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Structures and Functions of Membrane Antenna Proteins with High Resolution Solution and Solid State NMR

Research Project

Project/Area Number 15350096
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionTohoku University

Principal Investigator

NOZAWA Tsunenori  TOHOKU UNIVERSITY, Graduate School of Engineering, professor, 大学院・工学研究科, 教授 (10006322)

Co-Investigator(Kenkyū-buntansha) WANG Zheng-Yu  TOHOKU UNIVERSITY, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (10213612)
KBAYASHI Masayuki  TOHOKU UNIVERSITY, Graduate School of Engineering, Research Associate, 大学院・工学研究科, 助手 (70271864)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥7,800,000)
KeywordsSolution NMR / Solid state high resolution NMR / Photosynthetic antenna complex / LH1(13870) / Chlorosome antenna / BCh1 a / BCh1 c / B820 / LH1 / BChla / BChlc / 膜タンパク質 / 構造解析 / 光合成膜 / アンテナタンパク質 / 光捕集系錯体1 / 配位構造 / バクテリオクロロフィル / 多次元NMR
Research Abstract

Main object of the research is to establish the method to elucidate structures and functions of membrane proteins and pigment complexes in terms of high resolution solution nuclear magnetic resonance in conjunction with solid state NMR. For the target of the studies we have selected one of the bacterial membrane antenna, and chlorosome anntena. The membrane antenna called LH1 (light harvesting complex 1) or B870 has subunits (B820) composed of two (α and β) polypeptides and two bacteriochlorophyll molecultes in the active site as the prothetic groups. We have established the methods to elucidate the structures around the prothetic group with use of selectively isotope enriched samples. Thus, we reconstituted the subunit B820 of the LH1 with the isotope enrichied BCh1 a and the normal subumits, or the isotope enriched a and the natural β subunit and the natural BChl a and could resolve the structures with use these samples by the solution NMR. In this case the ^<13>C enriched pigment bacteriochlorophylls and the ^2H enriched proteins could focus the signal of the bacteriochlorophylls and the environment. Futhermore we have make extensive use of multidimensional NMR with these samples. These studies have disclosed the method to elucidate membrane proteins in terms of NMR. The whole unit of LH1, i.e., B870 is too large to be investigated by the solution NMR, we have tried to observe the NMR in full use of solid high resolution NMR. The obtained data have been analyzed in terms of the knowledge of the solution NMR. The assigned signal tells us the B870 would be composed of the subunits with similar structures in the solution states. The multi-dimensional solution NMR and the isotope enrichment have afforded the method to clarify the structure of the component a and R polypeptide in the chloroform/methanol solution

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2005 2004 2003 Other

All Journal Article (15 results) Publications (3 results)

  • [Journal Article] Solution Structures of the Core Light-Haravesting αand β Polypeptides from Rhodospirillum rubrum2005

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Journal of Molecular Biology 347

      Pages: 465-477

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Solution Structures of the Core Light-harvesting α and β Polypeptides from Rhodospirillum rubrum2005

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      J.Molecular Biology 347

      Pages: 465-477

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Solution Structures of the Core Light-haravesting α and β Polypeptides from Rhodospirillum rubrum2005

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Journal of Molecular Biology 347

      Pages: 465-477

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Magic-Angle Spinning Nuclear Magnetic Resonance under Ultrahigh Field Reveals Two Forms of Intermolecular Interaction within BChl c Aggregat2004

    • Author(s)
      Mitsuo Umetsu et al.
    • Journal Title

      The Journal of Physical Chemistry B 108(8)

      Pages: 2726-2734

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] NMR Relaxation Study of the Bacteriochlorophyll c in Solutions2004

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      The Journal of Physical Chemistry B 108(39)

      Pages: 15422-15428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Magic-Angle Spinning Nuclear Magnetic Resonance under Ultrahigh Field Reveals Two Forms of Intermolecular Interaction within BChl c Aggregat2004

    • Author(s)
      Mitsuo Umetsu et al.
    • Journal Title

      J.Phys.Chem.B 108(8)

      Pages: 2726-2734

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] NMR relaxation study of bacteriochlorophyll c in solutions2004

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      J.Phys.Chem.B 108(39)

      Pages: 15422-15428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Magic-Angle Spinning Nuclear Magnetic Resonance under Ultrahigh Field Reveals Two Forms of Intermolecular Interaction within BChl c Aggregat2004

    • Author(s)
      Mitsuo Umetsu et al.
    • Journal Title

      The Journal of Physical Chemistry 108(8)

      Pages: 2726-2734

    • Related Report
      2004 Annual Research Report
  • [Journal Article] NMR Relaxation Study of the Bacteriochlorophyll c in Solutions2004

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      The Journal of Physical Chemistry 108(39)

      Pages: 15422-15428

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dynamic Exchange Properties of the Antiparallel BChl c Dimers2003

    • Author(s)
      M.Umetsu et al.
    • Journal Title

      The Journal of Physical Chemistry B 107(36)

      Pages: 9876-9882

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification and characterization of the polypeptides of core light-harvesting complexes from purple sulfur bacteria2003

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Photosynthesis Research 78

      Pages: 133-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Determination of the B820 subunit Size of a Bacterial Core LH Complex by Small-Angle Neutron Scattering2003

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Biochemistry 42(39)

      Pages: 11555-11560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dynamic Exchange Properties of the Antiparallel Bacteriochlorophyll c Dimers2003

    • Author(s)
      Mitsuo Umetsu et al.
    • Journal Title

      J.Phys.Chem.B 107(36)

      Pages: 9876-9882

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Purification and characterization of the polypeptide of core light-harvesting complexes from purple sulfur bacteria2003

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Photosynthesis Research 78

      Pages: 133-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Determination of the B820 Subunit Size of a Bacterial Core Light-Harvesting Complex by Small-Angle Neutron Scattering2003

    • Author(s)
      Zheng-Yu Wang et al.
    • Journal Title

      Biochemistry 42(39)

      Pages: 11555-11560

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] M.Umetsu, et al.: "Dynamic Exchange Properties of the Antiparallel BChl c Dimers"The Journal of Physical Chemistry B. 107(36). 9876-9882 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Z.-Y.Wang, et al.: "Puritication and characterization of the polypeptides of core light-harvesting complexes from purple sulfur bacteria"Photosynthesis Research. 78. 133-141 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Z.-Y.Wang, et al.: "Determination of the B820 Subunit Size of a Bacterial Core LH Complex by Small-Angle Neutron Scattering"Biochemistry. 42(39). 11555-11560 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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