| Project/Area Number |
15380063
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Osaka University |
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Principal Investigator |
NIHIRA Takuya Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (70144441)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Tatsuji Osaka University, International Center for Biotechnology, Professor, 生物工学国際交流センター, 教授 (50029245)
KINOSHITA Hiroshi Osaka University, International Center for Biotechnology, Assistant professor, 生物工学国際交流センター, 助手 (20294035)
KAWASAKI Hiroko Osaka University, International Center for Biotechnology, Assistant professor, 生物工学国際交流センター, 助手 (30251482)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | Streptomyces / Secondary metabolism / Signaling molecules / Streptomyces / ブチロラクトンオートレギュレーター / レセプター / 破壊株 / virginiamycin生産 / 破壊珠 |
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| Research Abstract |
Streptomyces species are gram-positive soil-dwelling filamentous bacteria that produce a wide variety of antibiotic and other secondary metabolites with important applications in human medicine and agriculture. These secondary metabolites are products of complex biosynthetic pathways including regulatory genes as well as their biosynthetic gene cluster. The activation of antibiotic production has been known to be controlled by the γ-butyrolactone autoregulator, a low-molecular-weight signaling molecule. Although the autoregulator-mediated mechanism has been partially clarified in many Streptomyces species, common trait has still remained unclear. In this research, we focused on framework for autoregulator-mediated mechanism by assembling information from each analysis. In Streptomyces virginiae, the autoregulator (VB) controls virginiamycin production by means of VB binding to VB receptor (BarA). Our analysis on BarB and BarZ, which possess moderate similarity to BarA, showed that virginiamycin production requires function of BarB and BarZ at initial or stationary phase, respectively. barB transcription is directly controlled by BarA binding to barB promoter region. In S.coelicolor A3(2), the model strain of Streptomyces species, autoregulator receptor was found to regulate directly transcriptional regulator, and thus controls expression of biosynthetic genes concerned with secondary metabolism. Moreover, we obtained 4 more autoregulator receptors from industrially important Streptomyces and non-Streptomyces strains. Taken together with these results, we conclude that useful information was successfully accumulated for industrial application.
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