| Project/Area Number |
15350091
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental chemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
KURASAKI Masaaki Hokkaido Univ., Faculty of Environmental Earth Science, Inst., 大学院・地球環境科学研究院, 助手 (80161727)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAITO Takeshi Hokkaido Univ., School of Medicine, Prof., 医学部保健学科, 教授 (40153811)
HOSOKAWA Toshiyuki Hokkaido Univ., Center of Higher Education, Associate Professor, 高等教育機能開発総合センター, 助教授 (00157025)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2005: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥8,600,000 (Direct Cost: ¥8,600,000)
|
|---|
| Keywords | endocrine disrupters / PC12 cell / apoptosis / bisphenol A / nonylphenol / tributyltin / bax / genetic engineering / Caspase |
|---|
| Research Abstract |
Recently, environmental pollutant including the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetal. In this study, to confirm the system of bioremediation and measurement methods of endocrine disrupters in environmental by using of genetic engineering, we applied the methods of genetic engineering and analytical chemistry.
In this study, on the basis of PC12 cell cultured system which has been known to be induced apoptosis by serum deprivation, we found that tributyl tin inhibited apoptosis and nonylphenol enhanced apoptosis using the cells. These phenomena were considered to be changed the contents of bax and cytochrome C. In addition, we have successful developed convenience measurement system of endocrine disrupters such as dioxins in environment. The detection range of dioxins using the developed assay system showed 1 fmol to 100 pmol.
To construct the system of bioremediation of endocrine disrupters, the genes and cDNAs of enzymes that were expected to degrade the endocrine disrupters, has been cloned. The enzymes expressed in Escherichia coli, showed degraded activity of the endocrine. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining endocrine disrupters in environment.
|
