| Project/Area Number |
15380030
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | National Agriculture and Bio-oriented Research Organization |
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Principal Investigator |
ICHIMURA Kazuo National Institute of Floricultural Science, Department of Production, Head of Laboratory, 花き研究所生産利用部流通技術研究室, 室長 (20355712)
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| Co-Investigator(Kenkyū-buntansha) |
TANASE Koji National Institute of Floricultural Science, Department of Production, Senior researcher, 花き研究所生産利用部流通技術研究室, 主任研究官 (30355713)
NAKAYAMA Masayoshi National Institute of Floricultural Science, Department of Genetics and Physiology, Head of Laboratory, 花き研究所生産利用部流通技術研究室, 室長 (40237475)
KOHATA Katsunori National Institute of Vegetable and Tea Science, Department of Physiology and Quality Science, Director, 野菜茶業研究所機能解析部, 部長 (70355618)
YAMADA Kunio Nagoya University, Graduate School of Bioagricultural Science, Assistant Professor, 大学院・生命農学研究科園芸科学分野, 助手 (30345871)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥10,300,000 (Direct Cost: ¥10,300,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | flower opening / cell expansion / vacuole / carbohydrate / osmoticum / apoplast / rose / subcellular distribution / トルコギキョウ / デルフィニウム / 糖質抽出法 / 細胞内局在性 / リンドウ / フロックス / ゲンチオビオース / 2-C-メチルエリトリトール / 開花 / キンギョソウ / マンニトール |
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| Research Abstract |
During flower opening, large amount of soluble carbohydrates accumulates, suggesting that soluble carbohydrates play important roles as osmotica in plant cells. In the present study, carbohydrate concentration in cytoplasm, vacuole and apoplast in rose petals was determined and compared with total osmotic potential and osmotic potential derived from carbohydrates. A method for the evaluation of subcellular carbohydrate distributions in petals was developed by combining nonaqueous fractionation method and infiltration-centrifuged method. The volumes of subcellular compartments of petal cells were determined using electron microscope observations. Glucose and fructose concentrations in cytoplasm, vacuole and apoplast were drastically increased with petal opening. With petal opening, total osmotic potentials in symplastic and apoplastic solutions increased. In comparing total osmotic potential with osmotic potential derived from carbohydrates, increase in total osmotic potential with petal opening was mainly attributed to increase in osmotic potential derived from carbohydrates. Thus, accumulation of carbohydrates in the vacuole appears to contribute to cell expansion, leading to the petal opening. To clarify whether carbohydrates accumulate in the vacuole, carbohydrate distributions in cytoplasm, vacuole and apoplast of snapdragon, delphinium, Eustoma grandiflorum, Phlox drummondii, Gentiana triflora var.japonica flowers were investigated. Glucose and fructose in snapdragon, glucose, fructose and mannitol in delphinium, sucrose and glucose in E.grandiflorum, glucose and 2-C-methyl erythritol in P.durmmondii, gentiobiose and sucrose in G.triflora var.japonica increased with petal opening. These carbohydrates distributed mostly in vacuole. In these flowers, accumulation of different carbohydrates depended on species in the vacuole appears to contribute to cell expansion, leading to the flower opening.
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