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Self oscillatory growth of ice crystal of ice in the antifreeze protein solution and its dynamics

Research Project

Project/Area Number 15340136
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics/Chemical physics
Research InstitutionHokkaido University

Principal Investigator

FURUKAWA Yoshinori  Hokkaido Univ., Institute of Low Temperature Science, Professor, 低温科学研究所, 教授 (20113623)

Co-Investigator(Kenkyū-buntansha) KATAGIRI Chihiro  Hokkaido Univ., Institute of Low Temperature Science, Assistant Professor, 低温科学研究所, 助教授 (90002245)
YOKOYAMA Etsuro  Gakushuin University, Computer Center, Professor, 計算機センター, 教授 (40212302)
MATSUURA Yoshiki  Osaka University, Protein Research Institute, Professor, 蛋白質研究所, 教授 (90029968)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2003: ¥8,100,000 (Direct Cost: ¥8,100,000)
KeywordsIce crystal / Antifreeze glycoprotein / Crystal growth kinetics / Self-oscillatory / Interface adsorption / Protein conformation / Kinetic loop / Fluorescence / 結晶成長 / 不凍タンパク質 / カイネティクス / 蛍光観察 / 拡散場 / 界面成長ステップ / 結晶成長機構 / 結晶成長制御
Research Abstract

Several kinds of ice crystal growth experiments were conducted in a solution of antifreeze glycoprotein molecules which were labeled by fluorescent material. One directional growth of ice clearly showed the oscillation of growth rates and periodic incorporation of AFGP molecules depending on the growth rate. This is the first direct observation of striation formation inside the crystal. Free growth experiments of ice single crystals also showed the oscillations of growth rates. The growth mode was ON-OFF type oscillation for the prismatic plane, whereas the continuous oscillation was observed for the basal plane.
Secondary structures (conformations) of AFGP molecules under the adsorbed state at ice/water interface were examined using Attenuated Total Reflection type FTIR technique. The FTIR signal indicated that the AFGP molecules with a-helix conformation should be dominant at the ice/water interface. Since the dominant conformations of molecules in the solution showed the random state, this result means that the conformation change occur in order to strongly adsorb to the interface.
A model to explain the oscillatory growth of ice crystal was also developed on the basis of the concept of kinetic loop, which was derived from the hysteresis relationships between the growth rate and the driving force. This model also suggest that the AFGP molecule adsorption on the interface should occur in two states, namely week adsorption state and tight adsorption state, which is consistent with the experimental result obtained by ATR-FTIR measurement.
As a result, we clarified the dynamics of self-oscillatory growth of ice crystal in the AFGP solution. Understanding the crystal growth dynamics strongly affected by the adsorption of AFGP molecules will play an important role to clarify the antifreeze function of these proteins.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (48 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (45 results) Publications (3 results)

  • [Journal Article] Diffusion,incorporation,and segregation of antifreeze proteins at the ice/solution interface.2007

    • Author(s)
      S.Zepeda, H.Nakaya, Y.Uda, E.Yokoyama, Y.Furukawa
    • Journal Title

      Proceedings on International Conference on Physics and Chemistry of Ice, 印刷中

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Growth kinetics on interface between(2021)plane of ice and water investifated by a molecular dynamics simulation.2007

    • Author(s)
      H.Nada, Y.Furukawa
    • Journal Title

      Proceedings on International Conference on Physics and Chemistry of Ice, 印刷中

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diffusion, incorporation, and segregation of antifreeze proteins at the ice/solution interface.2007

    • Author(s)
      S.Zepeda, H.Nakaya, Y.Uda, E.Yokoyama, Y.Furukawa
    • Journal Title

      Proceedings on International Conference on Physics and Chemistry of Ice

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Growth kinetics on interface between (2021) plane of ice and water investigated by a molecular dynamics simulation.2007

    • Author(s)
      H.Nada, Y.Furukawa
    • Journal Title

      Proceedings on International Conference on Physics and Chemistry of Ice

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 合成不凍糖蛋白質を用いた膵島凍結保存法の開発2006

    • Author(s)
      松本秀一朗, 松下通明, 森田恒彦, 蒲池浩文, 古川義純, 西村伸一郎, 藤堂省
    • Journal Title

      医学の歩み 216

      Pages: 481-482

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of synthetic antifreeze glycoprotein analogue on Islet cell survival and function during cryopreservation.2006

    • Author(s)
      S.Matsumoto, M.Matsusita, T.Morita, H.Kamachi, S.Tsukiyama, Y.Furukawa, S.Koshida, Y.Tachibana, S.Nishimura, S.Todo
    • Journal Title

      Cryobiology 52

      Pages: 90-98

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Freezing storage method of Islet cell by synthetic antifreeze protein2006

    • Author(s)
      S.Matsumoto, M.Matsushita, T.Morita, Y.Furukawa
    • Journal Title

      Igaku-no-Ayumi 216

      Pages: 481-482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of synthetic antifreeze glycoprotein analogue on Islet cell survival and function during cryopreservation.2006

    • Author(s)
      S.Matsumoto, Y.Furukawa, et al.
    • Journal Title

      Cryobiology 52

      Pages: 90-98

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Growth patterns and interfacial kinetic supercooling at ice/water interfaces at which anti-freeze glycoprotein molecules are adsorbed.2005

    • Author(s)
      Y.Furukawa, N.Inohara, E.Yokoyama
    • Journal Title

      J.Crystal Growth 275

      Pages: 167-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 氷点下でも凍らない魚-不凍タンパク質が氷の結晶成長を支配する-2005

    • Author(s)
      古川 義純
    • Journal Title

      バイオニクス 1-3

      Pages: 54-66

    • NAID

      10016160756

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 氷の結晶成長をシュミレートするための新しい水分子モデル2005

    • Author(s)
      灘浩樹, J.P.van der Eerden, 古川義純
    • Journal Title

      低温科学. 64

      Pages: 77-88

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 微小重力での結晶成長"その場"観察,2005

    • Author(s)
      塚本勝男, 西村良浩, 横山悦郎
    • Journal Title

      可視化情報 25

      Pages: 156-163

    • NAID

      10016586000

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antisotropy in growth linetics at interfaces between proton-disordered hexagonal ice and water : molecular dynamics study using the six-site model of H2O.2005

    • Author(s)
      H.Nada, Y.Furukawa
    • Journal Title

      J.Crystal Growth 283

      Pages: 242-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effective distribution coefficients of an ideal solid solution crystal : Monte Carlo simulation,2005

    • Author(s)
      K.Matsumoto, T.Irisawa, M.Kitamura, E.Yokoyama
    • Journal Title

      J.Crystal Growth 276

      Pages: 635-642

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of synthetic antifreeze glycoprotein analogue on Islet cell survival and function during cryopreservation.2005

    • Author(s)
      S.Matsumoto, M.Matsusita, T.Morita, H.Kamachi, S.Tsukiyama, Y.Furukawa, S.Koshida, Y.Tachibana, S.Nishimura, S.Todo
    • Journal Title

      Cryobiology,52(2006)90-98. 52

      Pages: 90-98

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Growth patterns and interfacial kinetic supercooling at ice/water interfaces at which anti-freeze glycoprotein molecules are adsorbed.2005

    • Author(s)
      Y.Furukawa, N.Inohara, E.Yokoyama
    • Journal Title

      J. Crystal Growth 275

      Pages: 167-174

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Unfrozen fishes under subzero temperatures2005

    • Author(s)
      Y.Furukawa
    • Journal Title

      Bionics Vol.1, No.3

      Pages: 64-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] New model of water molecule for ice crystal growth simulation2005

    • Author(s)
      H.Nada, J.P.van der Eerden, Y.Furukawa
    • Journal Title

      Low Temp. Science 64

      Pages: 77-88

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In situ observation of crystal growth under microgravity condition2005

    • Author(s)
      K.Tsukamoto, Y.Nishimura, E.Yokoyama
    • Journal Title

      J. Visualization Soc. of Jpn. 25

      Pages: 156-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anisotropy in growth kinetics at interfaces between proton-disordered hexagonal ice and water : molecular dynamics study using the six-site model of H2O.2005

    • Author(s)
      H.Nada, Y.Furukawa
    • Journal Title

      J. Crystal Growth 283

      Pages: 242-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effective distribution coefficients of an ideal solid solution crystal : Monte Carlo simulation2005

    • Author(s)
      K.Matsumoto, T.Irisawa, M.Kitamura, E.Yokoyama, Yoshinao Kumagai, Akinori Koukitu
    • Journal Title

      J. Crystal Growth 276

      Pages: 635-642

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anisotropy in growth kinetics at interfaces between proton-disordered hexagonal ice and water : a molecular dynamics study using the six-site model of H2O.2005

    • Author(s)
      H.Nada, Y.Furukawa
    • Journal Title

      J.Crystal Growth 283

      Pages: 242-256

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Morphology instability on an ice crystal growing in supercooled water.2005

    • Author(s)
      Y.Furukawa, E.Yokoyama, W.Shimada
    • Journal Title

      Current Topic in Crystal Growth, (ed. Y.Hayakawa and Y.Furukawa,)(Research Trends, India)

      Pages: 165-186

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Growth patterns and interfacial kinetic supercooling at ice/water interfaces at which anti-freeze glycoprotein molecules are adsorbed2005

    • Author(s)
      Y.Furukawa, N.Inohara, E.Yokoyama
    • Journal Title

      J.Crystal Growth In press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Visualization of a thermal diffusion field around a single ice crystal growing in supercooled water under a short-term microgravity condition.2004

    • Author(s)
      Y.Furukawa, E.Yokoyama, Y.Nishimura, J.Ohtsubo, N.Inohara, S.Nakatsubo
    • Journal Title

      J.Jpn Soc.Microgravity Appl. 21

      Pages: 196-201

    • NAID

      10013610123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A clear observation of cristal growth if ice from water in a molecular dynamics simulation with a six-site potential model of H2O.2004

    • Author(s)
      H.Nada, J.P.van der Eerden, Y.Furukawa
    • Journal Title

      J.Crystal Growth 266

      Pages: 297-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Estimation of interlamellar water molecules in sphingomyelin bilayer systems as studied by DSC and X-ray diffraction,2004

    • Author(s)
      Kodama M., Abe M., Kawasaki Y., Hayashi K., Ohira S., Nozaki H., Katagiri C., Inoue K., Takahashi H.
    • Journal Title

      Thermochimica Acta 416

      Pages: 105-111

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two-dimensional diffraction study of ice crystallization in polymer gels.2004

    • Author(s)
      N.Murase, S.Abe, H.Takahashi H., Katagiri C., Kikegawa T.
    • Journal Title

      CyroLetters 25

      Pages: 227-234

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Polyunsaturated fatty acid,eicosapentaenoic acid,mediates larbal-pupal and pupal-adult development in the malarial vector mosquito,Anopheles Stephensi.2004

    • Author(s)
      A.Moribayashi, H.Sugie, C.Katagiri, K.Uchida, M.Kobayashi, N.Agui
    • Journal Title

      Med.Entomol.Zool. 55

      Pages: 59-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Epicuticular wax of large and small white butterflies,Pieris brassicae and P.rapae crucivora : qualitative and quantitative comparison between diapause and non-diapause pupae,2004

    • Author(s)
      J.Kaneko, C.Katagiri
    • Journal Title

      Naturwissenschaften 91

      Pages: 320-323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The basis for colorless hemolymph and cocoons in the Y-generessive Bombyx mori mutants : a defect in the cellular uptake of carotenoids,2004

    • Author(s)
      K.Tsuchida, C.Katagiri, Y.Tanaka, H.Tabunoki, R.Sato, H.Maekawa, N.Tanaka, Y.Banno, H.Fujii, M.Wells, A.E.Jouni
    • Journal Title

      J.Insect Physiol. 50

      Pages: 975-983

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 氷結晶成長に対する不純物の効果2004

    • Author(s)
      古川 義純
    • Journal Title

      低温生物工学会誌 50

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 氷の円盤結晶の形態不安定化2004

    • Author(s)
      古川 義純, 横山 悦郎
    • Journal Title

      日本マイクログラビティ応用学会誌 21

      Pages: 217-223

    • NAID

      10013610170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Visualization of a thermal diffusion field around a single ice crystal growing in supercooled water under a short-term microgravity condition.2004

    • Author(s)
      Y.Furukawa, E.Yokoyama, Y.Nishimura, J.Ohtsubo, N.Inohara, S.Nakatsubo
    • Journal Title

      J. Jpn Soc. Microgravity Appl. 21

      Pages: 196-201

    • NAID

      10013610123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A clear observation of crystal growth if ice from water in a molecular dynamics simulation with a six-site potential model of H2O.2004

    • Author(s)
      H.Nada, J.P.van der Eerden, Y.Furukawa
    • Journal Title

      J. Crystal Growth 266

      Pages: 297-302

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Estimation of interlamellar water molecules in sphingomyelin bilayer systems as studied by DSC and X-ray diffraction2004

    • Author(s)
      Kodama M., Abe M., Kawasaki Y, Hayashi K., Ohira S., Nozaki H., Katagiri C., Inoue K., Takahashi H.
    • Journal Title

      Thermochimica Acta 416

      Pages: 105-111

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two-dimensional diffraction study of ice crystallization in polymer gels.2004

    • Author(s)
      N.Murase, S.Abe, H.Takahashi H., Katagiri C., Kikegawa T.
    • Journal Title

      CryoLetters 25

      Pages: 227-234

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Polyunsaturated fatty acid, eicosapentaenoic acid, mediates larval-pupal and pupal-adult development in the malarial vector mosquito, Anopheles Stephensi.2004

    • Author(s)
      A.Moribayashi, H.Sugie, C.Katagiri, K.Uchida, M.Kobayashi, N.Agui.
    • Journal Title

      Med. Entomol. Zool. 55

      Pages: 59-66

    • NAID

      110003819406

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Epicuticular wax of large and small white butterflies, Pieris brassicae and P. rapae crucivora : qualitative and quantitative comparison between diapause and non-diapause pupae2004

    • Author(s)
      J.Kaneko, C.Katagiri
    • Journal Title

      Naturwissenschaften 91

      Pages: 320-323

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The basis for colorless hemolymph and cocoons in the Y-gene recessive Bombyx mori mutants : a defect in the cellular uptake of carotenoids2004

    • Author(s)
      K.Tsuchida, C.Katagiri, Y.Tanaka, H.Tabunoki, R.Sato, H.Maekawa, N.Takada, Y.Banno, H.Fujii, M.Wells, A.E.Jouni
    • Journal Title

      J. Insect Physiol. 50

      Pages: 975-983

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Impurity effect for ice crystal growth in supercooled water2004

    • Author(s)
      Y.Furukawa
    • Journal Title

      J. Biolo. Eng. 50

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphological instability on an ice disk crystal2004

    • Author(s)
      Y.Furukawa, E.Yokoyama
    • Journal Title

      J. Jpn Soc. Microgravity Appl. 21

      Pages: 217-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Visualization of a thermal diffusion field around a single ice crystal growing in supercooled water under a short-term microgravity condition.2004

    • Author(s)
      Y.Furukawa, E.Yokoyama, et al.
    • Journal Title

      J.Jpn Soc.Microgravity Appl. 21

      Pages: 196-201

    • NAID

      10013610123

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 氷の結晶成長ダイナミクスに対する凍結抑制タンパク質の効果2003

    • Author(s)
      古川義純, 西村良浩, 横山悦郎, 猪原直美
    • Journal Title

      日本結晶成長学会誌 30

      Pages: 21-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of antifreeze protein for ice crystal growth dynamics2003

    • Author(s)
      Y.Furukawa, Y.Nishimura, E.Yokoyama, N.Inohara
    • Journal Title

      J. Jpn. Soc. Cryst. Growth 30

      Pages: 21-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] K.Nagashima, Y.Furukawa: "Effects of gravity on pattern formation in directional growth of ice crystals"Canadian J.of Physics. 81, 1/2. 99-105 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] N.Takenaka, S.Furuya, K.Sato, H.Bandow, Y.Maeda, Y.Furukawa: "Rapid reaction of sulfide with hydrogen peroxide and formation of different final products by"International Journal of Chemical Kinetics. 35. 198-205 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] H.Nada, J.P.Van der Eerden, Y.Furukawa: "A clear observation of crystal growth if ice from water in a molecular dynamics simulation with a six-site potential model of H_2O"J.Crystal Growth. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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