| Project/Area Number |
15370034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MASUDA Ryuichi Hokkaido Univ., Creative Research Initiative "Sousei", Asso. Prof., 創成科学協同研究機構, 助教授 (80192748)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Tetsuya Hokkaido Univ. Museum, Asso. Prof, 総合博物館, 助教授 (90125279)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2006: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | Ezo sika deer / bottleneck / phylogeograph / molecular phylogeny / mitochondrial DNA / diversity / ancient DNA / archaeology |
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| Research Abstract |
The purposes of the present study are to analyze ancient DNA of sika deer bone remains excavated from archaeological sites in Hokkaido, and to reveal changes of their population structures, phylogeographical history, and mechanisms of diversification, compared with genetic data of the modern populatiuons of sika deer. We identified many haplotypes of mitochondrial DNA (mtDNA) from deer remains of various ages (from the Jomon, Epi-Jomon, and Ainu Culture Periods) and of various areas within Hokkaido. The haplotypes included new ones which had not been found from modern sika deer. From more aged archaeological sites, more new haplotypes were found. Molecular phylogenetic studies including mtDNA of modern Honshu sika deer demonstrated that haplotypes of the Hokkaido sika deer form a common cluster between ancient and modern ones. The results show that genetic diversity in the Hokkaido sika deer population has been reduced since the Jomon Period, and that they have experienced large movement of populations as well as dynamic changes of population sizes such as bottlenecks through environmental changes in past Hokkaido. In addition, we investigated mtDNA of present sika deer and compared the data with those of samples collected ten years ago. As a result, we found the movement of the sika deer populations from eastern to western parts of Hokkaido during the last ten years.
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