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Investigation of signal transduction pathways of jasmonic acid in higher plants

Research Project

Project/Area Number 15380080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

YAMANE Hisakazu  The University of Tokyo, Biotechnology Research Center, Professor (80090520)

Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥7,800,000)
Keywordsjasmonic acid / chitin olieosaccharide elicitor / 12-oxophytodiehoic acid reductase / bHLH transcription factor / WRKY-type transcription factor / defense responses / disease resistance / Orvza aativa L. / エリシター / ジテルペン環化酵素遺伝子 / ファイトアレキシン / 転写因子 / ジテルペン環化酵素 / フィトアレキシン / オリザレキシンS
Research Abstract

In this project, in order to have basic information on jasmonic acid (JA) functions and its signal transduction pathways, we have performed extensive studies including promoter analysis of a jasmonic acid (JA)-responsive gene, functional identification of a JA biosynthetic enzyme gene, and detailed characterization of JA/elicitor-responsive transcription factors that play key roles in the regulation of defense responses in rice. The principal conclusions are as follows: (l) We identified a TGACG-containing sequence that is a cis element involved in the JA-responsiveness of OsOPR1 in suspension-cultured rice cells, suggesting that a basic leucine zipper (bZIP) transcription factor is involved in the JA-responsive expression of OsOPR1 transcription. (2) We provided evidence that an OPR family gene in rice chromosome 8, OsOPR7, encodes the enzyme involved in the JA biosynthesis. The expression of OsOPR7mRNA was induced and reached maximum levels within 0.5 h of mechanical wounding and dro … More ught stress, and the endogenous JA level started to increase in accordance with the increase in OsOPR7expression. (3) We indicated that the JA-responsive bHLH transcription factor gene RERJ1 isolated from suspension-cultured rice cells was expressed in response to exogenous JA in leaves, leaf sheaths, and roots of rice in a vegetative stage, and that RERJ1 expression in the leaves was up-regulated by exposure to wounding and drought stress. In our recent studies including microarray analysis using transgenic rice overexpressing sense-RERJ1 mRNA, it has been shown that RERJ1 is involved in regulation of pathogenesis-related protein genes expression. (4) We provided strong evidence for the involvement of OsWRKY53 and OsWRKY71 in regulating the expression of defense-related genes induced by chitin oligosaccharide and fungal cerebroside elicitors in rice. Although OsWRKY53 was suggested to be a transcriptional activator, OsWRKY71 showed features characteristic of a transcriptional repressor, indicating that the activation of defense-related genes by OsWRKY71 was probably indirect. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (54 results)

All 2008 2007 2006 2005 2004 2003

All Journal Article (16 results) (of which Peer Reviewed: 6 results) Presentation (38 results)

  • [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involyed in the biosynthesis of jasmnic acid in rice.Planta,227:517-526(2008)2008

    • Author(s)
      Tani, T., et. al.
    • Journal Title

      Planta 227

      Pages: 517-526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characterization of an elicitor-induced rice WRKY gene,OsWRKY712008

    • Author(s)
      Chujo, T., et. al.
    • Journal Title

      Biosci.Biotechnol.Biochem 72

      Pages: 240-245

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmnic acidin rice.2008

    • Author(s)
      Tani, T., et. al.
    • Journal Title

      Planta 227

      Pages: 517-526

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of an elicitorinduced rice WRKY gene, OsWRKY71.2008

    • Author(s)
      Chuio, T., et. al.
    • Journal Title

      Biosci Biotechnoi. Biochem. 72

      Pages: 240-245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice1769:497-505(2007)2007

    • Author(s)
      Chujo, T., et. al.
    • Journal Title

      Biophys.Biochem.Acta 1769

      Pages: 497-505

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Identification of a jasmonic acid-responsive region in the promoter of the rice 2-oxophytodienoic acid reductase 1 gcne OsOPR12007

    • Author(s)
      Sobajima, H., et. al.
    • Journal Title

      Diosci.Biotechnol.Biochem 71

      Pages: 3110-3115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of the elicitorinduced gene Os WRKY53 in the expression of defense'rolated genes in rice.2007

    • Author(s)
      Biosci. Biotechnol. Biochem.
    • Journal Title

      Biophys. Biochem. Acta 1769

      Pages: 497-505

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification ofajasmonic acid-responsive region in the promoter of the rice 12-oxophytodienoic acid reductase 1 gene OsOPR1.2007

    • Author(s)
      Sobajima, H., et. al.
    • Journal Title

      Biosci. Biotechnol. Biochem. 71

      Pages: 3110-3115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of the Basic Helix-loop-helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants2005

    • Author(s)
      Kiribuchi, K., et. al.
    • Journal Title

      Biosci.Biotechnol.Biochem 69

      Pages: 1042-1044

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of the Basic Helix-loop-helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants.2005

    • Author(s)
      Kiribtichi, K. et at.
    • Journal Title

      Biosci. Biotechnol. Biochem. 69

      Pages: 1042-1044

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of the basic helix-loop-helix transcription factor RERJ1 in wounding and drought stress responses in rice plants2005

    • Author(s)
      Kiribuchi et al.
    • Journal Title

      Bioscience, Biotechnology, ans Biochemistry 69(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] RERJ1,a jasmonic acid-responsive gene from rice,encodes a basic helix-loop-helix protein2004

    • Author(s)
      Kiribuchi, K., et. al.
    • Journal Title

      Biocbem.Biophys.Res.Commun. 325

      Pages: 857-863

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] RERJ1, a jasmonic acid'tesponsive gene from rice, encodes a basic helix-loop-helix protein.2004

    • Author(s)
      Kiribuchi, K., et. al.
    • Journal Title

      Biochern. Biophys. Res. Common 325

      Pages: 857-863

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice2004

    • Author(s)
      Nemoto et al.
    • Journal Title

      FEBS Letters 571

      Pages: 182-186

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins2004

    • Author(s)
      Jikumaru et al.
    • Journal Title

      Bioscience, Biotechnology, ans Biochemistry 68・7

      Pages: 1461-1466

    • Related Report
      2004 Annual Research Report
  • [Journal Article] RERJ1, a jasmonic acid-responsive gene from rice, encodes a basic helix-loop-helix protein2004

    • Author(s)
      Kiribuchi et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 325

      Pages: 857-863

    • Related Report
      2004 Annual Research Report
  • [Presentation] イネにおけるエリシター応答性WRKY型転写因子OsWRKY71の機能解析2006

    • Author(s)
      山根 久和
    • Organizer
      第47回日本植物生理学会年会
    • Place of Presentation
      つくば
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸生合成に関わる12-oxophytodienaic acid reductase(OPR)の機能解析2006

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2006年度大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY遺伝子のクローニング2006

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2006年度大会
    • Place of Presentation
      京都
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional analysis of an elicitor-responsive WRKY factor, transcription factor, OsWRKY71, in rice.2006

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Plant Physiologists
    • Place of Presentation
      Tsukuba
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional analysis of 12-oxophytodienoic acid reductase(OPR) that is involved in biosynthesis of jasomonic acid in rice.2006

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Cloning of elicitor-responsive WRKY genes in rice.2006

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸生合成に関与する12-oxophytodienoic acid reductase 8(OsOPR8)の機能解析2005

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第40回大会
    • Place of Presentation
      東京
    • Year and Date
      2005-10-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY型転写因子OsWRKY53,OsWRKY71の機能解析2005

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第40回大会
    • Place of Presentation
      東京
    • Year and Date
      2005-10-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Rice 12-oxophytodienoic acid acid reductase 8(OsOPR8) is the isozyme of involved in biosynthesis of jasmonic acid.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-10-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional analysis of elicitor-responsive WRKY factors, OsWRKY53 and OsWRKY71, in rice.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-10-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY型転写因子の機能解析2005

    • Author(s)
      山根 久和
    • Organizer
      第46回日本植物生理学会年会
    • Place of Presentation
      新潟
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸生合成に関わる12-oxophytodienoic acid reductase(OPR)の機能解析2005

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2005年度大会
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるファイトアレキシン生合成とその制御機構2005

    • Author(s)
      山根 久和
    • Organizer
      東京大学・生物生産工学研究センターシンポジウム「イネの病審抵抗性研究の現状と課題」
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional identification of dieterpene cyclases in volved in biosynthesis of rice diteenoid phytoalexins and regulatory mechanisms of their gene expression2005

    • Author(s)
      Yamane, H.
    • Organizer
      Pacifichem 2005
    • Place of Presentation
      Honolulu
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Involvement of elicitor responsive transcription factors,OsWRKY53 and OsWltKY71,in defense responses in rice2005

    • Author(s)
      Yamane, H.
    • Organizer
      Pacifichem 2005
    • Place of Presentation
      Honolulu
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional analysis of elicitor-responsive WRKY genes in rice.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society. for Plant Physiologists
    • Place of Presentation
      Niigata
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional analysis of 12-oxophytodienoic acid reductase(OPR) that is involved in jasomonic acid biosynthesis in rice.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Sapporo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Biosynthesis of rice phytoalexins and its regulatory mechanisms.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Biotechnology Research Center, The University of Tokyo, Symposium
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Functional identification of dieterpene cyclases involved in biosynthesis of rice diterpenoid phytoalexins and regulatory mechanisms of their gene expression.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Pacifichem 2005
    • Place of Presentation
      Honolulu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Involvement of elicitor responsive transcription factors, OsWRKY53 and OsWRKY71, in defense responses in rice.2005

    • Author(s)
      Yamane, H.
    • Organizer
      Pacifichem 2005
    • Place of Presentation
      Honolulu
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY遺伝子の単離・解析2004

    • Author(s)
      山根 久和
    • Organizer
      第45回日本植物生理学会年会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネ培養細胞から単離された12-oxophytodienoic acid reductase遺伝子(OsOPR1)のプロモーターにおけるジャスモン酸応答性シスエレメントの同定2004

    • Author(s)
      山根 久和
    • Organizer
      第45回日本植物生理学会年会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY遺伝子の発現解析2004

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸応答性遺伝子RERJ1の標的遺伝子の同定2004

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸生合成に関わる12-oxophytodienoic acid(OPDA)還元酵素遺伝子の単離と機能解析2004

    • Author(s)
      山根 久和
    • Organizer
      日本農芸化学会2004年度大会
    • Place of Presentation
      広島
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRXYの病害抵抗性への関与2004

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第39回大会
    • Place of Presentation
      秋田
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネのジャスモン酸生合成に関わる12-oxophytodienoic acid(OPDA)還元酵素遺伝子の同定2004

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第39回大会
    • Place of Presentation
      秋田
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Isolation and characterization of elicitor-responsive WRKY genes in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society of for Plant Physiologists
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Identification of a jasmonic acid-responsive cis-element in the promoter of 12-oxophytodienoic acid reductase gene isolated from Suspension-cultured rice cells.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Plant Physiologists
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Expression analysis of elicitor-responsive WRKY genes in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Isolation and functional analysis of cis-12-oxophytodienoic acid(OPDA) reductase gene involved in biosynthesis of jasmonic acid in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] An attempt to identify target genes of the jasmonic acid-responsive transcriptional factor RERJ1 in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japan Society for Bioscience, Biotechnology and Agrochemistry
    • Place of Presentation
      Hiroshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Identification of a gene encoding 12-oxophytodienoic acid(OPDA) reductase involved in biosynthesis of jasmonic acid in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Involvement of elicitor-responsive WRKY genes in defense of responses against pathogen infection in rice.2004

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society for Chemical Regulation of Plants
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネ培養細胞から単離されたジャスモン酸応答性12-oxophytodienoie acid reductase遺伝子(OsOPRI)の5'上流域の解析2003

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第38回大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] イネにおけるエリシター応答性WRKY遺伝子のクローニング2003

    • Author(s)
      山根 久和
    • Organizer
      植物化学調節学会第38回大会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Identification of a jasmonic acid-responsive region in the promoter of a 12-oxophytodienoic acid reductase 1 gene OsOPR1, isolated from suspension-cultured rice cells.2003

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society of for Chemical Regulation of Plants
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Presentation] Isolation and characterization of elicitor-induced genes encoding WRKY DNA-binding proteins from rice.2003

    • Author(s)
      Yamane, H.
    • Organizer
      Annual Meeting of the Japanese Society of for Ckhemical Regulation of Plants
    • Place of Presentation
      Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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