| Project/Area Number |
15360437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National University Corporation Tokyo University of Agriculture and Technology |
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Principal Investigator |
TAKEYAMA Haruko National University Corporation Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Professor, 大学院・共生科学技術研究部, 教授 (60262234)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tuyoshi National University Corporation Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Lecturer, 大学院・共生科学技術研究部, 講師 (20345333)
ARAKAKI Atsushi National University Corporation Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Assistant Professor, 大学院・共生科学技術研究部, 助手 (10367154)
MARUYAMA Tadashi Japan Agency for Marine-Earth Science and Technology, Program manager, 極限環境生物圏研究センター, プログラムディレクター (90373464)
大河内 美奈 名古屋大学, 大学院・工学研究科, 講師 (70313301)
大森 信 阿嘉島臨界研究所, 所長(研究職)
谷口 洋基 阿嘉島臨界研究所, 研究員
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2003: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Marine symbiotic consortium / Nano-sized beads / Symbiotic / Associated bacteria / Screening / Marine organisms / Sponge / Coral / Useful materials |
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| Research Abstract |
Recently, marine microbial consortium has been paid attention as new targets for screening of biologically active agents and so on. However, most of bacteria in environment are unculturable and unidentified. In this study, we aimed to have gene resources and develop the micro-assay system for small number of cells which are directly collected. investigate biodiversity, species identification of bacteria associated with marine sponge and coral, which are well known to produce several biologically active agents. Furthermore, methods for their efficient collection from host cells and genome amplification, and micro-screening system have been developed.
Microbial cells were sorted by using flow cytometer and their 16SrDNA analyses were performed before/after cell sorting. 16SrDNA analysis showed that various kinds of bacteria which were not found in the sample not sorted were observed in the sorted cell samples. Those cells are not enough number of cells for construction of genome library. They are subjected to genome amplification by MDA method using phai-29 DNA polymerase. It was confirmed that enough genome DNA was amplified and amplicons less than 10 Kb were not chimeric when the model microbes were used. Furthermore, amine-coated nano-beads showed high performance in DNA extraction/collection.
In order to micro-screening of useful materials, several devises were constructed. Cell-capture devise constructed showed 90% cell capture without any non-specific adsorption. Furthermore, micro-capillary devise for micro-screening was also constructed. In the devise, gradient of chemicals introduced into capillary was observed. This showed that it is possible to examine biological effect of target chemicals under the various of their concentration.
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