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Moleculargenetic investigation on silica uptake in rice

Research Project

Project/Area Number 15380007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKagawa University

Principal Investigator

ICHII Masahiko  Kagawa University, President, 学長 (50036076)

Co-Investigator(Kenkyū-buntansha) TAKETA Shin  Kagawa University, Faculty of Agriculture, Professor, 農学部, 教授 (40216891)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2003: ¥4,200,000 (Direct Cost: ¥4,200,000)
Keywordsrice / silicon / gene / disease resistance / insect resistance / 根 / 養分 / 吸収 / 病害虫抵抗性 / 養分吸収 / ポジショナルクローニング / 飼料 / 突然変異体 / 消化性
Research Abstract

A mutant with low Si uptake was induced by chemical mutagen treatment. We named the mutant GR1, because it was selected based on the resistance against germanium (Ge), an analogue of silicon (Si) with harmful effect on plants. Molecular mapping showed that the causal gene for low silicon uptake in GR1 was located on the long arm of rice chromosome 2.We determined Si content in the mutant and the wild type by chemical analysis and found that the mutant showed a range of 11-43% of Si content of the wild type in different plant organs studied. Interestingly, the mutant showed an increased level of lignin compared to the wild type; we ascribed this elevated lignin content to "compensation effect" which tends to retain firmness of the plant body. GR1 was more susceptible blast disease and insect attacks. There was no remarkable difference in plant mass productivity upon cultivation in a field, but digestibility of the whole-crop-silage by cows was similar between the mutant and wild type. Thus, the reduced level of Si in the mutant did not result in improvement in digestibility by cows. To know the root parts that contribute to Si uptake, we analyzed various rice root mutants defective in lateral roots or root hairs. Our analysis showed that Si was mainly absorbed through lateral roots and that root hairs did not show significant contribution to Si uptake in rice.

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 2005 2004 2003

All Journal Article (23 results)

  • [Journal Article] Satuated humidity accelerates lateral root development in rice (Orysa sativa L.) seedlings by increasing phloem-based auxin transport.2007

    • Author(s)
      Chhun, T., Y.Uno, S.Taketa, T.Azuma, M.Ichii, T.Okamoto, S.Tsurumi
    • Journal Title

      Journal of Experimental Botany (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Isolation of a novel lateral rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin.2006

    • Author(s)
      Wang, H., S.Taketa, A.Miyao, H.Hirochika, M.Ichii
    • Journal Title

      Plant Science 170

      Pages: 70-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Isolation of a novel lateral rootless mutant in rice (Oryza sativa L.) with reduced sensitivity to auxin,2006

    • Author(s)
      Wang, H., S.Taketa, A.Miyao, H.Hirochika, M.Ichii
    • Journal Title

      Plant Science 170

      Pages: 70-77

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cytokinin inhibits lateral root initiation but stimulates lateral root elongation in rice (Oryza sativa L.)2005

    • Author(s)
      Debi, B.R., Taketa, S., Ichii, M.
    • Journal Title

      Journal of Plant Physiology 162

      Pages: 507-515

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Defects in root development and gravity response in the aem1 mutant of rice are associated with reduced auxin-efflux2005

    • Author(s)
      Debi, B.R., Chhun, T., Taketa, S., Tsurumi, S., Xia, K., Miyao, A., Hirochika, H., Ichii, M.
    • Journal Title

      Journal of Plant Physiology 162

      Pages: 678-685

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of ARM2 in the uptake of indole-3-butyric acid in rice (Oryza sativa L.) roots.2005

    • Author(s)
      Chhun, T., S.Taketa, M.Ichii, S.Tsurumi
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1161-1164

    • NAID

      10016622481

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] イネにおけるTos17でタグされた根の形態に関する突然変異系統の分子遺伝学的解析2005

    • Author(s)
      岩尾(虫鹿)純子, 湯尾崇央, 武田真, 宮尾安藝雄, 廣近洋彦, 一井眞比古
    • Journal Title

      育種学研究 7

      Pages: 171-178

    • NAID

      110004049592

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Defects in root development and gravity response in the aem1 mutant of rice are associated with reduced auxin-efflux.2005

    • Author(s)
      Debi, B.R., Chhun, T., Taketa, S., Tsurumi, S., Xia, K., Miyao, A., Hirochika, H., Ichii, M.
    • Journal Title

      Journal of Plant Physiology 162

      Pages: 678-685

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular genetic analysis of a Tos17-tagged mutant line related to root morphology in rice.2005

    • Author(s)
      Iwao, J., T.Yuo, S.Taketa, A.Miyao, H.Hirohiko, M.Ichii
    • Journal Title

      Breeding Research(in Japanese with English summary) 7

      Pages: 171-178

    • NAID

      110004049592

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of ARM2 in the uptake of indole-3-butyric acid in rice (Oryza sativa L.) roots.2005

    • Author(s)
      Chhun, T., S.Taketa, M.Ichii, S.Tsurumi:
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1161-1164

    • NAID

      10016622481

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イネにおけるTos17でタグされた根の形態に関する突然変異系統の分子遺伝学的解析2005

    • Author(s)
      岩尾(虫鹿)純子, 湯尾崇央, 武田 真, 宮尾安藝雄, 廣近洋彦, 一井眞比古
    • Journal Title

      育種学研究 7

      Pages: 171-178

    • NAID

      110004049592

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.).2004

    • Author(s)
      Yao, S-G., Mushika, J., Taketa, S., Ichii, M.
    • Journal Title

      Plant Science 167

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Different behaviour of indole-3-acetic acid and indole-3-butyric acid in stimulating lateral root development in rice (Oryza sativa L.)2004

    • Author(s)
      Chhun, T., Taketa, S., Tsurumi, S., Ichii, M.
    • Journal Title

      Plant Growth Regulation 43

      Pages: 135-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.).2004

    • Author(s)
      Yao, S-G.Mushika, J., Taketa, S., Ichii, M.
    • Journal Title

      Plant Science 167

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Different behavior of indole-3-acetic acid and indole-3-butyric acid in stimulating lateral root development in rice (Oryza sativa L.)2004

    • Author(s)
      Chhun, T., Taketa, S., Tsurumi, S., Ichii, M.
    • Journal Title

      Plant Growth Regulation 43

      Pages: 135-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Ltr1 of rice (Oryza sativa L.).2003

    • Author(s)
      Chhun, T., Taketa, S., Tsurumi, S., Ichii, M.
    • Journal Title

      Plant Growth Regulation 39

      Pages: 161-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of an abscisic acid-insensitive mutation that affects specifically primary root elongation in rice2003

    • Author(s)
      Yao, S-G., Taketa, S., Ichii, M.
    • Journal Title

      Plant Science 164

      Pages: 971-978

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of a short lateral root mutant in rice (Oryza sativa L.).2003

    • Author(s)
      Debi, B.R., Mushika, J., Taketa, S., Miyao, A., Hirochika, H., Ichii, M.
    • Journal Title

      Plant Science 165

      Pages: 895-903

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lateral root formation in rice (Oryza sativa L.) : differential effects of indole-3-acetic acid and indole-3-butyric acid.2003

    • Author(s)
      Wang, S-C., Taketa, S., Ichii, M., Xu, L., Xia, K., Zhou, X.
    • Journal Title

      Plant Growth Regulation 41

      Pages: 41-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological and physiological characteristics of a root-hairless mutant in rice (Oryza sativa L.).2003

    • Author(s)
      Suzuki, H., Taketa, S., Ichii, M.
    • Journal Title

      Plant and Soil 255

      Pages: 9-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interaction between two auxin-resistant mutants and their effects on lateral root formation in rice (Oryza sativa L.).2003

    • Author(s)
      Chhun, T., Taketa, S., Tsurumi, S., Ichii, M.
    • Journal Title

      Journal of Experimental Botany 54

      Pages: 2701-2708

    • NAID

      120001981762

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of an abscisic acid-insensitive mutation that affects specifically primary root elongation in rice (Oryza sativa L.).2003

    • Author(s)
      Yao, S-G., Taketa, S., Ichii, M.
    • Journal Title

      Plant Science 164

      Pages: 971-978

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lateral root formation in rice (Oryza sativa L.) : differential effects of indole-3-acetic acid and indole-3-butyric acid.2003

    • Author(s)
      Wang, S-C.Taketa, S., Ichii, M., Xu, L., Xia, K., Zhou, X.
    • Journal Title

      Plant Growth Regulation 41

      Pages: 41-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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