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Development of bioremediation and of measurement methods of remaining endocrine disrupters in environment.

Research Project

Project/Area Number 15350091
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental chemistry
Research InstitutionHokkaido University

Principal Investigator

KURASAKI Masaaki  Hokkaido Univ., Faculty of Environmental Earth Science, Inst., 大学院・地球環境科学研究院, 助手 (80161727)

Co-Investigator(Kenkyū-buntansha) SAITO Takeshi  Hokkaido Univ., School of Medicine, Prof., 医学部保健学科, 教授 (40153811)
HOSOKAWA Toshiyuki  Hokkaido Univ., Center of Higher Education, Associate Professor, 高等教育機能開発総合センター, 助教授 (00157025)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2005: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordsendocrine disrupters / PC12 cell / apoptosis / bisphenol A / nonylphenol / tributyltin / bax / genetic engineering / Caspase
Research Abstract

Recently, environmental pollutant including the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetal. In this study, to confirm the system of bioremediation and measurement methods of endocrine disrupters in environmental by using of genetic engineering, we applied the methods of genetic engineering and analytical chemistry.
In this study, on the basis of PC12 cell cultured system which has been known to be induced apoptosis by serum deprivation, we found that tributyl tin inhibited apoptosis and nonylphenol enhanced apoptosis using the cells. These phenomena were considered to be changed the contents of bax and cytochrome C. In addition, we have successful developed convenience measurement system of endocrine disrupters such as dioxins in environment. The detection range of dioxins using the developed assay system showed 1 fmol to 100 pmol.
To construct the system of bioremediation of endocrine disrupters, the genes and cDNAs of enzymes that were expected to degrade the endocrine disrupters, has been cloned. The enzymes expressed in Escherichia coli, showed degraded activity of the endocrine. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining endocrine disrupters in environment.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (25 results)

All 2005 2004 2003 Other

All Journal Article (21 results) Publications (4 results)

  • [Journal Article] Metal Binding Ability of Metallothionein-3 expressed in Escherichia coli.2005

    • Author(s)
      Toriumi et al.
    • Journal Title

      Basic & Clinical Pharmacology & Toxicology 96

      Pages: 295-301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Protective effect of dietary azuki bean Vigna angularis seed coats against renal interstitial fibrosis of rats induced by cisplatin.2005

    • Author(s)
      Sato et al.
    • Journal Title

      Nutrition 21

      Pages: 504-511

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] 金投与後のラット腎臓内の酸化的DNA損傷の局在とCpへの影響2005

    • Author(s)
      齋藤 秀 他
    • Journal Title

      Biomedical Research on Trace Elements 16

      Pages: 361-363

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective effect of dietary azuki bean Vigna angularis seed coats against renal interstitial fibrosis of rats induced by cisplatin.2005

    • Author(s)
      Sato, et al.
    • Journal Title

      Nutrition 21

      Pages: 504-511

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Metal Binding Ability of Metallothionein-3 expressed in Escherichia coli.2005

    • Author(s)
      Toriumi, et al.
    • Journal Title

      Basic & Clinical Pharmacology & Toxicology 96

      Pages: 295-301

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Localization and physiological effects of oxidative DNA damages in rat kidney. In Japanese2005

    • Author(s)
      Saito, et al.
    • Journal Title

      Biomedical Research on Trace Elements 16

      Pages: 361-363

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 金投与後のラット腎臓内の酸化的DNA損傷の局在とCpへの影響2005

    • Author(s)
      齋藤秀 他
    • Journal Title

      Biomedical Research on Trace Elements 16

      Pages: 361-363

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Metal Binding Ability of Metallothionein-3 expressed in Escherichia coil2005

    • Author(s)
      Toriumi et al.
    • Journal Title

      Basic & Clinical Pharmacology & Toxicology 96

      Pages: 295-301

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Protective effect of dietary azuki bean Vigna angularis seed coats against renal interstitial fibrosis of rats induced by cisplatin.2005

    • Author(s)
      Sato et al.
    • Journal Title

      Nutrition (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells.2004

    • Author(s)
      Aoki et al.
    • Journal Title

      Life Sciences 74(18)

      Pages: 2301-2312

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synthetic dopamine-melanin, a model for neuromelanin, shows superoxide dismutase like activity.2004

    • Author(s)
      Kimura et al.
    • Journal Title

      Trace Elements and Electrolytes 21

      Pages: 55-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nonylphenol enhances apoptosis induced by serum deprivation in PC 12 cells.2004

    • Author(s)
      Aoki, et al.
    • Journal Title

      Life Sciences 74

      Pages: 2301-2312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Synthetic dopamine-melanin, a model for neuromelanin, shows superoxide dismutase like activity.2004

    • Author(s)
      Kimura, et al.
    • Journal Title

      Trace Elements and Electrolytes 21

      Pages: 55-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical study of macrophage migration inhibitory facfor in rat liver fibrosis induced by thioacetamide2004

    • Author(s)
      Hori et al.
    • Journal Title

      European Journal of Histochemistry 47(4)

      Pages: 317-324

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synthetic dopamine-melanin, a model for neuromelanin, shows superoxide dismutase like activity2004

    • Author(s)
      Kimura et al.
    • Journal Title

      Trace Elements and Electrolytes 21

      Pages: 55-59

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical study of macrophage migration inhibitory factor in rat liver fibrosis induced by thioacetamide.2003

    • Author(s)
      Hori et al.
    • Journal Title

      European Journal of Histochemistry 47(4)

      Pages: 317-324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] メタロチオネインの金属結合能と金属代謝における役割(総説)2003

    • Author(s)
      蔵崎正明他
    • Journal Title

      Food and Food Ingredients Journal of Japan 207

      Pages: 40-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] タウリンの生理作用-肝・腎障害における役割を中心として-(総説)2003

    • Author(s)
      佐藤 伸, 蔵崎正明
    • Journal Title

      Food and Food Ingredients Journal of Japan 208

      Pages: 134-139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical study of macrophage migration inhibitory factor in rat liver fibrosis induced by thioacetamide.2003

    • Author(s)
      Hori, et al.
    • Journal Title

      European Journal of Histochemistry 47

      Pages: 317-324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Roles of metallothionein on metal binding ability and metal metabolism (Review). In Japanese2003

    • Author(s)
      Kurasaki, et al.
    • Journal Title

      Food and Food Ingredients Journal of Japan 207

      Pages: 40-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Physiological roles of taurine on disorders of kidney and liver (Review). In Japanese2003

    • Author(s)
      Sato, Kurasaki
    • Journal Title

      Food and Food Ingredients Journal of Japan 208

      Pages: 133-139

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Aoki et al.: "Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells."Life Sciences. 74(18). 2301-2312 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hori et al.: "Immunohistochemical study of macrophage migration inhibitory factor in rat liver fibrosis induced by thioacetamide."European Journal of Histochemistry. 47(4). 317-324 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimura et al.: "Synthetic dopamine-melanin, a model for neuromelanin, shows superoxide dismutase like activity."Trace Elements and Electrolytes. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 伸: "タウリンの生理作用-肝・腎障害における役割を中心として-(総説)"Food and Food Ingredients Journal of Japan. 208. 134-139 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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