• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of small-molecular-bioprobes and proteomic analyses of signal transduction factors.

Research Project

Project/Area Number 15310156
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionRIKEN

Principal Investigator

OSADA Hiroyuki  RIKEN, Antibiotics Laboratory, Chief Scientist, Director of Antibiotics Laboratory, 長田抗生物質研究室, 主任研究員 (80160836)

Co-Investigator(Kenkyū-buntansha) WATANABE Nobumoto  RIKEN, Antibiotics Laboratory, Senior Research Scientist, 長田抗生物質研究室, 先任研究員 (90221689)
UEKI Masashi  RIKEN, Antibiotics Laboratory, Research Scientist, 長田抗生物質研究室, 研究員 (90312264)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2004: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2003: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsWeel / ubiquitin / cell cycle / Cdc2 / epoxytwinol / Wee1
Research Abstract

To elucidate biological functions, there are two ways of analysis. One is the information and structure based analysis such as genome science or structural biology. The other is the chemical biology based analysis, in which proteins essential for biological process are identified using their specific inhibitors. The purposes of the present research project are to generate chemicals ("bioprobes") useful for the latter type of study, which regulate the signal transduction pathway in cells, to identify biological target molecules of the bioprobes and to mine and analyze novel molecular targets. We have conducted the project for these purposes and have obtained many important results as follows.
1.Identification of molecular targets and characterization of bioprobes using proteomic analysis
(1)Development of photo-crosslinked small molecule microarrays to identify specific interacting molecules for target proteins in high throughput manner. Angew.Chem.Int.Ed.(2005)
(2)Elucidation of the bindi … More ng mechanism of phoslactomycin to its specific molecular target, protein phosphatase 2A.FEBS Lett.(2005)
(3)Identification of a mitochondria specific heat shock protein, Hsp60 as a target molecule for epolactaene and elucidation of the mechanism to inhibit HSP60's chaperone activity. Biochem.J.(2005)
2.Exploitation of novel bioprobes for the study of cellular signal transduction pathway
(1)Identification of the pathway and the gene cluster in an actinomycete for the biosynthesis of PP2A specific inhibitor, phoslactomycins, and development of a method to enhance the selective production of phoslactomycins in vivo. Tetrahedron (2003),J.Biol.Chem.(2003)
(2)Isolation and characterization of novel compounds, RK805 and epoxytwinol A as novel angiogenesis inhibitors. Tetrahedron (2004),Chem.Commun.(2005)
3.Molecular biology on novel molecular targets
(1)Elucidation of the mechanism of ubiquitin-proteasome dependent degradation of a cell cycle regulatory protein kinase, Weel. Proc.Natl.Acad.Sci.USA(2004) Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (126 results)

All 2005 2004 2003 Other

All Journal Article (107 results) Publications (19 results)

  • [Journal Article] Phoslactomycin targets cysteine-269 of the protein phosphatase 2A catalytic subunit in cells.2005

    • Author(s)
      T.Teruya et al.
    • Journal Title

      FEBS Lett. 579

      Pages: 2463-2468

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Total synthesis of Epoxyquinols A, B, and C and Epoxytwinol A and the reactivity of a 2H-pyran derivative as the diene component in the diels-alder reaction.2005

    • Author(s)
      M.Shoji et al.
    • Journal Title

      J.Org.Chem. 70

      Pages: 79-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity.2005

    • Author(s)
      Y.Nagumo et al.
    • Journal Title

      Biochm.J. 387

      Pages: 835-840

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epolactaene binds human Hsp60 Cys442 resulting in the inhibition of chaperone activity2005

    • Author(s)
      Y.Nagumo
    • Journal Title

      Biochem.J. 387

      Pages: 835-840

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Total synthesis of Epoxyquinols A, B, and C and Epoxytwinol A and the reactivity of a 2H-pyran derivative as the diene component in the diels-alder reaction2005

    • Author(s)
      M.Shoji
    • Journal Title

      J.Org.Chem. 70

      Pages: 79-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phoslactomycin targets cysteine-269 of the protein phosphatase 2A catalytic subunit in cells2005

    • Author(s)
      T.Teruya
    • Journal Title

      FEBS Lett. 579

      Pages: 2463-2468

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epolactaene binds human Hsp60 Cys^<442> resulting in the inhibition of chaperone activity.2005

    • Author(s)
      Nagumo, Y. et al.
    • Journal Title

      Biochem.J. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Total synthesis of epoxyquinols A, B, and C and epoxytwinol A and the reactivity of a 2H-pyran derivative as the diene component in the diels-alder reaction.2005

    • Author(s)
      Shoji, M. et al.
    • Journal Title

      J.Org.Chem. 70

      Pages: 79-91

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synthesis of a biotin-conjugate of phosmidosine O-ethyl ester as a G1 arrest antitumor drug.2004

    • Author(s)
      M.Sekine et al.
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 6343-6349

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A New VHR Dual-Specificity Protein Tyrosine Phosphatase Inhibitor from Dendrobium moniliforme.2004

    • Author(s)
      EY.Bae et al.
    • Journal Title

      Planta Med. 70

      Pages: 869-870

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-activity relationship of phosmidosine : importance of the 7,8-dihydro-8-oxoadenosine residue for antitumor activity.2004

    • Author(s)
      M.Sekine et al.
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 5193-5201

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exip, a splicing variant of p38a, participates in interleukin-1 receptor proximal complex and downregulates NF-kappaB pathway.2004

    • Author(s)
      Y.Yagasaki et al.
    • Journal Title

      FEBS Lett. 575

      Pages: 136-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Kaurene diterpene induces apoptosis in human leukemia cells partly through a caspase-8-dependent pathway.2004

    • Author(s)
      M.Kondoh et al.
    • Journal Title

      Pharmacol Exp Ther. 311

      Pages: 115-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amphidinolide h, a potent cytotoxic macrolide, covalently binds on actin subdomain 4 and stabilizes actin filament.2004

    • Author(s)
      T.Usui et al.
    • Journal Title

      Chem.Biol. 11

      Pages: 1269-1277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-based design of a selective heparanase inhibitor as an antimetastatic agent.2004

    • Author(s)
      K.Ishida et al.
    • Journal Title

      Mol.Cancer Ther. 3

      Pages: 1069-1077

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-activity relationships of epolactaene derivatives : structural requirements for inhibition of Hsp60 chaperone actitity.2004

    • Author(s)
      Y.Nagumo et al.
    • Journal Title

      Bioorg.Med.Chem. 14

      Pages: 4425-4429

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] RK-805, an endothelial-cell-growth inhibitor produced by Neosartorya sp., and a docking model with methionine aminopeptidase-2.2004

    • Author(s)
      Y.Asami et al.
    • Journal Title

      Tetrahedron 60

      Pages: 7085-7091

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heparanase as a molecular target of cancer chemotherapy.2004

    • Author(s)
      S.Simizu et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 553-558

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The phosphorylation status and anti-apoptotic activity of Bcl-2 are regulated by ERK and protein phosphatase 2A on the mitochondria.2004

    • Author(s)
      Y.Tamura et al.
    • Journal Title

      FEBS Lett. 569

      Pages: 249-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The anti-cancer natural product pironetin selectively targets Lys352 of alpha-tubulin.2004

    • Author(s)
      T.Usui et al.
    • Journal Title

      Chem.Biol. 11

      Pages: 799-806

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rational design and synthesis of novel heparan sulfate mimetic compounds as antiadhesive agents.2004

    • Author(s)
      K.Ishida et al.
    • Journal Title

      Bioorg.Med.Chem.Lett. 14

      Pages: 2505-2509

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ECH, an epoxycyclohexenone derivative that specifically inhibits Fas ligand-dependent apoptosis in CTL-mediated cytotoxicity.2004

    • Author(s)
      T.Mitsui et al.
    • Journal Title

      J.Immunol. 172

      Pages: 3428-3436

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] M-phase kinases induce phospho-depndent ubiquitination of somatic Weel by SCF^<β-TrCP>.2004

    • Author(s)
      N.Watanabe et al.
    • Journal Title

      Proc.Natl.Acad.Sci., USA. 101

      Pages: 4419-4424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel heparan sulfate mimetic compounds as antitumor agents.2004

    • Author(s)
      K.Ishida et al.
    • Journal Title

      Chem.& Biol. 11

      Pages: 367-377

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dephosphorylation of Bcl-2 by protein phasphatase 2A resulted in apoptosis resistance.2004

    • Author(s)
      S.Simizu et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 266-270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different reaction modes for the oxidative dimerization of epoxyquinols and epoxyquinones. importance of intermolecular hydrogen-bonding.2004

    • Author(s)
      M.Shoji et al.
    • Journal Title

      J.Org.Chem. 69

      Pages: 1548-1556

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exploitation of Heparanase Inhibitors from Microbial Metabolites Using an Efficient Visual Screening System.2004

    • Author(s)
      K.Ishida et al.
    • Journal Title

      J.Antibiot 57

      Pages: 136-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis of chemically stabilized phosmidosine analogues and the structure-activity relationship of phosmidosine.2004

    • Author(s)
      M.Sekine et al.
    • Journal Title

      J.Org.Chem. 69

      Pages: 314-326

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines.2004

    • Author(s)
      S.Simizu et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 2697-2703

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular interaction between cyclophilin D and adenine nucleotide translocase in cytochrome c release : does it determine whether cytochrome c release is dependent on permeability transition or not?2004

    • Author(s)
      K.Machida et al.
    • Journal Title

      Ann.N.Y.Acad.Sci. 1010

      Pages: 182-185

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines2004

    • Author(s)
      S.Simizu
    • Journal Title

      J.Biol.Chem. 279

      Pages: 2697-2703

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis of chemically stabilized phosmidosine analogues and the structure-activity relationship of phosmidosine2004

    • Author(s)
      M.Sekine
    • Journal Title

      J.Org.Chem. 69

      Pages: 314-326

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exploitation of Heparanase Inhibitors from Microbial Metabolites Using an Efficient Visual Screening System2004

    • Author(s)
      K.Ishida
    • Journal Title

      J.Antibiot. 57

      Pages: 136-142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Different reaction modes for the oxidative dimerization of epoxyquinols and epoxyquinones. importance of intermolecular hydrogen-bonding2004

    • Author(s)
      M.Shoji
    • Journal Title

      J.Org.Chem. 69

      Pages: 1548-1556

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dephosphorylation of Bcl-2 by protein phasphatase 2A resulted in apoptosis resistance2004

    • Author(s)
      S.Simizu
    • Journal Title

      Cancer Sci. 95

      Pages: 266-270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel heparan sulfate mimetic compounds as antitumor agents2004

    • Author(s)
      K.Ishida
    • Journal Title

      Chem. & Biol. 11

      Pages: 367-377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] M-phase kinases induce phospho-dependent ubiquitination of somatic Weel by SCF^<□-TrCP>, USA.2004

    • Author(s)
      N.Watanabe
    • Journal Title

      Proc.Natl.Acad.Sci. 101

      Pages: 4419-4424

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] ECH, an epoxycyclohexenone derivative that specifically inhibits Fas ligand-dependent apoptosis in CTL-mediated cytotoxicity2004

    • Author(s)
      T.Mitsui
    • Journal Title

      J.Immunol. 172

      Pages: 3428-3436

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Rational design and synthesis of novel heparan sulfate mimetic compounds as antiadhesive agents2004

    • Author(s)
      K.Ishida
    • Journal Title

      Bioorg.Med.Chem.Lett. 14

      Pages: 2505-2509

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The anti-cancer natural product pironetin selectively targets Lys352 of alpha-tubulin2004

    • Author(s)
      T.Usui
    • Journal Title

      Chem.Biol. 11

      Pages: 799-806

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The phosphorylation status and anti-apoptotic activity of Bcl-2 are regulated by ERK and protein phosphatase 2A on the mitochondria2004

    • Author(s)
      Y.Tamura
    • Journal Title

      FEBS Lett. 569

      Pages: 249-255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Heparanase as a molecular target of cancer chemotherapy2004

    • Author(s)
      S.Simizu
    • Journal Title

      Cancer Sci. 95

      Pages: 553-558

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] RK-805,an endothelial-cell-growth inhibitor produced by Neosartorya sp., and a docking model with methionine aminopeptidase-22004

    • Author(s)
      Y.Asami
    • Journal Title

      Tetrahedron. 60

      Pages: 7085-7091

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-activity relationships of epolactaene derivatives: structural requirements for inhibition of Hsp60 chaperone actitity2004

    • Author(s)
      Y.Nagumo
    • Journal Title

      Bioorg.Med.Chem. 14

      Pages: 4425-4429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-based design of a selective heparanase inhibitor as an antimetastatic agent2004

    • Author(s)
      K.Ishida
    • Journal Title

      Mol.Cancer Ther. 3

      Pages: 1069-1077

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Amphidinolide h, a potent cytotoxic macrolide, covalently binds on actin subdomain 4 and stabilizes actin filament2004

    • Author(s)
      T.Usui
    • Journal Title

      Chem.Biol. 11

      Pages: 1269-1277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Kaurene diterpene induces apoptosis in human leukemia cells partly through a caspase-8-dependent pathway2004

    • Author(s)
      M.Kondoh
    • Journal Title

      Pharmacol Exp Ther. 311

      Pages: 115-122

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Exip, a splicing variant of p38α, participates in interleukin-1 receptor proximal complex and downregulates NF-kappaB pathway2004

    • Author(s)
      Y.Yagasaki
    • Journal Title

      FEBS Lett. 575

      Pages: 136-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure-activity relationship of phosmidosine : importance of the 7,8-dihydro-8-oxoadenosine residue for antitumor activity2004

    • Author(s)
      M.Sekine
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 5193-5201

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A New VHR Dual-Specificity Protein Tyrosine Phosphatase Inhibitor from Dendrobium moniliforme2004

    • Author(s)
      EY.Bae
    • Journal Title

      Planta Med. 70

      Pages: 869-870

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis of a biotin-conjugate of phosmidosine O-ethyl ester as a G1 arrest antitumor drug2004

    • Author(s)
      M.Sekine
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 6343-6349

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis of a biotin-conjugate of phosmidosine O-ethyl ester as a G1 arrest antitumor drug.2004

    • Author(s)
      Sekine, M. et al.
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 6343-6349

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure-activity relationship of phosmidosine : importance of the 7,8-dihydro-8-oxoadenosine residue for antitumor activity.2004

    • Author(s)
      Sekine, M. et al.
    • Journal Title

      Bioorg.Med.Chem. 12

      Pages: 5193-5201

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A new VHR dual-specificity protein tyrosine phosphatase inhibitor from dendrobium moniliforme.2004

    • Author(s)
      Bae, EY. et al.
    • Journal Title

      Planta Med. 70

      Pages: 869-870

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Exip, a splicing variant of p38α, participates in interleukin-1 receptor proximal complex and downregulates NF-kappaB pathway.2004

    • Author(s)
      Yagasaki, Y. et al.
    • Journal Title

      FEBS Lett. 575

      Pages: 136-140

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Kaurene diterpene induces apoptosis in human leukemia cells partly through a caspase-8-dependent pathway.2004

    • Author(s)
      Kondoh, M. et al.
    • Journal Title

      Pharmacol Exp.Ther. 311

      Pages: 115-122

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Amphidinolide h, a potent cytotoxic macrolide, covalently binds on actin subdomain 4 and stabilizes actin filament.2004

    • Author(s)
      Usui, T. et al.
    • Journal Title

      Chem.Biol. 11

      Pages: 1269-1277

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure-based design of a selective heparanase inhibitor as an antimetastatic agent.2004

    • Author(s)
      Ishida, K. et al.
    • Journal Title

      Mol.Cancer Ther. 3

      Pages: 1069-1077

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure-activity relationships of epolactaene derivatives : structural requirements for inhibition of Hsp60 chaperone activity.2004

    • Author(s)
      Nagumo, Y. et al.
    • Journal Title

      Bioorg.Med.Chem. 14

      Pages: 4425-4429

    • Related Report
      2004 Annual Research Report
  • [Journal Article] RK-805, an endothelial-cell-growth inhibitor produced by Neosartorya sp., and a docking model with methionine aminopeptidase-2.2004

    • Author(s)
      Asami, Y. et al.
    • Journal Title

      Tetrahedron. 60

      Pages: 7085-7091

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Heparanase as a molecular target of cancer chemotherapy.2004

    • Author(s)
      Simizu, S. et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 553-558

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The phosphorylation status and anti-apoptotic activity of Bcl-2 are regulated by ERK and protein phosphatase 2A on the mitochondria.2004

    • Author(s)
      Tamura, Y. et al.
    • Journal Title

      FEBS Lett. 569

      Pages: 249-255

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The anti-cancer natural product pironetin selectively targets Lys352 of alpha-tubulin.2004

    • Author(s)
      Usui, T. et al.
    • Journal Title

      Chem.Biol. 11

      Pages: 799-806

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rational design and synthesis of novel heparan sulfate mimetic compounds as antiadhesive agents.2004

    • Author(s)
      Ishida, K. et al.
    • Journal Title

      Bioorg.Med.Chem.Lett. 14

      Pages: 2505-2509

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ECH, an epoxycyclohexenone derivative that specifically inhibits Fas ligand-dependent apoptosis in CTL-mediated cytotoxicity.2004

    • Author(s)
      Mitusi, T. et al.
    • Journal Title

      J.Immunol. 172

      Pages: 3428-3436

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel heparan sulfate mimetic compounds as antitumor agents.2004

    • Author(s)
      Ishida, K. et al.
    • Journal Title

      Chem.& Biol. 11

      Pages: 367-377

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dephosphorylation of Bcl-2 by protein phasphatase 2A resulted in apoptosis resistance.2004

    • Author(s)
      Simizu, S. et al.
    • Journal Title

      Cancer Sci. 95

      Pages: 266-270

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Different reaction modes for the oxidative dimerization of epoxyquinols and epoxyquinones. importance of intermolecular hydrogen-bonding.2004

    • Author(s)
      Shoji, M. et al.
    • Journal Title

      J.Org.Chem. 69

      Pages: 1548-1556

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Exploitation of heparanase inhibitors from microbial metabolites using an efficient visual screening system.2004

    • Author(s)
      Ishida, K. et al.
    • Journal Title

      J.Antibiot. 57

      Pages: 136-142

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synthesis of chemically stabilized phosmidosine analogues and the structure-activity relationship of phosmidosine.2004

    • Author(s)
      Sekine, M. et al.
    • Journal Title

      J.Org.Chem. 69

      Pages: 314-326

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Reversal of breast cancer resistance protein-mediated drug resistance by tryprostatin A.2003

    • Author(s)
      H.Woehlecke et al.
    • Journal Title

      Int.J.Cancer 107

      Pages: 721-728

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The lafora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a Nifu-like domain.2003

    • Author(s)
      S.Ganesh et al.
    • Journal Title

      Hum.Mol.Genet 12

      Pages: 2359-2368

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small molecule interactions.2003

    • Author(s)
      N.Kanoh et al.
    • Journal Title

      Angew.Chem.Int.Ed. 42

      Pages: 5584-5587

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel non-peptide inhibitors targeting death receptor-mediated apoptosis.2003

    • Author(s)
      H.Kakeya et al.
    • Journal Title

      Bioorg.Med.Chem.Lett. 13

      Pages: 3743-3746

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis and cell cycle inhibition of the peptide enamide natural products terpeptin and the aspergillamides.2003

    • Author(s)
      S.Su et al.
    • Journal Title

      Tetrahedron. 59

      Pages: 8931-8946

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Renal abnormalities in mice Caused by insufficiency of p38α.2003

    • Author(s)
      M.Maruyama et al.
    • Journal Title

      J.Rec.Sig.Trans. 23

      Pages: 173-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reaction modes of oxidative dimerization of epoxycyclohexenols.2003

    • Author(s)
      M.Shoji et al.
    • Journal Title

      Tetrahedron Lett. 44

      Pages: 7205-7207

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster.2003

    • Author(s)
      N.Palaniappan et al.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 35552-35557

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncate.2003

    • Author(s)
      F.Nagashima et al.
    • Journal Title

      Planta Medica 69

      Pages: 377-379

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biosynthesis of phoslactomycins : cyclohexanecarboxylic acid as the starter unit.2003

    • Author(s)
      Y.Sekiyama et al.
    • Journal Title

      Tetrahedron 59

      Pages: 7465-7471

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Asymmetric total synthesis of pseurotin A.2003

    • Author(s)
      Y.Hayashi et al.
    • Journal Title

      Organic Lett. 5

      Pages: 2287-2290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of heparanase in human tumor cell lines and human head and neck tumors.2003

    • Author(s)
      S.Sinizu et al.
    • Journal Title

      Cancer Lett. 193

      Pages: 83-89

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transmembrane domain of Bcl-2 is required for inhibition of ceramide synthesis, but not cytochrome c release in the pathway of inostamysin-induced apoptosis.2003

    • Author(s)
      M.Kawatani et al.
    • Journal Title

      Exp.Cell.Res. 286

      Pages: 57-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and application of bioprobes for mammalian cell cycle analyses.2003

    • Author(s)
      H.Osada
    • Journal Title

      Curr.Med.Chem. 10

      Pages: 727-732

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epoxycyclohexenone inhibits fas-mediated apoptosis by blocking activation of pro-caspase-8 in the death-inducing signaling complex.2003

    • Author(s)
      Y.Miyake et al.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 11213-11220

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel action of terpendole E on the motor activity of mitotic kinesin Eg5.2003

    • Author(s)
      J.Nakazawa et al.
    • Journal Title

      Chem.& Biol. 10

      Pages: 131-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nordihydroguaiaretic acid, of a new family of microtubule-stabilizing agents, shows effects differentiated from paclitaxel.2003

    • Author(s)
      M.Nakamura et al.
    • Journal Title

      Biosci.Biotechnol.Biochem. 67

      Pages: 151-157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Disruption of a single copy of the p38α MAP kinase gene leads to cardioprotection against ischemia-reperfusion.2003

    • Author(s)
      K.Otsu et al.
    • Journal Title

      Biochem.Biophys.Res Commun. 302

      Pages: 56-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Disruption of a single copy of the p38α MAP kinase gene leads to cardioprotection against ischemia-reperfusion2003

    • Author(s)
      K.Otsu
    • Journal Title

      Biochem.Biophys.Res Commun. 302

      Pages: 56-60

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nordihydroguaiaretic acid, of a new family of microtubule-stabilizing agents, shows effects differentiated from paclitaxel2003

    • Author(s)
      M.Nakamura
    • Journal Title

      Biosci.Biotechnol.Biochem. 67

      Pages: 151-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel action of terpendole E on the motor activity of mitotic kinesin Eg52003

    • Author(s)
      J.Nakazawa
    • Journal Title

      Chem. & Biol. 10

      Pages: 131-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epoxycyclohexenone inhibits fas-mediated apoptosis by blocking activation of pro-caspase-8 in the death-inducing signaling complex2003

    • Author(s)
      Y.Miyake
    • Journal Title

      J.Biol.Chem. 278

      Pages: 11213-11220

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development and application of bioprobes for mammalian cell cycle analyses2003

    • Author(s)
      H.Osada
    • Journal Title

      Curr.Med.Chem. 10

      Pages: 727-732

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Transmembrane domain of Bcl-2 is required for inhibition of ceramide synthesis, but not cytochrome c release in the pathway of inostamysin-induced apoptosis2003

    • Author(s)
      M.Kawatani
    • Journal Title

      Exp.Cell.Res. 286

      Pages: 57-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of heparanase in human tumor cell lines and human head and neck tumors2003

    • Author(s)
      S.Simizu
    • Journal Title

      Cancer Lett. 193

      Pages: 83-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Asymmetric total synthesis of pseurotin A2003

    • Author(s)
      Y.Hayashi
    • Journal Title

      Organic Lett. 5

      Pages: 2287-2290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Biosynthesis of phoslactomycins : cyclohexanecarboxylic acid as the starter unit2003

    • Author(s)
      Y.Sekiyama
    • Journal Title

      Tetrahedron 59

      Pages: 7465-7471

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Apoptosis -inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata2003

    • Author(s)
      F.Nagashima
    • Journal Title

      Planta Medica 69

      Pages: 377-379

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhancement and selective production of phoslactomycin B, a protein phosphatase IIa inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster2003

    • Author(s)
      N.Palaniappan
    • Journal Title

      J.Biol.Chem. 278

      Pages: 35552-35557

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reaction modes of oxidative dimerization of epoxycyclohexenols2003

    • Author(s)
      M.Shoji
    • Journal Title

      Tetrahedron Lett. 44

      Pages: 7205-7207

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Renal abnormalities in mice Caused by insufficiency of p38□2003

    • Author(s)
      M.Maruyama
    • Journal Title

      J.Rec.Sig.Trans. 23

      Pages: 173-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis and cell cycle inhibition of the peptide enamide natural products terpeptin and the aspergillamides2003

    • Author(s)
      S.Su
    • Journal Title

      Tetrahedron. 59

      Pages: 8931-8946

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel non-peptide inhibitors targeting death receptor-mediated apoptosis2003

    • Author(s)
      H.Kakeya
    • Journal Title

      Bioorg.Med.Chem.Lett. 13

      Pages: 3743-3746

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small molecule interactions2003

    • Author(s)
      N.Kanoh
    • Journal Title

      Angew.Chem.Int.Ed. 42

      Pages: 5584-5587

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The la fora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a Nifu-like domain2003

    • Author(s)
      S.Ganesh
    • Journal Title

      Hum.Mol.Genet. 12

      Pages: 2359-2368

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reversal of breast cancer resistance protein-mediated drug resistance by tryprostatin A2003

    • Author(s)
      H.Woehlecke
    • Journal Title

      Int.J.Cancer 107

      Pages: 721-728

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular interaction between cyclophilin D and adenine nucleotide translocase in cytochrome c release : does it determine whether cytochrome c release is dependent on permeability transition or not?2003

    • Author(s)
      K.Machida
    • Journal Title

      Ann.N.Y.Acad.Sci. 1010

      Pages: 182-185

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Watanabe, N. et al.: "M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbTrCP."Proc.Natl.Acad.Sci., USA.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Simizu, S. et al.: "Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines."J.Biol.Chem.. 279. 2697-2703 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Woehlecke, H. et al.: "Reversal of breast cancer resistance protein-mediated drug resistance by tryprostatin A."Int.J.Cancer.. 107. 721-728 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ganesh, S. et al.: "The lafora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a Nifu-like domain."Hum.Mol.Genet.. 12. 2359-2368 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kanoh, N. et al.: "Immobilization of natural products on glass slides by using a photoaffinity reaction and the detection of protein-small molecule interactions."Angew.Chem.Int.Ed.. 42. 5584-5587 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kakeya, H. et al.: "Novel non-peptide inhibitors targeting death receptor-mediated apoptosis."Bioorg.Med.Chem.Lett.. 13. 3743-3746 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Su, S.et al.: "Synthesis and cell cycle inhibition of the peptide enamide natural products terpeptin and the aspergillamides."Tetrahedron.. 59. 8931-8946 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maruyama, M. et al.: "Renal abnormalities in mice Caused by insufficiency of p38 α."J.Rec.Sig.Trans.. 23. 173-183 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shoji, M. et al.: "Reaction modes of oxidative dimerization of epoxycyclohexenols."Tetrahedron Lett.. 44. 7205-7207 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Palaniappan, N. et al.: "Enhancement and selective production of phoslactomycin B, a protein phosphatase iia inhibitor, through identification and engineering of the corresponding biosynthetic gene cluster."J.Biol.Chem.. 278. 35552-35557 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagashima, F. et al.: "Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort jungermannia truncata."Planta Medica. 69. 377-379 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sekiyama, Y. et al.: "Biosynthesis of phoslactomycins : cyclohexanecarboxylic acid as the starter unit."Tetrahedron. 59. 7465-7471 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayashi, Y. et al.: "Asymmetric total synthesis of pseurotin A."Organic Lett.. 5. 2287-2290 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Simizu, S. et al.: "Expression of heparanase in human tumor cell lines and human head and neck tumors."Cancer Lett.. 193. 83-89 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kawatani, M. et al.: "Transmembrane domain of Bcl-2 is required for inhibition of ceramide synthesis, but not cytochrome c release in the pathway of inostamysin-induced apoptosis."Exp. Cell Res.. 286. 57-66 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Osada, H.: "Development and application of bioprobes for mammalian cell cycle analyses."Cur.Med.Chem.. 10. 727-732 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyake, Y. et al.: "Epoxycyclohexenone inhibits fas-mediated apoptosis by blocking activation of pro-caspase-8 in the death-inducing signaling complex."J.Biol.Chem.. 278. 11213-11220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakazawa, J. et al.: "A novel action of terpendole E on the motor activity of mitotic kinesin Eg5."Chem. & Biol.. 10. 131-137 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura, M. et al.: "Nordihydroguaiaretic acid, of a new family of microtubule-stabilizing agents, shows effects differentiated from paclitaxel."Biosci.Biotechnol.Biochem.. 67. 151-157 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi