Molecular basis of induction and execution of programmed cell death in plants
| Project/Area Number |
15370018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUKUDA Hiroo The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (10165293)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2003: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Keywords | Zinnia elegans / Brassinosteroid / Programmed cell death / Microarray / Nuclease / Lipase / Protease / Tracheary element / S1ヌクレアーゼ / プロモーター |
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| Research Abstract |
1.Microarray analysis of comprehensive gene expression induced by brassinosteroids (BRs) :
To understand tracheary element (TE) cell death mechanism induced by BRs, I analyzed gene expression induced by BRs comprehensively using microarray. As a result, I revealed that most genes involved in TE cell death are induced by BRs rapidly, suggesting that BRs are an initiator of transcriptional activation of cell death-related genes. I also identified transcription factors showing transient and rapid changes by BRs, which are candidates of regulators of cell death related genes.
2.Regulation mechanism of the expression of cell death-related genes
Using a cell-death related gene, ZCP4 as a marker, I identified a cis-element responsible for TE-specific expression, which is designated Tracheary Element Regulating cis Element (TERE). This element is conserved in promoters not only of cell death-related genes but also of cell wall-related genes. Using yeast two hybrid system, I isolated two factors that bind the TERE sequence, both of which were bZip-type transcription factors.
3.Analysis of nucleases
I performed promoter analysis of all the S1 nuclease gene members in Arabidopsis (BFN1-BFN5). BFN1 was only a S1 nuclease gene that was expressed in developing TEs. In various types of cells going to cell death, different S1 nuclease genes were expressed, suggesting that each S1 nuclease gene is involved in distinctive cell death process. I also identified two candidate genes of calcium-dependent nucleases whose activity increases transiently before TE cell death.
4.Function analysis of a nucleus-locating lipase
I have indicated a Zinnia lipase gene whose mRNA increases in a TE-specific manner. Here I isolated its Arabidopsis ortholog and analyzed the expression and function of the Arabidopsis lipase gene. This gene is expressed preferentially in developing TEs and overproduction of the gene caused abnormal vascular formation, suggesting its involvement in cell death process of TEs.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] Involvement of extracellular dilignols in lignification during tracheary element differentiation of isolated Zinnia mesophyll cells.2005
Author(s)
Tokunaga, N., Sakakibara, N., Umezawa, T., Ito, Y., Fukuda, H., Sato, Y.
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Journal Title
Plant Cell Physiol. 46
Pages: 224-232
NAID
Related Report
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[Journal Article] VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation.2005
Author(s)
Koizumi, K., Naramoto, S., Sawa, S., Yahara, N., Ueda, T., Nakano, A., Sugiyama, M., Fukuda, H.
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Journal Title
Related Report
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[Journal Article] Over-expression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis.2004
Author(s)
Narita, N.N., Moore, S., Horiguchi, G., Kubo, M., Demura, T., Fukuda, H., Goodrich, J., Tsukaya, H.
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Journal Title
Plant J. 38
Pages: 699-713
Related Report
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[Journal Article] A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells.2004
Author(s)
Matsuoka, K., Demura, D., Galis, I., Horiguchi, T., Sasaki, M., Tashiro, G., Fukuda, H.
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Journal Title
Plant Cell Physiol. 45
Pages: 1280-1289
NAID
Related Report
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[Journal Article] Identification and molecular characterization of novel anther-specific genes in Oryza sativa, L. by using cDNA microarray.2004
Author(s)
Endo, M., Tsuchiya, T., Saito, H., Matsubara, H., Hakozaki, H., Masuko, H., Kamada, M., Higashitani, A., Takahashi, H., Fukuda, H., Demura, T., Watanabe, M.
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Journal Title
Genes Genet.Syst. 79
Pages: 213-226
NAID
Related Report
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[Publications] Narita, N.N., Moore, S., Horiguchi, G., Kubo, M., Demura, T., Fukuda, H., Goodrich, J., Tsukaya, H.: "Over-expression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis."Plant J.. (in press). (2004)
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