| Project/Area Number |
15380018
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Horticulture/Landscape architecture
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
MASUDA Kiyoshi Hokkaido Univ., Grad.School of Agric., Prof., 大学院・農学研究科, 教授 (60157203)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIOTSUKI Akira (KANNO Akira) Tohoku Univ., Grad.School of Life Science, Asso.Prof., 大学院・生命科学研究科, 助教授 (10260449)
HOSHINO Yoichiro Hokkaido Univ., Field Sci.Cent.for Northern Biosphere, Res.Assist., 北方生物圏フィールド科学センター, 助手 (50301875)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2004: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
|
|---|
| Keywords | asparagus / homeotic mutant / modified ABC model / MADS-box gene / Gold Schatz / carpel / 改変ABCモデル |
|---|
| Research Abstract |
Two vegetative lines of 'Gold Schatz' asparagus were introduced to Japan from the East Germany in 1966 and maintained in Hokkaido University through a vegetative propagation. One of these lines developed flowers with small tepals showing green color. Stamens were not found, but floral organs resembling the carpel differentiated at whorl 3 (stamen whorl). These adventitious carpels differentiated ovules including an embryo sac and egg cell. These morphological features suggested that the 'Gold Schatz' line might be a homeotic mutant of floral organogenesis, caused by a functional loss of class-B MADS-box genes.
Southern blot analysis indicated that there was no difference in banding patterns of DNA fragments between wild type and mutant plants, when probed with DNA fragments specific for each of class-B and class-C MADS-box genes. Then, the expression of the MADS-box genes (AOAG1 and AOAG2 for class-C and AODEF, AOGLOA and AOGLOB for class-B genes) in wild type and mutant flowers was examined by Northern blot analysis. As a result, the expression of the B-functional genes were detected only in the wild type or scarcely detected in the mutant flowers. The expression of C-functional gene was detected in flowers from both lines. The hybridization experiments in situ displayd the exclusive expression of AODEF and AOGLOB at the primordia for inner tepals and stamens in wild type flowers. It appeared to remain during the early developmental stage, while the expression of AODEF in the mutant flowers rapidly disappeared in the early developmental stage. The transcripts for AOGLOA were not detected in the mutant flowers throughout the development.
These results suggest that the lowered expression of AOGLOA causes a decline of the expression of AODEF and AOGLOB genes, and that the inability of B function genes leads to the phenotype found in an asparagus 'Gold Schatz' homeotic mutant.
|
