| Project/Area Number |
15360119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dynamics/Control
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| Research Institution | Yamagata University |
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Principal Investigator |
KOSAWADA Tadashi Yamagata University, Mechanical Systems Engineering, Professor, 工学部, 教授 (10143083)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2003: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | Living cell / Dynamic property / Piezo micro sensor / Vibration / Dynamic stimulation / Diagnosis / Human osteoblast / Stress detection / 環境ストレス / 骨芽細胞 / 環境ホルモン / 軟骨細胞 |
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| Research Abstract |
In this study, a novel method to detect the environmental stresses induced in living cell is presented by using dynamic response of piezoelectric vibrator. Experimental studies have shown its capability to detect changes of the environmental stresses naturally or artificially induced in egg cells of killifish as well as cultured human osteoblast. The results can be summarized as follows.
(1) In the egg cell response to Glufosinate (main substance of typical weed killer), with the aid of this sensing system, in the 100ppm case the results are going up just after the Glufosinate injection due to acute toxicity, while 10ppm case the results are going down probably due to "low concentration effect". These phenomena are considered to reflect initial adaptation process of the cell to the surrounding environments.
(2) It is found that surface membrane of the damaged egg cell is harder than the normal cell, while the stimulated egg cell is harder than the damaged cell. Both the damaged cell and the dynamically stimulated cell are considered to be normalized with 48 hours or so. It is also observed that the response to dynamic stimulation depends on frequency.
(3) Experimental method was developed for dynamic stimulation and detection of its response for cultured human osteoblast. It is observed that not only human osteoblast's detection ability against dynamic stimulations but also its information transfer ability to neighboring cells.
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