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Study on the mechanism of Is uptake and translocation in rice

Research Project

Project/Area Number 15380053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOkayama University (2005)
Kagawa University (2003-2004)

Principal Investigator

MA Jian Feng  Okayama University, Res.Inst.Bioresources, Professor, 資源生物科学研究所, 教授 (80260389)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥8,300,000 (Direct Cost: ¥8,300,000)
KeywordsRice / Silicon / transporter / translocation / form / uptake / gene / 突然変異体 / 変異体 / ケイ酸 / クローニング / 輸送形態 / 導管
Research Abstract

1)The form of Si in xylem sap was identified by ^<29>Si-nuclear magnetic resonance (NMR) spectroscopy. In rice (cv.Oochikara) cultured in a monosilicic acid solution containing 0.5mM Si, the Si concentration in the xylem reached 6mM within 30 min. In the ^<29>Si-NMR spectra of the xylem sap, only one signal was observed at a chemical shift of -72.6 ppm, which is consistent with that of monosilicic acid. An ^1H-NMR study of xylem sap did not show any significant difference between the wild-type rice and mutant rice defective in Si uptake, and the ingredient of the xylem sap was not affected by the Si supply. The Si concentration in the xylem sap in vitro decreased from an initial 18 mM to 2.6 mM with time. Addition of xylem sap to a solution containing 8 mM Si did not prevent the polymerization of silicic acid. All these results indicate that Si is translocated in the form of monosilicic acid through the xylem and that the concentration of monosilicic acid is high in the xylem only transiently.
2)A gene, Low silicon rice 1 (Lsi1), controlling silicon accumulation in rice, a typical silicon-accumulating plant, was cloned. This gene belongs to the aquaporin family and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both the exodermis and endodermis cells, where Casperian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only.
3)A novel mutant defective in Si uptake was isolated. This mutant showed lower Si uptake compared to the wild type, but there is no any difference in the uptake of other minerals such as N,P, and K. Mapping result showed that the gene responsible for the mutation is located on the chromosome 3.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004 Other

All Journal Article (17 results) Patent(Industrial Property Rights) (1 results) Publications (1 results)

  • [Journal Article] A silicon transporter in rice2006

    • Author(s)
      Ma, J.F.et al.
    • Journal Title

      Nature 440

      Pages: 688-691

    • NAID

      40015676750

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Silicon transporter in rice.2006

    • Author(s)
      Ma, J.F., Tamai, K., Yamaji, N.Mitani, M., Konishi, S., Katsuhara, M., Ishiguro, M., Murata, Y., Yano, M.
    • Journal Title

      Nature 440

      Pages: 688-691

    • NAID

      40015676750

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A silicon transporter in rice2006

    • Author(s)
      Ma, J.F., Tamai, K., Yamaji, N., Mitani, N., Konishi, S., Katsuhara, M., Ishiguro, M., Murata, Y., Yano, M
    • Journal Title

      Nature 440

      Pages: 688-691

    • NAID

      40015676750

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Plant root responses to three abundant soil mineral : silicon, aluminum and iron2005

    • Author(s)
      Ma, J. F.
    • Journal Title

      Crit. Rev. Plant Sci. 24

      Pages: 267-281

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of silicon form in the xylem of rice (Oryza sativg L.)2005

    • Author(s)
      Mitani, M., Ma, J.F., Iwashita.T.
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 279-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Uptake system of silicon in different plant species2005

    • Author(s)
      Mitani, N., Ma, J.F.
    • Journal Title

      J. Exp. Bot. 56

      Pages: 1255-1261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Plant root responses to three abundant soil mineral : silicon, aluminum and iron.2005

    • Author(s)
      Ma, J.F.
    • Journal Title

      Crit.Rev.Plant Sci. 24

      Pages: 267-281

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of silicon form in the xylem of rice (Oryza sativa L.).2005

    • Author(s)
      Mitani, M., Ma, J.F., Iwashita, T.
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 279-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Uptake system of silicon in different plant species.2005

    • Author(s)
      Mitani, N., Ma, J.F.
    • Journal Title

      J.Exp.Bot. 56

      Pages: 1255-1261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Uptake system of silicon in different plant species2005

    • Author(s)
      Mitani, N., Ma, J.F.
    • Journal Title

      Journal of Experimental Botany 56

      Pages: 1255-1261

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Plant root responses to three abundant soil minerals : silicon, aluminum and iron2005

    • Author(s)
      Ma, J.F.
    • Journal Title

      Critical Reviews in Plant Sciences 24

      Pages: 267-281

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of silicon form in the xylem of rice (Oryza sativa L.)2005

    • Author(s)
      Mitani, M., Ma, J.F., Iwashita, T
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 279-283

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses.2004

    • Author(s)
      Ma, J. F.
    • Journal Title

      Soil Sci. Plant Nutr. 50

      Pages: 11-18

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of Si uptake system and molecular mapping of Si transporter gene in rice2004

    • Author(s)
      Ma, J.F.et al.
    • Journal Title

      Plant Physiol. 136

      Pages: 3284-3289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses.2004

    • Author(s)
      Ma, J.F.
    • Journal Title

      Soil Sci.Plant Nutr. 50

      Pages: 11-18

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of Si uptake system and molecular mapping of Si transporter gene in rice.2004

    • Author(s)
      Ma, J.F.Mitani, M.Nagao, S., Konishi, S., Tamai, K., Iwashita, T., Yano.M.
    • Journal Title

      Plant Physiol. 136

      Pages: 3284-3289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of Si uptake system and molecular mapping of Si transporter gene in rice2004

    • Author(s)
      Ma, J.F., Mitani, M., Nagao, S., Konishi, S., Tamai, K., Iwashita, T., Yano.M
    • Journal Title

      Plant Physiol. 136

      Pages: 3284-3289

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 特許2004

    • Inventor(s)
      馬 建鋒
    • Industrial Property Rights Holder
      香川大学
    • Industrial Property Number
      2004-381899
    • Filing Date
      2004-12-28
    • Related Report
      2004 Annual Research Report
  • [Publications] Ma, J.F.: "Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses"Soil Sci.Plant Nutr.. 50. 11-18 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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