Analysis of spatio-temporarl mechanisms on outer plexiform layer in retina by the multichannel microprobe electrodes array chip

• Project/Area Number: 15360218
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: RIKEN
• Principal Investigator: USUI Shiro RIKEN, LABORATORY FOR NEUROINFORMATICS, LABORATORY HEAD, ニューロンインフォマティクス技術開発チーム, チームリーダー (40023337)
• Co-Investigator(Kenkyū-buntansha): ISHIDA Makoto TOYOHASHI UNIVERSITY OF TECHNOLOGY, PROFESSOR, 工学部, 教授 (30126924) ; ISHEHARA Akito CHUKYO UNIVERSITY, ASSISTANT PROFESSOR, 生命システム工学部, 講師 (80387620) ; YAMAJI Kazutsuna RIKEN, LABORATORY FOR NEUROINFORMATICS, RESEARCH SCIENTIST, ニューロンインフォマティクス技術開発チーム, 研究員 (50373379) ; 青山 俊弘 独立行政法人理化学研究所, ニューロインフォマティクス技術開発チーム, 研究員 ; 中内 茂樹 豊橋技術科学大学, 工学部, 助教授 (00252320)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥7,900,000 (Direct Cost: ¥7,900,000); Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000); Fiscal Year 2003: ¥5,900,000 (Direct Cost: ¥5,900,000)
• Keywords: retina / multichannel recording / mathematical models / electroretinogram(ERG) / bipolce cell / horizontal cell / microprobe electrode / マルチチャネル記録 / 網膜電位図(ERG) / 光応答計測 / マイクロプローブ

Research Abstract

We have developed multichannel microprobe electrode array chips for recording neural responses to light from retina, and mathematical model of retinal neurons to analyze retinal mechanisms involved in the recorded light-induced responses.
Prototypes of the microprobe electrode array(MEA) chip have been fabricated on Si(111) substrates by selective Vapor-Liquid-Solid(VLS) growth method. The prototype MEA could detect light responses whose characteristics to a white flash and sensitivities to monochromatic stimulus were consistent with photopic electoretinogram(ERG). We also demonstrated that the changes of localized ERG responses were obtained with stimulus of slit light by the MEA.
In order to analyze inner retinal mechanisms involved in the measured ERG responses, we have developed models of retinal neuron and the retinal neural circuit by physiological engineering technique. The models of horizontal cell and bipolar cells which is closely related to form b-wave in ERG responses were described mathematically by their ionic mechanisms. It is allow us to analyze the relationship between light responses and the ionic mechanisms in their neuron.
These results suggest that the developed MEA chip is applicable to retinal neural recordings as a new measurement tool, and the models of retinal neuron would play a role to understand visual information preprocessing of retina.

Research Products

• [Journal Article] Simulation Analysis of Receptive Field Size of Retinal Horizontal Cells by Ionic Current Model (2005)
  • Author(s): Aoyama, T., Kamiyama, Y., Usui, S.
  • Journal Title: Visual Neuroscience 22・1 Pages: 1-14
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] シリコンマイクロプローブ電極アレイチップによる網膜光応答計測 (2005)
  • Author(s): 針本, 石原, 河野, 石田, 臼井
  • Journal Title: 日本生理学会大会 (印刷中)
  • NAID: 130005447564
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Simulation analysis of receptive field size of retinal horizontal cells by ionic current model (2005)
  • Author(s): Aoyama, T., Kamiyama, Y., Usui, S.
  • Journal Title: Visual Neuroscience (accepted for publication)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Recording of light responses in the carp retina using Si microprobe electrode array chip (2005)
  • Author(s): Harimoto, T., Ishihara, A., Kawano, T., Ishida, M., Usui, S.
  • Journal Title: Japanese Journal of Physiology Vol.55
  • NAID: 130005447564
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] マイクロプローブ電極アレイによる網膜光応答計測 (2004)
  • Author(s): 針本, 石原, 河野, 石田, 臼井
  • Journal Title: 電子情報通信学会技術研究報告 103 Pages: 71-76
  • NAID: 110003232532
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] オンライン推定法による網膜神経細胞膜特性の計測 (2004)
  • Author(s): Kamiji, 石原, 藤井, 臼井
  • Journal Title: 電子情報通信学会技術研究報告 103 Pages: 77-82
  • NAID: 110003232533
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Selective Vapor-liquid-solid Epitaxial Growth of Micro-Si Probe Electrode Arrays With On-chip MOSFETs on Si (111) Substrates (2004)
  • Author(s): Kawano, T., Kato, Y., Tani, R., Takao, H., Sawada, K., Ishida M.
  • Journal Title: IEEE Trans.Electron Device 51 Pages: 415-420
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Three-Dimensional Multichannel Si Microprobe Electrode Array Chip for Analysis of the Nervous System (2004)
  • Author(s): Kawano, T., Ishihara, A., Harimoto, T., Takao, H., Sawada, K., Usui, S., Ishida, M.
  • Journal Title: Proceeding of the IEEE International Electron Devices Meeting Pages: 1013-1016
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Recording of light-evoked responses from isolated carp retina using a new Si microprobe electrode array. (2004)
  • Author(s): Harimoto, T., Ishihara, A., Kawano, T., Ishihda, M., Usui, S.
  • Journal Title: Technical Report of IEICE Vol.103 Pages: 71-76
  • NAID: 110003232532
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Measurement of membrane properties of retinal neurons using online estimation method. (2004)
  • Author(s): Kamiji, N.L., Ishihara, A., Fujii, Y., Usui, S.
  • Journal Title: Technical Report of IEICE Vol.103 Pages: 77-82
  • NAID: 110003232533
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selective vapor-liquid-solid epitaxial growth of micro-Si probe electrode arrays with on-chip MOSFETs on Si(111) substrates (2004)
  • Author(s): Kawano, T., Kato, Y., Tani, R., Takao, H., Sawada, K., Ishida, M.
  • Journal Title: IEEE Trans.Electron Device Vol.51 Pages: 415-420
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selective Vapor-liquid-solid Epitaxial Growth of Micro-Si Probe Electrode Arrays With On-chip MOSFETs on Si(111) Substrates (2004)
  • Author(s): Kawano, T., Kato, Y., Tani, R., Takao, H., Sawada, K., Ishida M.
  • Journal Title: IEEE Trans.Electron Device 51 Pages: 415-420
• [Journal Article] The role of ionic currents in shaping light responses of retinal bipolar cells (2003)
  • Author(s): Ishihara, A., Kamiyama, Y., Usui, S.
  • Journal Title: The Neural Basis of Early Vision(Kaneko A.(ed))(Springer-Verlag) Pages: 113-116
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Multichannel 5×5-site 3-dimensional Si micro-probe electrode array for neural activity recording (2003)
  • Author(s): Kawano, T., Takao, H., Sawada, K., Ishida, M.
  • Journal Title: Japanese Journal of Applied Physics Vol.42 Pages: 2473-2477
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Signal conditioning CMOS circuits integrated on Si(111) for image-recording sensor of neural activity (2003)
  • Author(s): Kato, Y., Kawano, T., Ito, Y., Takao, H., Sawada, K., Ishida, M.
  • Journal Title: IEEJ Trans.Sensors and Micromachines Vol.123 Pages: 363-367
  • NAID: 10011752127
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 河北知克, 石原彰人, 臼井支朗, 立花政夫: "網膜双極細胞シナプス終末における伝達物質放出機構の数理モデル"第7回視覚科学フォーラム予稿集. 14 (2003)
• [Publications] Nakauchi S, Ishihara A, Usui S: "Fundamental research on visual information processing"Proceedings of the Second Symposium of Intelligent Human Sensing. 47-50 (2004)
• [Publications] 針本哲弘, 石原彰人, 河野剛士, 石田誠, 臼井支朗: "ヤイクロプローブ電極アレイによる網膜光応答計測"電子情報通信学会技術研究報告. 103・732. 59-64 (2004)
• [Publications] Kawano T, Takao H, Sawada K, Ishida M: "Multichannel 5×5-site 3-dimensional Si micro-probe electrode array for neural activity recording"Journal of Applied Physics. 42. 2473-2477 (2003)
• [Publications] Kawano T, Kato Y, Tani R, Ishino H, Takao H, Sawada K, Ishida M: "Neuron size Si probe array fabricated on integrated circuits for multichannel electrode"Proceedings of The 12th International Conference of Solid-State Sensors and Actuators (Transducers'03). 2. 1679-1682 (2003)