI
    • Journal Title

      Enzyme and Microbial Technology 36

      Pages: 147-152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chemical modification of chicken egg white proteins with polyethyleneoxide- maleic anhydride copolymer and their partitioning in aqueous two-phase systems2005

    • Author(s)
      Yoshitsune Shin-ya, JW Park, KJ Hong, K Fujiwara, ToshioKajiuchi1
    • Journal Title

      Journal of Chemical Technology and Biotechnology 80

      Pages: 338-344

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Efficacy of amphiphile-modified laccase in enzymatic oxidation and removal of phenolics in aqueous solution2005

    • Author(s)
      Yoshitsune SHIN-YA, HN AYE, KJ HONG, Toshio KAJIUCHI
    • Journal Title

      Enzyme and Microbial Technology 36

      Pages: 147-152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chemical modification of chicken egg white proteins with polyethyleneoxide- maleic anhydride copolymer and their partitioning in aqueous two-phase systems2005

    • Author(s)
      Yoshitsune Shin-ya, JW Park, KJ Hong, K Fujiwara, Toshio Kajiuchi
    • Journal Title

      Journal of Chemical Technology and Biotechnology 80

      Pages: 338-344

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Efficacy of amphiphile-modified laccase in enzymatic oxidation and removal of phenolics in aqueous solution2005

    • Author(s)
      Yoshitsune Shin-ya et al.
    • Journal Title

      Enzyme and Microbial Technology 36

      Pages: 147-152

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Enzymatic Oxidation of Phenolic Pollutants Using PAOMA-Modified Laccases in Aqueous Systems2004

    • Author(s)
      Yoshitsune SHIN-YA, HN AYE, KJ HONG, K FUJIWARA, Toshio KAJIUCHI
    • Journal Title

      APPChE 2004 Preprint

      Pages: 262-262

    • NAID

      130005052405

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] エマルジョン系における酵素的バイオディーゼル合成2004

    • Author(s)
      大川裕司, 洪京珍, 新屋義経, 梶内俊夫
    • Journal Title

      化学工学会 第69回年会講演要旨集

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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