| Project/Area Number |
15380051
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant nutrition/Soil science
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KIMURA Makoto Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (20092190)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ASAKAWA Susumu Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (50335014)
MURASE Jun Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (30285241)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
|
|---|
| Keywords | Paddy soil ecosystem / PCR-DGGE / Bacteria / Eukaryote / Methanogenic archaea / Methanotroph / Phylogenetic analysis / DNA sequence / メタン生成菌 / 16S rDNA / 水田作土 / RNA / 群集構造 / メタン生成古細菌 / 18s rDNA / 水田 / 稲わら / 稲わら堆肥 / 水稲根 / 細菌群集 / Proteobacteria / CFBグループ / 多様性 / Clostridium / DGGE |
|---|
| Research Abstract |
For elucidating the community structure of microorganisms in the paddy field ecosystem, DNA and RNA were extracted from the samples collected from various habitats such as floodwater, rice straw on and in the plow layer, and surface oxidized and reduced soil layers, and they were PCR-amplified with primers specific to eubacteria, eukaryotes, methanogens, methanotrophs, and ammonia oxidizers. PCR products were subjected to denaturing gradient gel electrophoresis (DGGE) to estimate the seasonal variations and the specificity of respective habitats with the subsequent determination of DNA sequence for characteristic DGGE bands.
In general, microbial communities estimated from PCR-DGGE patterns were phylogenetically endemic at respective habitats with relatively small seasonal variations. Sequence analysis of characteristic DGGE bands at each habitat indicated the presence of endemic microorganisms representing the community, and the microbial habitats were grouped into floodwater and percolating water (aquatic habitat), rice straw, rice straw compost and plant debris (plant residue habitat), rice roots and microcrustaceans (symbiotic habitat).
As eubacterial and methanogenic communities in the plow layer soil that were observed in DGGE analysis of extracted DNA were also detected in DGGE analysis of extracted RNA, all the eubacteria and methanogens were estimated to be active all the year round.
Interesting was the finding that microbial communities estimated from the PCR-DGGE analysis of environmental DNA was similar to those estimated from phospholipid fatty acid (PLFA) analysis.
|
