Roles for phospholipid asymmetry in cell polarity
| Project/Area Number |
15370081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
TANAKA Kazuma Hokkaido University, Institute for Genetic Medicine, Professor, 遺伝子病制御研究所, 教授 (60188290)
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Konomi (FUJIMURA Konomi) Hokkaido University, Institute for Genetic Medicine, Assistant Professor, 遺伝子病制御研究所, 助教授 (80312354)
YAMAMOTO Takaharu Hokkaido University, Institute for Genetic Medicine, Assistant, 遺伝子病制御研究所, 助手 (80312346)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Cell polarity / Cytoskeleton / Intracellular vesicle transport / Endocytosis / Yeast / Lipid asymmetry / P-type ATPase / Phospholipid translocase / アミノリン脂質トランスロケース / ゴルジ体 |
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| Research Abstract |
Cell polarity is a fundamental cell function to all eukaryotic cells. It is also important in diseases : cancer cells display abnormality in cell polarity. On the other hand, in the plasma membrane in eukaryotic cells, it is known that lipid composition is different between extracellular and intracellular leaflets.
We had identified Cdc50 as a gene that regulates formation of cell polarity in yeast. Recently, we showed that the Cdc50 protein forms a complex with the Drs2 p-type ATPase that translocates phospholipids from the extracellular to intracellular leaflet. We have also shown that both ergosterol and phospholipid asymmetry regulate polarized organization of the actin cytoskeleton, and we have published these results. This year, we have further obtained following results.
(1) We have shown that the Cdc50-Drs2 complex and its analogous complexes regulate the endocytic recycling pathway. In addition, we found that the functions of Cdc50-Drs2 are regulated by the Rab family small GTPase Ypt31/32 and its effector Rcy1 (Tanaka and Kamada).
(2) We have demonstrated that Cdc50-Drs2 is involved in vesicle budding regulated by the Arf1 small GTPase. It seemed that Gga proteins serve as effectors in this function of Arf1 (Tanaka and Yamamoto).
Based on these observations, we propose that phospholipid asymmetry plays an important role in the process of vesicle budding from endosomes. Thus, we have also made steady progress this year.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Septin ring assembly requires concentrated action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae2004
Author(s)
Kadota, J., Yamamoto, T., Yoshiuchi, S., Bi, E., Tanaka, K.
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Journal Title
Mol.Biol.Cell 15
Pages: 5329-5345
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Cdc50p, a protein required for polarized growth, associates with Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae2004
Author(s)
Saito, K., Fujimura-Kamada, K., Furuta, N., Kato, U., Umeda, M., Tanaka, K.
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Journal Title
Mol.Biol.Cell 15
Pages: 3418-3432
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisia2003
Author(s)
Toi, H., Fujimura-Kamada, K., Irie, K., Takai, Y., Todo, S., Tanaka, K.
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Journal Title
Mol.Biol.Cell 14
Pages: 2237-2249
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] The upstream regulator, Rsr1p, and downstream effectors, Gic1p and Gic2p, of the Cdc42p small GTPase coordinately regulate initiation of budding in Saccharomyces Cerevisiae2003
Author(s)
Kawasaki, R, Fujimura-Kamada, K., Toi, H., Kato, H., Tanaka, K.
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Journal Title
Genes Cells 8
Pages: 235-250
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae2003
Author(s)
Misu, K., Fujimura-Kamada, K., Ueda, T., Nakano, A., Katoh, H., Tanaka, K.
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Journal Title
Mol.Biol.Cell 14
Pages: 730-747
Description
「研究成果報告書概要(欧文)」より
Related Report
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