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Molecular analysis of plant Na and K transporter involved in response to environmental stress

Research Project

Project/Area Number 15380070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionNagoya University

Principal Investigator

UOZUMI Nobuyuki  Nagoya University, Bioscience and Biotechnology Center, Professor, 生物機能開発利用研究センター, 教授 (40223515)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2003: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsNa transporter / K transporter / K channel / AtHKT1 / HKT / Ktr / Arabidopsis / cyanobacteria / EF-hand / K^+ / らん藻 / KUP / スフェロプラスト / 大腸菌 / プロトン駆動力
Research Abstract

1 The Ktr-system in Synechocystis sp.PCC 6803 was found to be Na-activated K transporter. The K transport activity was inhibited by CCCP and was not found in the mutant strain which has a mutation of the respiratory chain system. These indicate that proton motive force is involved in the Ktr-mediated K transport. Hyperosmotic stress led initially to loss of net K from the cells. The ktrB mutant shocked with sorbitol failed to reaccumulate K up to its original level. The K uptake via KtrABE palys a crucial role in the early phase of cell turgor regulation after hyperosmotic shock.
2 Bacterial Ktr system and plant HKT-system has eight transmembrane topology. The positive residue in the eighth transmembrane segment was replaced with other amino acids. The conversion from Arg to Lys sustained the K uptake activity but that to negative or non-polar amino acids gave loss of the function. The positive residue is important for the activity.
3 KUP-type transporter was not inserted into the rough microsomal membrane. Then the transporter was expressed in E.coli and their membrane structure was determined.
4 Some of plant K channels has the putative Ca binding sites. The mutation in the region inhibited the K uptake activity. The region is important for the transport activity.
5 Arabidopsis AtHKT1 transporter was expressed in the vascular tissuels in the aerial part of the plant. The null mutant showed that Na accumulated in the xylem tissue and Na was not supplied into the phloem tissue. AtHKT1 is one of the components for Na recirculation of plant.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (23 results)

All 2004 2003 Other

All Journal Article (16 results) Book (1 results) Publications (6 results)

  • [Journal Article] Mechanosensitivity of GIRK channels is mediated by PKC-dependent channel-PIP_2 interaction.2004

    • Author(s)
      Zhang, L., Lee, J-K., John, S.A., Uozumi, N., Kadama, I.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 7037-7047

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Na^+-dependent K^+ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC6803 and its role in the early phases of cell adaptation to hyperosmotic shock.2004

    • Author(s)
      Matsuda N, Kobayashi H, Katoh H, Ogawa T, Futatsugi L, Nakamura T, Bakker EP, Uozumi N.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 54952-54962

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of potassium channels from Arabidopsis thaliana2004

    • Author(s)
      Ibuki T., Tokida Y., Matsuda N., Czempinski K., Muller-Roeber B., Ona T., Uozumi N.
    • Journal Title

      Improvement of forest resources for recyclable forest products

      Pages: 167-169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Large-scale production of hairy root2004

    • Author(s)
      Uozumi N.
    • Journal Title

      Recent progress of biochemical and biomedical engineering in Japan 2

      Pages: 75-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Na^+-dependent K^+ uptake Ktr system from the cyanobacterium Synechocystis sp.PCC6803 and its role in the early phases of cell adaptation to hyperosmotic shock.2004

    • Author(s)
      Matsuda N, Kobayashi H, Katoh H, Ogawa T, Futatsugi L, Nakamura T, Bakker EP, Uozumi N.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 54952-54962

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of potassium channels from Arabidopsis thaliana.2004

    • Author(s)
      Ibuki T., Tokida Y., Matsuda N., Czempinski K., Muller-Roeber B., Ona T., Uozumi N.
    • Journal Title

      Improvement of forest resources for recyclable forest products

      Pages: 167-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Recent progress of biochemical and biomedical engineering in Japan.2004

    • Author(s)
      Uozumi N.
    • Journal Title

      Recent progress of biochemical and biomedical engineering in Japan II.

      Pages: 75-105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mechanosensitivity of GIRK channels is mediated by protein kinase C-dependent channel-phosphatidylinositol 4,5-bisphosphate interaction.2004

    • Author(s)
      Zhang, L., Lee, J-K., John, S.A., Uozumi, N., Kadama, I.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 7037-7047

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Na^+-dependent K^+ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC 6803 and its role in the early phases of cell adaptation to hyperosmotic shock.2004

    • Author(s)
      Mtsuda, N., Kobayashi, H., Katoh, H., Ogawa, T., Futatsugi, L., Nakamura, T., Bakker, E.P., Uozumi, N.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 54952-54962

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of potassium channels from Arabidopsis thaliana2004

    • Author(s)
      Ibuki T., Tokida Y., Matsuda N., Czempinski K., Muller-Roeber B., Ona T., Uozumi N.
    • Journal Title

      Improvement of forest for recyclable forest products

      Pages: 167-169

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional analysis of AtHKT1 in Arabidopsis shows that Na^+ recirculation by the phloem is crucial for salt tolerance2003

    • Author(s)
      Berthomieu, P., ら17名(Uozumi, N.は6番目)
    • Journal Title

      EMBO J. 22

      Pages: 2004-2014

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Topogenesis of two transmembrane type K^+ channels, Kir 2.1 and KcsA2003

    • Author(s)
      Umigai N, Sato Y, Mizutani A, Utsumi T, Sakaguchi M, Uozumi N
    • Journal Title

      J.Biol.Chem. 278

      Pages: 40373-40384

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Addition of a peptide tag at the C terminus of AtHKT1 inhibits its Na^+ transport.2003

    • Author(s)
      Kato Y., Hazama A., Yamagami M., Uozumi N.
    • Journal Title

      Biosci.Biotech.Biochem. 67

      Pages: 2291-2293

    • NAID

      110002698778

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional analysis of AtHKT1 in Arabidopsis shows that Na^+ recirculation by the phloem is crucial for salt tolerance.2003

    • Author(s)
      Berthomieu, P., Conejero G, Nublat A., Brackenbury W.L.Lambert C., Savio, C., Uozumi, N., Oiki, S., Yamada, K., Cellier, F., Gosti, F., Simonneau, T., Essah, P.A., Tester, M., Very, A-A, Sentenac, H., Casse, F.
    • Journal Title

      EMBO J. 22

      Pages: 2004-2014

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Topogenesis of two transmembrane type K^+ channels, Kir 2.1 and KcsA.2003

    • Author(s)
      Umigai N, Sato Y, Mizutani A, Utsumi T, Sakaguchi M, Uozumi N.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 40373-40384

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Metabolic communication in plant : Development of plant molecular physiology2003

    • Author(s)
      Sato Y, Mizutani A, Uozumi N.
    • Journal Title

      Protein, nucleous and enzyme 48

      Pages: 2052-2060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 植物の代謝コミュニケーション 植物分子生理学の新展開 蛋白質核酸酵素増刊号482003

    • Author(s)
      佐藤陽子, 水谷昭文, 魚住信之
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 佐藤陽子, 水谷昭文, 魚住信之: "K^+イオン輸送系の植物成長と塩感受性への役割"蛋白質核酸酵素増刊号 共立出版. 48・15. 2052-2060 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Berthomieu, P., et al.: "Functional analysis of AtHKTl in Arabidopsis shows that Na^+ recirculation by the phloem is crucial for salt tolerance"EMBO J.. 22. 2004-2014 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kato Y., Hazama A., Yamagami M., Uozumi N.: "Addition of a peptide tag at the C terminus of AtHKTl inhibits its Na^+ transport"Biosci.Biotech.Biochem.. 67. 2291-2293 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Umigai, N., et al.: "Topogenesis of two transmembrane type K^+ channels, Kir 2.1 and KcsA"J.Biol.Chem.. 278. 40373-40384 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zhang, L., Lee, J-K., John, S.A., Uozumi, K., Kadama, I.: "Mechanosensitivity of GIRK channels is mediated by PKC-dependent channel-PIP_2 interaction"J.Biol.Chem.. In press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 水谷昭文, 佐藤陽子, 松田信行, 加藤靖浩, 魚住信之: "植物カリウムイオン輸送系と生理的役割"植物細胞工学別冊 秀潤社. 18. 40-47 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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