Development of bioremediation and biorefinery process for rare metal using metal-oxoanion reducing and accumulating bacteria

• Project/Area Number: 15310056
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Osaka University
• Principal Investigator: IKE Michihiko Osaka University, Gradate School of Engineering, Professor, 大学院・工学研究科, 教授 (40222856)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Mitsuo Osaka University, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (40220347) ; SEI Kazunari Osaka University, Graduate School of Engineering, Assistant Professor, 大学院・工学研究科, 助手 (80324177) ; 藤田 正憲 大阪大学, 大学院・工学研究科, 教授 (70029289)
• Project Period (FY): 2003 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2004: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2003: ¥6,100,000 (Direct Cost: ¥6,100,000)
• Keywords: rare metal / wastewater treatment / soil purification / selenium / arsenicum / dissimilatory reduction / Bacillus sp.SF-1 / 異化還元作用 / Bacillus sp. SF-1

Research Abstract

For the prevention of environmental pollution by and exhaustion of rare metals which are increasingly consumed in high-tech industries, novel rare metal reducing and accumulating bacteria were screened and applied to rare metal removal/recovery processes.
In the first year, selenate containing wastewater treatment reactor was developed utilizing Bacillus sp.SF-1 which can reduce selenate to elemental selenium via selenate. Model wastewater containing 1-2 mM selenate could be treated with less than 1 day of the hydraulic retention time, and elemental selenium was completely removed and recovered when combining with simple coagulation sedimentation or ultrafiltration.
In the second year, arsenate reduction ability of Bacillus sp.SF-1 was characterized in detail and the feasibility of the bioremediation process for arsenate contaminated soil utilizing Bacillus sp.SF-1 was evaluated. Bacillus sp.SF-1 could utilize various carbon sources and did not require stringent anaerobic condition for s elenate reduction. These characteristics were remarkable benefit for the actual bioremediation process. In addition, Bacillus sp.SF-1 could reduce arsenate even selenate and/or nitrate were coexisting. Thus, it was implied that Bacillus sp.SF-1 has separate reductases which are individually induced by arsenate, selenate and nitrate.
In the final year, lab-scale slurry phase batch reactor experiment was performed. It was confirmed that both high-inoculum concentration of Bacillus sp.SF-1 and agitation were not needed. Therefore, continuous soil packed column reactor, which allows low cost treatment, was investigated to treat model arsenate contaminated soil. This reactor could purify the model arsenate contaminated soil (300 mg-As/kg-soil) to below a soil content standard of arsenate (150 mg-As/kg-soil) with hydraulic retention time of inoculum culture fluid at 1 day when combined with washing by phosphate buffer.
These results indicate the feasibility of the development of arsenate removal/recovery process utilizing metal-oxoanion reducing/accumulating bacteria.

Research Products

• [Journal Article] Reduction kinetics of As(V) to As(III) by a dissimilatory arsenate-reducing bacterium, Bacillus sp.SF-1. (2006)
  • Author(s): Soda, O.S., Yamamura, S., Zhou, H., Ike, M., Fujita,M.
  • Journal Title: Biotechnology and Bioengineering Vol.93(No.4) Pages: 812-815
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reduction of As(V) to As(III) by a dissimilatory arsenate-reducing bacterium, Bacillus sp.SF-1. (2006)
  • Author(s): Soda, O.S., Yamamura, S., Zhou, H., Ike, M., Fujita, M.
  • Journal Title: Biotechnology and Bioengineering 93-4 Pages: 812-815
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reduction kinetics of As(V) to As(III) by a dissimilatory arsenate-reducing bacterium, Bacillus sp.SF-1. (2006)
  • Author(s): Soda, O.S., Yamamura, S., Zhou, H., Ike, M., Fujita, M.
  • Journal Title: Biotechnology and Bioengineering Vol.93, No.4 Pages: 812-815
• [Journal Article] Characterizing kinetics of transport and transformation of selenium in water-sediment microcosm free from selenium contamination using a simple mathematical model. (2005)
  • Author(s): Fujita, M., Ike, M., Hashimoto, R., Nakagawa, T., Yamaguchi, K., Soda,O.S.
  • Journal Title: Chemosphere Vol.58(No.6) Pages: 705-714
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 金属酸化物の還元作用を利用したレアメタルの除去・無毒化 (2005)
  • Author(s): 池道彦
  • Journal Title: 水環境学会誌 Vol.28(No.2) Pages: 63-67
  • NAID: 10014378657
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterizing kinetics of transport and transformation of selenium in water-sediment microcosm free from selenium contamination using a simple mathematical model. (2005)
  • Author(s): Fujita, M., Ike, M., Hashimoto, R., Nakagawa, T., Yamaguchi, K., Soda, O.S.
  • Journal Title: Chemosphere 58-6 Pages: 705-714
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Removal and/or detoxification of rare metals utilizing microbial reduction of metal-oxoanions. (2005)
  • Author(s): Ike, M.
  • Journal Title: Journal of Japan Society on Water Environment 28-2 Pages: 63-67
  • NAID: 10014378657
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kinetics of transport and transformation of selenium discharged into water-sediment system free from selenium contamination. (2005)
  • Author(s): Ike, M., Soda, O.S., Hashimoto, R., Kashiwa, M, Sei, K., Fujita, M.
  • Journal Title: Abstracts of SETAC Europe 15^<th> Annual Meeting Pages: 134-134
• [Journal Article] Arsenic extraction from contaminated soil using microbial arsenate reduction as a novel bioremediation technique. (2005)
  • Author(s): Yamamura, S., Yamamoto, N., Sei, K., Ike, M., Fujita, M.
  • Journal Title: Proceedings of IWA Conference on Sustainable Development and Production for Chemical Industries with the Environment. (CD-ROM)
• [Journal Article] Bacillus sp.SF-1のセレン酸還元関連遺伝子の単離 (2005)
  • Author(s): 黒田真史, 山下光雄, 池道彦
  • Journal Title: 平成17年度日本生物工学会大会講演要旨集 Pages: 68-68
• [Journal Article] 自然土壌環境中のヒ素可動化ポテンシャル (2005)
  • Author(s): 山本規雄, 清和成, 池道彦, 藤田正憲
  • Journal Title: 平成17年度日本生物工学会大会講演要旨集 Pages: 139-139
• [Journal Article] Characterizing kinetics of transport and transformation of selenium in water-sediment microcosm free from selenium contamination using a simple mathematical model. (2005)
  • Author(s): Fujita, M., Ike, M., Hashimoto, R., Nakagawa, T., Yamaguchi, K., Soda, O.S.
  • Journal Title: Chemosphere Vol.58(No.6) Pages: 705-714
• [Journal Article] 金属酸化物の還元作用を利用したレアメタルの除去・無毒化 (2005)
  • Author(s): 池 道彦
  • Journal Title: 水環境学会誌 Vol.28(No.2) Pages: 63-67
  • NAID: 10014378657
• [Journal Article] Bacillus SF-1株による金属酸化物還元作用とそのセレン排水処理およびヒ素汚染土壌浄化への応用 (2004)
  • Author(s): 池道彦, 藤田正憲
  • Journal Title: 水 Vol.46-11 Pages: 18-22
  • NAID: 40006351060
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of arsenate-, selenate- and nitrate-reducing activities in Bacillus sp.SF-1 (2004)
  • Author(s): Yamamura, S., Terashi, S., Ike, M., Yamashita, M., Fujita,M.
  • Journal Title: Japanese Journal of Water Treatment Biology Vol.40(No.4) Pages: 161-168
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Application of metal oxide reduction ability of Bacillus SF-1 for treatment of selenate containing wastewater and bioremediation of arsenate contaminated soil. (2004)
  • Author(s): Ike, M., Fujita, M.
  • Journal Title: Water 46-11 Pages: 18-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of arsenate-, selenate- and nitrate-reducing activities in Bacillus sp.SF-1. (2004)
  • Author(s): Yamamura, S., Terashi, S., Ike, M., Yamashita, M., Fujita, M.
  • Journal Title: Japanese Journal of Water Treatment Biology 40-4 Pages: 161-168
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bacillus sp.SF-1株による金属酸化物還元作用とそのセレン排水処理およびヒ素汚染土壌浄化への応用 (2004)
  • Author(s): 池道彦, 藤田正憲
  • Journal Title: 水 Vol.46-11 Pages: 18-22
• [Journal Article] Characterization of arsenate-,selenate- and nitrate-reducing activities in Bacillus sp.SF-1 (2004)
  • Author(s): Yamamura, S., Terashi, S., Ike, M., Yamashita, M., Fujita, M.
  • Journal Title: Japanese Journal of Water Treatment Biology Vol.40(No.4) Pages: 161-168
• [Journal Article] Dissimilatory arsenate reduction by a facultative anaerobe, Bacillus sp. strain SF-1. (2003)
  • Author(s): Yamamura, S., Ike, M., Fujita, M.
  • Journal Title: Journal of Bioscience and Bioengineering Vol.96(No.5) Pages: 454-460
  • NAID: 210000007441
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dissimilatory arsenate reduction by a facultative anaerobe, Bacillus sp.Strain SF-1. (2003)
  • Author(s): Yamamura, S., Ike, M., Fujita, M.
  • Journal Title: Journal of Bioscience and Bioengineering 96-5 Pages: 454-460
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamamura S, Ike M, Fujita M: "Dissimilatory arsenate reduction by a facultative anaerobe, Bacillus sp. strain SF-1"Journal of Bioscience and Bioengineering. Vol.95(No.5). 454-460 (2003)