Detoxification Process Development for Hydrophobic Substances in Aqueous Environment Using Chemically Modified Enzyimes
| Project/Area Number |
15310048
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KAJIUCHI Toshio Tokyo Inst. Tech., Grad. School of Science and Engineering, Professor, 大学院・理工学研究科, 教授 (30016555)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN-YA Yoshitsune Tokyo Inst Tech., Grad. School of Science and Eng., Assistant Professor, 大学院・理工学研究科, 助手 (20313348)
HONG Kyung Jing Tokyo Inst. Tech., Grad. School of Science and Eng., Assistant Professor, 大学院・理工学研究科, 助手 (40323844)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥10,900,000 (Direct Cost: ¥10,900,000)
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| Keywords | Enzymatic Reaction / Chemical Modification / Amphiphilic Polymers / Phenol / Environmental Detoxification / Self-Assemble / Laccase / 修飾酵素 / フェノール系化合物 / 環境汚染物質 / 重合無害化 / 除去 / ミセル |
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| Research Abstract |
This study is mainly related to the characterization of a modified laccase from the Trametes species for use in the oxidative removal of phenolics in aqueous systems. An amphiphilic copolymer, polyalkyleneoxide-co-maleic anhydride was used as a modifier. The modified laccase displayed higher catalytic activity along with improved thermal stability. The modification resulted in a 20- to 10-fold increase in thermal stability at 25 and 40℃, respectively. The kinetic parameters of both the native and the modified laccases were determined by using 2,6-dimethoxyphenol as a substrate. The modified laccase was applied to remove a range of phenolic compounds including chlorophenols, dichiorophenols, trichlorophenols, xylenol, and cresol, which usually behave as pollutants of natural environments. The modified laccase exhibited a remarkably improved efficiency over that of the native laccase in terms of removing various phenolic substances. These results revealed that the enzymatic oxidation by the modified laccases is suitable for the efficient removal of phenolics in aqueous systems.
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Report
(3 results)
Research Products
(7 results)
