| Project/Area Number |
15370004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
ISHIURA Masahiro Nagoya University, Center for Gene research, Professor, 遺伝子実験施設, 教授 (20132730)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Yasuo Nagoya University, Center for Gene research, Associate Professor, 遺伝子実験施設, 助教授 (70154507)
IHARA Kunio Nagoya University, Center for Gene research, Research Associate, 遺伝子実験施設, 助手 (90223297)
九町 健一 名古屋大学, 大学院・生命農学研究科, 学術振興会研究員
NAMBA Keiichi Osaka. University, Graduate School of Frontier Bioscience, Professor, 大学院・生命機能研究科, 教授 (30346142)
KATO Hiroaki Kyoto University, Graduate School of Pharmacology, Professor, 大学院・薬学研究科, 教授 (90204487)
KUCHO Kenich Nagoya University, Graduate School of Agricultural Science, Postdoctoral fellow
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥11,900,000 (Direct Cost: ¥11,900,000)
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| Keywords | Biological clock / Molecular machinery / Cyanobacteria / Clock proteins / Clock genes / Arabidopsis / Bioluminescence / Real-time monitoring / 藍色細菌 / KaiC / 六量体 / リン酸化 / 相互作用 / DNAマイクロアレイ / 発光レポーター遺伝子 / X線結晶構造解析 |
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| Research Abstract |
The cyanobacteria clock machinery is composed of three Kai proteins, KaiA, KaiC, and KaiC. We found that KaiC forms hexmers in the presence of ATP and clarified the three-dimensional structure of KaiC hexamer by electron microscopic observation followed by single particle analysis. We determined the stoichiomertic interactions between KaiA and KaiC. We determined the crystal structure of the C-terminal clock oscillator domain of KaiC and identified His270 as a functional residue. Based on the structure, we demonstrated the structure-function relationships of KaiA. We determined the different roles of the two domains, N-terminal and C-terminal domains, of KaiC.
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