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Study on a New Open Tubular Capillary Liquid Chromatography(OTCLC) System Based on Micro-Phase Separation of Mixed Solvents

Research Project

Project/Area Number 15350046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionSaga University

Principal Investigator

TABATA Masaaki  Saga University, Faculty of Science and Technology, Professor, 理工学部, 教授 (40039285)

Co-Investigator(Kenkyū-buntansha) NISHIMOTO June  Saga University, Instrumental Analytical Center, Associate Professor, 総合分析実験センター, 助教授 (80253582)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2004: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2003: ¥7,200,000 (Direct Cost: ¥7,200,000)
Keywordsopen tubular capillary liquid chromatography / microheterogeneity / mixed solvents / salting-out / acetonitrile / ナフトール化合物の分離 / ナノパーティクル / 水-アセトニトリル / 相分離 / オープンチュウブクロマトグラフィー / オープンチューブ液体クロマトグラフィー
Research Abstract

The proposed open tubular capillary liquid chromatography(OTCLC) system utilizes the recent discoveries of "microheterogeneity" in some organic-water mixed solvents and salting-out phase separation phenomena observed in these mixed solvents. For example, acetonitrile is miscible with water at any ratio ; heterogeneities, however, have been observed at a molecular level in its water mixtures. When an acetonitrile-water mixture of suitable salt concentration is pumped into such a capillary micro-phase separation is likely happening in the vicinity of the capillary wall, in a water-enriched aqueous phase attached to the capillary inner wall. In other words, a liquid membrane that has considerably different properties from the mobile phase is dynamically formed on the inner wall of the capillary.
When a mixture of analytes is injected into the capillary, participation of the analytes occurs between the mobile phase and the liquid membrane, providing the separation power of the proposed chromatograpy system.
Chromatographs clearly demonstrated the ability of separating neutral compounds from charged compounds by the OTCLC system. Moreover, it was also possible to separate compounds of similar molecular structure but different charges. The elution orders can readily be explained by the retention mechanism. p-Nitroaniline is effectively extracted to the upper (acetonitrile-enriched) phase while 2,7-NDS remains in the lower (water-enriched) phase, confirming the elution order observed. The high concentration of acetonitrile content in the mobile phase slightly increased the elution time of 1-naphthol but significantly increased the elution time of 2,6-naphtalene disulfonic acid. More interestingly, at the absence of NaCl, no separation in both cases (AN/aq 9/1 and AN/aq 8/2) were observed, demonstrating the necessity of additional electrolyte for the micro-phase separation in the capillary tubing.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (44 results)

All 2005 2004 2003 Other

All Journal Article (38 results) Publications (6 results)

  • [Journal Article] Change in Conformation of J-Aggregate 5,10,15,20-tetrakis(4-sulfonatophenyl) porphyrin(H_2TPPS) by Addition of Nonionic Surfactant(Triton X-100)2005

    • Author(s)
      Nattakarn Wangfuengkanagul
    • Journal Title

      Chem.Lett.

      Pages: 38-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Formation of Gold Nanoparticles by Good's Buffers2005

    • Author(s)
      Habib
    • Journal Title

      Bull.Chem.Soc.Jpn. 78

      Pages: 262-269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA Cleavage by Good's Buffers in the Presence of Au(III)2005

    • Author(s)
      M.Tabata
    • Journal Title

      Bull.Chem.Soc.Jpn. (in press)

    • NAID

      10016580145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Change in Conformation of J-aggregate 5,10,15,20-tetrakis (4-sulfonatophenyl)porphyrin (H_2TPPS) by Addition of Nonionic Surfactant (Triton X-100)2005

    • Author(s)
      Nattakarn Wangfuengkanagul, Masaaki Tabata
    • Journal Title

      Chem.Lett.

      Pages: 38-39

    • NAID

      10014217809

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Formation of gold Nanoparticles by Good's Buffers2005

    • Author(s)
      Habib, M.Tabata, Y.G.Wu
    • Journal Title

      Bull.Chem.Soc.Jpn. 78

      Pages: 262-269

    • NAID

      10014366447

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA Cleavage by Good's Buffers in the Presence of Au(III)2005

    • Author(s)
      M.Tabata, A.Habib
    • Journal Title

      Bull.Chem.Soc.Jpn. (in press)

    • NAID

      10016580145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Kinetics of the Complexation of Nickel(II) with 1,10- Phenan- throline and its Derivatives in Acetonitrile-EWater Mixed Solvents2005

    • Author(s)
      Y.G.Wu
    • Journal Title

      Inorganic Chimica Acta 358/6

      Pages: 1823-1829

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Change in Conformation of J-aggregate 5,10,15,20-tetrakis (4-sulfonatophenyl) porphyrin (H_2TPPS) by Addition of Nonionic Surfactant(Triton X-100)2005

    • Author(s)
      Nattakarn Wangfuengkanagul
    • Journal Title

      Chemistry Letters 34

      Pages: 38-39

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Formation of Gold Nanoparticles by Good Buffers2005

    • Author(s)
      A Habib
    • Journal Title

      Bull.Chem.Jpn 78

      Pages: 262-269

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro Toxicity of Palladium(II) and Gold(III) Porphyrins and their Aqueous Metal Ion Counterparts on Trypanosoma2004

    • Author(s)
      E.Nyarko
    • Journal Title

      Chemic-Biological Interactions,148,(2004). 148

      Pages: 19-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Salting-out Phase Separation of the Mixture of 2-Propanol and Water for Selective Extraction of Cobalt(II) in the Presence of Manganese(III), Nickel, and Copper(II)2004

    • Author(s)
      N.H.Chung
    • Journal Title

      Hydrometallurgy 73

      Pages: 81-89

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fluorescence and Phosphorescence Spectra of Au(III), Pt(II) and Pd(II) Porphyrins with DNA at Room Temperature2004

    • Author(s)
      E.Nyrako
    • Journal Title

      Inorg.Chim.Acta, 357

      Pages: 739-745

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oxidative DNA Damage Induced by HEPES (2-[4-(2-Hydroxyethyl)1-piperazinyl]-ethansufonic acid) Buffer in the Presence of Au(III)2004

    • Author(s)
      A.Habib
    • Journal Title

      J.Inorganic Biochemistry 98

      Pages: 1696-1702

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Propionitrile as an Extraction solvent for the Ion-Pair complexes of Water-Soluble Tetracationic Porphyrinatocopper(II) with Perchlorate : The Effect of sodium Chloride2004

    • Author(s)
      K.Fuji
    • Journal Title

      Bull.Chem.Soc.Jpn. 77

      Pages: 511-517

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Kinetics and Mechanism of Gold(III) Incorporation into Tetrakis(1-methylpyridium-4-yl) porphyrin in Aqueous Solution,2004

    • Author(s)
      A.Habib
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 8

      Pages: 269-1275

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Solvent Composition and Temperature on K+-18-Crpwn-6 Complexation in Acetonitrile-Water Mixed Solvents2004

    • Author(s)
      Yinggung Wu
    • Journal Title

      J.Solution Chemistry 33

      Pages: 777-795

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In vitro Toxicity of Palladium(II) and Gold(III) Porphyrins and their Aqueous Metal Ion Counterparts on Trypanosoma2004

    • Author(s)
      E.Nyarko, T.Hara, D.J.Grab, A.Habib, Y, M.Tabata
    • Journal Title

      Chemic-Biological Interactions 148

      Pages: 19-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Salting-out Phase separation of the Mixture of 2-Propanol and Water for Selective Extraction of Cobalt(II) in the Presence of Manganese(III), Nickel, and Copper(II)2004

    • Author(s)
      N.H.Chung, M.Tabata
    • Journal Title

      Hydrometallurgy 73

      Pages: 81-89

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fluorescence and Phosphorescence Spectra of Au(III), Pt(II) and Pd(II) Porphyrins with DNAat Room Temperature2004

    • Author(s)
      E.Nyrako, N.Hanada, Habib, M.Tabata
    • Journal Title

      Inorg.Chim.Acta 357

      Pages: 739-745

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oxidative DNA Damage Induced by HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethansufonic acid) Buffer in the Presence of Au(III)2004

    • Author(s)
      A.Habib, M.Tabata
    • Journal Title

      J.Inorganic Biochemistry 98

      Pages: 1696-1702

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Propionitrile as an Extraction solvent for the Ion-Pair complexes of Water-Soluble Tetracationic Porphyrinatocopper(II) with Perchlorate : The Effect of sodium Chloride2004

    • Author(s)
      K.Fuji, J.Nishimoto, S.Yamada, M.Fukamachi, M.Tabata
    • Journal Title

      Bull.Chem.Soc.Jpn. 77

      Pages: 511-517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Kinetics and Mechanism of Gold(III) Incorporation into Tetrakis(1-methylpyridium-4-y1)porphyrin in Aqueous Solution2004

    • Author(s)
      A.Hbib, M.Tabata, Y.G.Wu
    • Journal Title

      Journal of Porphyrins and Phthalocyanines 8

      Pages: 1269-1275

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Solvent Composition and Temperature on K+-18-Crpwn-6 Complexation in Acetonitrile-Water Mixed Solvents2004

    • Author(s)
      Yinggung Wu, M.Tabata
    • Journal Title

      J.Solution Chemistry 33(6/7)

      Pages: 777-795

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Solvent Composition and Temperature on K+-18-crown-6 Complexation in Acetonitrile-water Mixed Solvent2004

    • Author(s)
      Y.G.Wu
    • Journal Title

      Journal of Solution Chemistry 33

      Pages: 777-795

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fluoresce and phosphorescence spectra of Au(III),Pt(II)and Pd(II) porphyrins with DNA at room temperature2004

    • Author(s)
      E.Nyarko
    • Journal Title

      Inorg.Chim.Acta 357

      Pages: 739-745

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Oxidative DNA damage induced by HEPES (2-[4-(2-hyrdoxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence of Au(III)2004

    • Author(s)
      A.Habib
    • Journal Title

      J.Inorganic Biochemistry 98

      Pages: 1696-1702

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure of Aqueous Mixtures of N, N,-Dimethylacetamide Studied by Infrared Spectroscopy, X-ray Diffraction, and Mass Spectrometly2003

    • Author(s)
      Toshiyuki Takamuku
    • Journal Title

      J.Phy.Chem..B 107

      Pages: 6070-6078

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Large-Angle X-ray Scattering, Small-Angle Neutron Scattering, and NMR Relaxation Studies on Mixing States of 1,4-Dioxane-Water,1,3-Dioxane-Water, and Tetrahydrofuran-Water Mixtures2003

    • Author(s)
      Toshiyuki Takamuku
    • Journal Title

      J.Molecular Liquds 103-104

      Pages: 143-159

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of the Molecular Properties of Mixed Solvents on the Elution of Alkyl Benzoates in RPLC2003

    • Author(s)
      Midori Yasuda
    • Journal Title

      Anal.Sci. 19

      Pages: 1637-1641

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selective Eextraction of Thallium(III) in the Presence of Gallium(III),Indium(III),Bismuth(III) and Antimony(III) by Salting-out of an Aqueous Mixture of 2-Propanol2003

    • Author(s)
      N.H.Chung
    • Journal Title

      Anal.Chim.Acta 477

      Pages: 243-249

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhanced Conformational Chages in DNA in the Presence of Mercury(II), Cadmium(II) and Lead(II) Porphyrins2003

    • Author(s)
      M.Tabata
    • Journal Title

      Inorg.Biochem. 94

      Pages: 50-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhanced Effects of Porphyrins of Toxic Metal Ions on DNA Conformational Changes, DNA Cleavage and Some Potential Medial Applications2003

    • Author(s)
      M.Tabata
    • Journal Title

      Proceedings of the Silver Jubilee Conference 2003

      Pages: 85-99

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Structure of Aqueous mixtures of N,N-dimethylacetamide studied by infrared spectroscopy, X-ray diffraction, and mass spectrophotometry2003

    • Author(s)
      T.Takamuku, D.Matsuo, M.Tabata, T.Yamaguchi, N.Nishi
    • Journal Title

      J.Phy.Chem.B 107

      Pages: 6070-6078

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Large-angle X-ray scattering, small-angle neutron Scattering, and NMR relaxation studies on mixing states of 1,4-dioxane-water, 1,3-dioxane-water, and tetrahydrofuran-water mixtures2003

    • Author(s)
      T.Takamuku, A.Nakamizo, M.Tabata, K.Yoshida, T.Yamaguchi
    • Journal Title

      J.Molecular Liquds 103-1-4

      Pages: 143-159

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selective extraction of thallium(III) in the presence of gallium(III), indium(III), bismuth(III) and antimony(III) by salting-out of an aqueous mixture of 2-propanol2003

    • Author(s)
      N.H.Chung, J.Nishimoto, O.Kato, M.Tabata
    • Journal Title

      Anal.Chim.Acta 477

      Pages: 243-249

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhanced conformational chages in DNA in the presence of mercury(II), cadmium(II) and lead(II) porphyrins2003

    • Author(s)
      M.Tabata, A.Sarker, E.Nyark
    • Journal Title

      Inorg.Biochem. 94

      Pages: 50-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of the molecular properties of mixed solvents on the elution of alkyl benzoates in RPLC2003

    • Author(s)
      M.Yasuda, T.Sonda, T.Hiraoka, A.Horita, M.Tabata
    • Journal Title

      Anal.Sci. 19

      Pages: 1637-1641

    • NAID

      10011874222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Enhanced Effects of Porphyrins of Toxic Metal Ions on DNA Conformational Changes, DNA Cleavage and Some Potential Medial Applications2003

    • Author(s)
      M.Tabata, E.Nyarko, A.K.Sarkar
    • Journal Title

      Proceedings of the Silver Jubilee Conference 2003,Bangladesh

      Pages: 85-99

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Toshiyuki Takamuku: "Structure of Aqueous Mixtures of N,N,-Dimethylacetamide Studied by Infrared spectroscopy, X-ray Diffraction, and Mass Spectrometry"J.Phys.Chem.B. 107. 6070-6078 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toshiyuki Takamuku: "Large-Angle X-ray Scattering, Small-Angle Neutron Scattering, and NMR Relaxation Studies on mixing States of 1,4-dioxane-Water, 1,3-dioxane-Water, and tetrahydrofuran-Water Mixtures"J.Molecular Liquds. 103-104. 143-159 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Midori Ysuda: "Effects of the Molecular Properties of Mixed Solvents on the elution of Alkyl Benzoates in RPLC"Anal.Scie.. 19. 1637-1641 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kenta Fujii: "Propiononitrile as an extraction solvent for the ion-pair complexes of water-soluble tetracationic porphyrinatocopper(II) with perchlorate : the effect of sodium chloride"Bull.Chem.Soc.Japan. 77. 511-517 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nguyen Huu Chung: "Salting-out phase separation of the mixture of 2-propanol and water for selective extraction of cobalt(II) in the presence of manganese(II), nickel(II), and copper(II)"Hydrometallurgy. 73. 81-89 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toshiyuki Takamuku: "混合溶媒の液体構造と相分離を用いる分析化学"ぶんせき. 2003. 234-240 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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