| Project/Area Number |
15380071
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
FUKUZAWA Hideya KYOTO UNIVERSITY, Graduate School of Biostudies, Associate Professor, 生命科学研究科, 助教授 (30183924)
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| Co-Investigator(Kenkyū-buntansha) |
大和 勝幸 京都大学, 生命科学研究科, 助手 (50293915)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2004: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2003: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Carbon dioxide / Photosynthetic carbon fixation / nitrate transporter / Chloroplast envelope / DNA array / Zinc finger / Gree algae, Chlamydomonas / Signal transduction / クラミドモナス / cDNAアレイ / 無機炭素濃縮機構 / 低CO_2ストレス / シグナル伝達 / トランスポーター |
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| Research Abstract |
Chlamydomonas modulates photosynthetic characteristics to acclimate to CO_2-limiting stress by sensing CO_2 availability and light by inducing a set of genes for carbon-concentrating mechanism. We have analyzed regulation of a gene Cah1 encoding a carbonic anhydrase to elucidate the CO_2-signal transduction pathways. By using Ars-reporter fused with Cah1 promoter, cis-acting elements and DNA-binding proteins involved in CO_2-regulation were elucidated (Kucho et al. Plant Physiol. 2003, Yoshioka et al. Plant Cell, 2004). Genome wide analysis of gene expression of regulatory mutants revealed that CO_2-responsive genes were grouped into several categories (Miura Plant Physiol., 2004). Although functions of a master regulator CCM1 (Fukuzawa et al. PNAS 2001) is not known, several low-CO_2 inducible genes encoding chloroplast envelop proteins and anion transporters were good candidates for inorganic carbon transporters in eukaryotic algae, Chlamydomonas reinhardtii.
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