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Isolation of Denitrifying Phosphorus Removing Bacteria and Application of the Biochemical Information for Optimal Control of Advanced Wastewater Treatment Plant

Research Project

Project/Area Number 15360288
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KUBA Takahiro  KYUSHU UNIVERSITY, Faculty of Engineering, Associate Professor, 大学院・工学研究院, 助教授 (60284527)

Co-Investigator(Kenkyū-buntansha) KUSUDA Tetsuya  KYUSHU UNIVERSITY, Faculty of Engineering, Professor, 大学院・工学研究院, 教授 (50037967)
OHISHI Kyoko  KYUSHU UNIVERSITY, Faculty of Engineering, Research Associate, 大学院・工学研究院, 助手 (20110835)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2005: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2003: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsDenitrification / Dephosphatation / DN-PAO / Isolation / Advanced wastewater treatment / Water quality / Acetate / ASM
Research Abstract

A brand-new screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the alternating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron acceptor and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from one condition to another condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, several kinds of bacteria were obtained. Almost isolated bacteria were able to accumulate polyphosphate and PHB under unique conditions such as alternating anaerobic-anoxic conditions. By microscopy with a staining method it was reasonable to suppose qualitatively that these bacteria were responsible for enhanced biological phosphorus removal. However, it was not quantitatively observed that these pure cultures released phosphate with acetate under anaerobic conditions and consumed phosphate under anoxic conditions.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (21 results)

All 2006 2005 2004 2003 Other

All Journal Article (15 results) Publications (6 results)

  • [Journal Article] Effect of Particulate Biodegradable COD in a Post-Denitrification Enhanced Biological Phosphorus Removal System2006

    • Author(s)
      Vladimir TORRICO et al.
    • Journal Title

      Journal of Environmental Science and Health, Part A Toxic/Hazardous Substance & Environmental Eng. A41・8(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of Particulate Biodegradable COD in a Post-Denitrification Enhanced Biological Phosphorus Removal System2006

    • Author(s)
      Vladimir TORRICO et al.
    • Journal Title

      Journal of Environmental Science and Health, Part A Toxic/Hazardous Substance & Environmental Engineering Vol.A41,No.8(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of Particulate Biodegradable COD in a Post-Denitrification Enhanced Biological Phosphorus Removal System.2006

    • Author(s)
      Vladimir TORRICO et al.
    • Journal Title

      Journal of Environmental Science and Health, Part A Toxic/Hazardous Substance & Environmental Engineering A41・8(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of Nutrient Removal Efficiency between Pre-and Post-Denitrification Wastewater Treatment2005

    • Author(s)
      Koji HAMADA et al.
    • Journal Title

      Proceedings of the International Water Association (IWA) Conference 2005,Xi'an

      Pages: 649-656

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activities of Enhanced Biological Nutrient Removal by the UCT Process in Low-Temperature Terms2005

    • Author(s)
      Koji HAMADA et al.
    • Journal Title

      JSCE Journal of Environmental Systems and Engineering Vol.VII-34

      Pages: 783-783

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of Nutrient Removal Efficiency between Pre- and Post-Denitrification Wastewater Treatment2005

    • Author(s)
      Koji HAMADA et al.
    • Journal Title

      Proceedings of the International Water Association (IWA) Conference 2005 Xi'an

      Pages: 649-656

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of Nutrient Removal Efficiency between Pre- and Post-Denitrification Wastewater Treatment2005

    • Author(s)
      Koji HAMADA et al.
    • Journal Title

      Proceedings of the International Water Association (IWA) Conference 2005, Xi'an

      Pages: 649-656

    • Related Report
      2005 Annual Research Report
  • [Journal Article] UCT法を用いた栄養塩除去法の冬季における問題点2005

    • Author(s)
      浜田 康二, et al.
    • Journal Title

      土木学会論文集 VII-34・783

      Pages: 61-69

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 微生物による下水の浄化と水環境の保全2005

    • Author(s)
      久場 隆広, et al.
    • Journal Title

      環境 20

      Pages: 13-16

    • Related Report
      2004 Annual Research Report
  • [Journal Article] FISH法による脱窒性脱リン細菌に対するアプローチ2005

    • Author(s)
      金山 拓広, et al.
    • Journal Title

      日本本水環境学会年会講演概要集 39

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nitrogen and Phosphorus Removal in Two-Sludge System -Volume Exchange Ratio Effect-2005

    • Author(s)
      Toricco C.Vadimir, et al.
    • Journal Title

      日本水環境学会年会講演概要集 39

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Recycling Technology of Water-Biological Sewage Treatment and Prevention of Eutrophication-2004

    • Author(s)
      Takahiro KUBA et al.
    • Journal Title

      Proc.of First International Workshop on Sustainable Habitat Systems-Concept and Technology-

      Pages: 63-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recycling Technology of Water〜Biological Sewage Treatment and Prevention of Eutrophication〜2004

    • Author(s)
      Takahiro Kuba, et al.
    • Journal Title

      First International Workshop on Sustainable Habitat Systems〜Concept and Technology〜

      Pages: 63-68

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 脱窒性脱リン細菌の集積と単離に関する検討2004

    • Author(s)
      肘井 史朗, et al.
    • Journal Title

      第59回土木学会年次学術講演会 Disk 2

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation of the Denitrifying phosphate-Accumulating Organisms Using Alternating Anaerobic-Anoxic Screening Method2003

    • Author(s)
      Koji HAMADA et al.
    • Journal Title

      Environmental Engineering Research Vol.40

      Pages: 53-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] 浜田 康治, et al.: "嫌気-無酸素連続回分スクリーニング法による脱窒脱リン細菌単離の試み"環境工学研究論文集. 40. 53-61 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 金山 拓大, et al.: "活性汚泥からの酢酸資化性リン除去菌の単離"土木学会年次学術講演会 講演講演集. 58・VII(CD-rom). (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 肘井 史朗, et al.: "嫌気-無酸素連続回分スクリーニングにより活性汚泥から得られた単離菌の特性評価"平成15年度土木学会西部支部研究発表会 講演概要集. B-416-B-417 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 浜田 康治, et al.: "脱窒脱リン過程のモデル化に関する検討"日本水環境学会年会 講演概要集. 38(CD-rom). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Torrico C.Vladimir, et al.: "Anoxic Phosphorus Removal in a Post-Denitrification System"日本水環境学会年会 講演概要集. 38(CD-rom). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 岡崎 光夫, et al.: "冬季におけるUCT法でのリン除去低下およびその対策"日本水環境学会年会 講演概要集. 38(CD-rom). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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