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Magnetic immunoassay system utilizing magnetic marker and SQUID sensor

Research Project

Project/Area Number 15360220
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Measurement engineering
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

ENPUKU Keiji  KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Professor, 大学院・システム情報科学研究院, 教授 (20150493)

Co-Investigator(Kenkyū-buntansha) YOSHINAGA Kohji  Kyushu Institute of Technology, Department of Applied Chemistry, Professor, 工学部, 教授 (00040436)
KISS Takanobu  KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Associate Professor, 大学院・システム情報科学研究院, 助教授 (00221911)
NOZAKI Yukio  KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Research Assistant, 大学院・システム情報科学研究院, 助手 (30304760)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsSQUID sensor / High Tc superconductor / Magnetic nanoparticle / Magnetic relaxation / magnetic marker / Immunoassay / antigen-antibody reaction / 吸着
Research Abstract

Immunoassays are widely used to detect disease-related proteins for medical diagnostics. These proteins are generically called antigens, and the so-called binding reaction between antigen and its antibody is used for the immunoassay. In order to improve the performance of the immunoassay, we have developed a new detection system utilizing a magnetic marker and a SQUID sensor. The main results obtained in this study are as follows.
(1)We clarified the dependence of the magnetic properties on the size of the magnetic nanoparticle. We showed that the particle with nominal diameter of 25 nm can keep remanence, and can generate large magnetic signal. This particle is, therefore, suitable for magnetic immunoassay.
(2)We developed a coating process to cover the magnetic particle with polymer. With this process, we fabricated a polymer-coated magnetic nanoparticle that shows good dispersion in liquid.
(3)We developed the high Tc SQUID system for detecting magnetic signal from the marker. Since the signal decays rapidly in space, we realized a close distance between the cooled SQUID and the room temperature sample.
(4)We developed a magnetic-contamination free reaction chamber and a method to cancel environmental noise in order to detect very weak signal. Our system can detect 12 samples in a short measurement time of a few minutes with a field resolution of about 10 pT.
(5)We conducted a detection of protein called IgE. We demonstrated the detection of IgE down to 0.3 pg, which shows the high sensitivity of the present magnetic method. We also demonstrated the detection of IgE in human serum, which shows the possibility to practical diagnosis.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (43 results)

All 2005 2004 2003 Other

All Journal Article (36 results) Book (1 results) Publications (6 results)

  • [Journal Article] Properties of magnetic nanoparticles for magnetic immunoassays utilizing a superconducting quantum interference device2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn. J. Appl. Phys. vol.44, no.1A

      Pages: 149-155

    • NAID

      10014322007

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetic marker and high T_e superconducting quantum interference device for biological immunoassays2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEICE Trans. Electron. E88-C, no.2

      Pages: 158-167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles2005

    • Author(s)
      T.Tsukamoto et al.
    • Journal Title

      IEEE Trans. Appl. Supercond. vol.15, no.2

      Pages: 656-659

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetic immunoassays utilizing magnetic markers and a high Tc SQUID2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans. Appl. Supercond. vol.15, no.2

      Pages: 660-663

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recent topics in High-Tc superconductive electronics2005

    • Author(s)
      M.Tonouchi et al.
    • Journal Title

      Jpn. J. Appl. Phys.,pp.(2005) vol.44, no.11

      Pages: 7735-7749

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantitative eveluation of magnetic immunoassay with remanence measurement2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Ext. Abst. Int. Superconductive Electronics Conference (ISEC 05)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Properties of magnetic nanoparticles for magnetic immunoassays utilizing a superconducting quantum interference device2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn.J.Appl.Phys. vol.44,no.1A

      Pages: 149-155

    • NAID

      10014322007

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Magnetic marker and high T_c superconducting quantum interference device for biological immunoassays2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEICE Trans.Electron. E88-C,no.2

      Pages: 158-167

    • NAID

      110003215102

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles2005

    • Author(s)
      T.Tsukamoto et al.
    • Journal Title

      IEEE Trans.Appl.Supercond. vol.15,no.2

      Pages: 656-659

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetic immunoassays utilizing magnetic markers and a high Tc SQUID2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans.Appl.Supercond. vol.15,no.2

      Pages: 660-663

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recent topics in High-Tc superconductive electronics2005

    • Author(s)
      M.Tonouchi et al.
    • Journal Title

      Jpn.J.Appl.Phys. vol.44,no.11

      Pages: 7735-7749

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantitative evaluation of magnetic immunoassay with remanence measurement2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Ext.Abst.Int.Superconductive Electronics Conference (ISEC 05) P-H-24-1-2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Magnetic marker and high T_c superconducting quantum interference device for biological immunoassays2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEICE Trans.Electron. E88-C, no.2

      Pages: 158-167

    • NAID

      110003215102

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles2005

    • Author(s)
      T.Tsukamoto et al.
    • Journal Title

      IEEE Trans.Appl.Supercond. vol.15, no.2

      Pages: 656-659

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Magnetic immunoassays utilizing magnetic markers and a high Tc SQUID2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans.Appl.Supercond. vol.15, no.2

      Pages: 660-663

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Magnetometer utilizing SQUID pico-voltmeter and cooled normal pickup coil2005

    • Author(s)
      T.Q.Yang et al.
    • Journal Title

      Physica C vol.C426-431

      Pages: 1596-1600

    • Related Report
      2005 Annual Research Report
  • [Journal Article] SQUIDを用いた先端計測システム2005

    • Author(s)
      円福敬二
    • Journal Title

      固体物理 vol.40, no.10

      Pages: 829-836

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Recent topics in High-Tc Superconductive electronics2005

    • Author(s)
      M.Tonouchi et al.
    • Journal Title

      Jpn.J.Appl.Phys. vol.44, no.11

      Pages: 7735-7749

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Magnetic marker and high Tc superconducting quantum interference device for biological immunoassays2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEICE Trans.Electron. Vol.E88-C No.2

      Pages: 158-167

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Magnetic Immunoassays Utilizing Magnetic Markers and a High-T_c SQUID2005

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans.Appl.Supercond (to be published)(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Application of HTS SQUIDs to biological immunoassays2004

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Physica C vol.412-414

      Pages: 1473-1479

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of magnetic nanoparticles with ac susceptibility measurement2004

    • Author(s)
      T.Q.Yang et al.
    • Journal Title

      Physica C vol.412-414

      Pages: 1496-1500

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Design of superconducting quatum interference device for magnetic immunoassays2004

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn. J. Appl, Phys. vol.43, no.9A

      Pages: 6044-6049

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 磁性ナノ粒子をマーカーにしたSQUID免疫検査システム2004

    • Author(s)
      円福敬二
    • Journal Title

      応用物理 vol.73, no.1

      Pages: 28-31

    • NAID

      10011982906

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 高温超伝導SQUIDを用いた免疫検査システムの開発2004

    • Author(s)
      円福敬二
    • Journal Title

      日本応用磁気学会第137回研究会 資料

      Pages: 33-40

    • NAID

      10013461239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of magnetic nanoparticles with ac susceptibility measurement.2004

    • Author(s)
      T.Q.Yang et al.
    • Journal Title

      Physica C vol.412-141

      Pages: 1496-1500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Design of superconducting quantum interference device for magnetic immunoassays2004

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn.J.Appl.Phys. vol.43,no.9A

      Pages: 6044-6049

    • NAID

      10013573397

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Application of HTS SQUIDs to biological immunoassays2004

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Physica C Vol.412-414

      Pages: 1473-1479

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of magnetic nanoparticles with ac susceptibility measurement2004

    • Author(s)
      T.Q.Yang et al.
    • Journal Title

      Physica C Vol.412-414

      Pages: 1496-1500

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Design of superconducting quantum interference device for magnetic immunoassays2004

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn.J.Appl.Phys. Vol.43No.9A

      Pages: 6044-6049

    • NAID

      10013573397

    • Related Report
      2004 Annual Research Report
  • [Journal Article] High Tc SQUID system and magnetic marker for biological immunoassays2003

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans. Appl. Supercond. vol.13, no.2

      Pages: 371-376

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SQUID magnetometer utilizing normal pickup coil and resonant-type coupling circuit2003

    • Author(s)
      T.Q.Yang et al.
    • Journal Title

      Physica C vol.392-396

      Pages: 1396-1400

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Biological immunoassay utilizing magnetic marker and high Tc superconducting quantum interference device magnetometer2003

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn. J. Appl, Phys. vol.42, no.12A

    • NAID

      130004530263

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SQUIDを用いたバイオ免疫診断2003

    • Author(s)
      円福敬二
    • Journal Title

      低温工学 vol.38, no.9

      Pages: 469-476

    • NAID

      10011371281

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] High Tc SQUID system and magnetic marker for biological immunoassays.2003

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      IEEE Trans.Appl.Supercond. vol.13,no2

      Pages: 371-376

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Biological immunoassays utilizing Magnetic marker and high Tc superconducting quantum interference device magnetometer.2003

    • Author(s)
      K.Enpuku et al.
    • Journal Title

      Jpn.J.Appl.Phys. vol.42,no.12A

    • NAID

      130004530263

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Vortex Electronics and SQUIDs2003

    • Author(s)
      K.Enpuku et al.
    • Total Pages
      302
    • Publisher
      Springer, Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] K.Enpuku et al.: "High Tc SQUID system and magnetic marker for biological immunoassay"IEEE Trans.Appl.Supercond.. Vol.13,No.2. 371-376 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] T.Q.Yang et al.: "SQUED magnetometer utilizing normal pickup coil and resonant-type coupling circuit"Physica C. 392-396. 1396-1400 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 円福 敬二: "SQUIDを用いたバイオ免疫検査"低温工学. Vol.39No.9. 17-24 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 円福 敬二: "磁気ナノ粒子をマーカーにしたSQUID免疫検査システム"応用物理. Vol.73No.1. 28-31 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Enpuku et al.: "Biological immunoassay utilizing magnetic marker and high Tc SQUID"Jpn.J.Appl.Phys.. Vol.42No.12A. L1436-L1438 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Enpuku et al.: "Pplication of HTS SQUIDs to biological immunoassays"Physica C. (to be published). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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