• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Bacterial Two-component and Hetero-heptameric Pore-forming Cytolytic Toxins : Structures, Pore-forming Mechanism

Research Project

Project/Area Number 15380054
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionTohoku University

Principal Investigator

KAMIO Yoshiyuki  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (00109175)

Co-Investigator(Kenkyū-buntansha) KANEKO Jun  Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院・農学研究科, 助手 (30221188)
HIGUCHI Hideo  Tohoku University, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (90165093)
冨田 敏夫  東北大学, 大学院・農学研究科, 助教授 (00126129)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 2004: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2003: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsStaphylococcus aureus / γ-hemolysin / two-component hemolytic toxin / Pore-foming mechanism / Recognition of target cells / Staphylococcus aureus / leukocidin / γ-hemolysin / 膜孔形成 / 一分子技術
Research Abstract

Staphylococcal γ-hemolysin (Hlg), leukocidin (Luk), and Panton-Valentine leukocidin (PVL) are two-component and hetero-oligomeric pore-forming cytolytic toxins (or cytolysin), that were first identified in bacteria. No information on the existence of hetero-oligomeric pore-forming cytolytic toxins in bacteria except for staphylococcal strains is available so far. Hlg (Hlg1 of 34 kDa/Hlg2 of 32 kDa) effectively lyses erythrocytes from human and other mammalian species. Luk (LukF of 34 kDa/LukS of 33 kDa) is cytolytic toward human and rabbit polymorphonuclear leukocytes and rabbit erythrocytes, and PVL (LukF-PV of 34 kDa/LukS-PV of 33 kDa) reveals cytolytic activity with a high cell specificity to leukocytes. Hlg1 is identical to LukF and that the cell specificities of the cytolysins are determined by Hlg2 and LukS. Based on the primary and 3-dimensional structures of the toxin components, Hlg, Luk, and PVL are thought. to form a family of proteins.
Recently, the assembly mechanism of the LukF and Hlg2 monomers into pore-forming hetero-oligomers of Hlg on human erythrocyte membranes has been clarified for the first time by our study using a single-molecular fluorescence imaging technique. We estimated 11 sequential equilibrium constants for the assembly pathway which includes the beginning with membrane binding of monomers, proceeds through single pore oligomerization, and culminates in the formation of clusters of the pores.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (14 results)

All 2005 2004 2003 Other

All Journal Article (12 results) Publications (2 results)

  • [Journal Article] The association between Staphylococcus aureus Strains Carrying Panton-Valentine Leukocidin Genes and the Development of Deep-Seated Follicular Infection PVL Gene-Positive Furuncles2005

    • Author(s)
      O.Yamasaki, J.Kaneko, S.Morizane, H.Akiyama, J.Arata, S.Narita, J.Chiba, Y.Kamio, K.Iwasaki
    • Journal Title

      Clin.Infect.Dis. 40

      Pages: 381-385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Staphylococcal infection by a PVL-converting phage, φSLT starts with molecular interaction between structural protein ORF636 of the phage and Staphylococcal, cell surface via lipoteichoic acid2005

    • Author(s)
      S.Narita, J.Kaneko, Y.Kamio
    • Journal Title

      FEMS microbial lett (submitted)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The association between Staphylococcus aureus Strains Carrying Panton-Valentine Leukocidin Genes and the Development of Deep-Seated Follicular Infection PVL Gene Positive Furuncles.2005

    • Author(s)
      O.Yamasaki, J.Kaneko, S.Morizane, H.Akiyama, J.Arata, S.Narita, J.Chiba, Y.Kamio, K.Iwatsuki.
    • Journal Title

      Clin.Infect.Dis. 40

      Pages: 381-385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Staphylococcal infection by a PVL-converting phage, φSLT starts with molecular interaction between structural protein ORF636 of the phage and Staphylococcal cell surface via lipoteichoic acid.2005

    • Author(s)
      S.Narita, J.Kaneko, Y.Kamio.
    • Journal Title

      FEMS microbial lett. (submitted)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bacterial Two-component and Hetero-heptameric Pore-forming Cytolytic Toxins : Structures, Pore-forming Mechanism, and Organization of the Genes2004

    • Author(s)
      Y.Kamio, J.Kaneko
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(5)

      Pages: 981-1003

    • NAID

      10013144315

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Essential Residues, W177 and R198, of LukF for Phosphatidylcholine-Binding and Pore-Formation by Staphylococcal g-Hemolysin on Human Erythrocyte2004

    • Author(s)
      N.Monma, V.T.Nguyen, J.Kaneko, H.Higuchi, Y.Kamio
    • Journal Title

      J.Biochem 136

      Pages: 427-431

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Cooperative Assembly of b-Barrel pore-Forming Toxins2004

    • Author(s)
      V.T.Nguyen, Y.Kamio
    • Journal Title

      J.Biochem 136

      Pages: 536-567

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Bacterial two-component and hetero-heptameric pore-forming cytolytic toxins : Structures, Pore-forming mechanism, and organization of the genes.2004

    • Author(s)
      J.Kankeo, Y.Kamio.
    • Journal Title

      Biosci.Biotechnol.Biochem. 68(5)

      Pages: 981-1003

    • NAID

      10013144315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Essential residues, W177 and R198, of LukF for phosphatidylcholinebinding and pore-formation by Staphylococcal gamma-hemolysin on human erythrocyte membranes.2004

    • Author(s)
      N.Monma, V.T.Nguyen, J.Kaneko, H.Higuchi, Y.Kamio.
    • Journal Title

      J.Biochem. 136

      Pages: 427-431

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cooperative assembly of beta-barrel pore-forming toxins.2004

    • Author(s)
      V.T.Nguyen, Y.Kamio.
    • Journal Title

      J.Biochem. 136

      Pages: 563-567

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Single-molecule imaging of cooperative assembly of γ-hemolysin on erythrocyte membranes.2003

    • Author(s)
      V.T.Nguyen, Y.Kamio, H.Higuchi
    • Journal Title

      The EMBO Journal 22(19)

      Pages: 4968-4979

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Single-molecule imaging of cooperative assembly of γ-hemolysin on erythrocyte membranes.2003

    • Author(s)
      V.T.Nguyen, Y.Kamio, H.Higuchi.
    • Journal Title

      The EMBO Journal 22(19)

      Pages: 4968-4979

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] V.T.Nguyen, Y.Kamio, H.Higuchi: "Single-molecule imaging of cooperative assembly of γ-hemolysin on erythrocyte membranes"The EMBO Journal. 22(19). 4968-4979 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Y.Kamio, J.Kaneko: "Bacterial Two-component and Hetero-heptameric Pore-forming Cytolytic Toxins : Structures. Pore-forming Mechanism and Organization of the Genes"Biosci.Biotechnol.Biochem.. 67(5). (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi