Structure-physicochemical property relationships at molecular levels of seed storage proteins
| Project/Area Number |
15380092
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
UTSUMI Shigeru KYOTO UNIVERSITY, Graduate School of Agriculture, Professor, 農学研究科, 教授 (40111976)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Nobuyuki KYOTO UNIVERSITY, Graduate School of Agriculture, Research Associate, 農学研究科, 助手 (90303908)
安達 基泰 京都大学, 農学研究科, 助手 (60293958)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | seed storage proteins / 7S globulin / 11S globulin / physicochemical property / crystallograbhy / 構造・加工特性相関 / 結晶化 / 豆科種子 / 種子タンパク質 / 溶解性 / 乳化性 / X線結晶構造解析 |
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| Research Abstract |
Most of plant seeds except some cereals contain 7S and/or 11S globulin as major storage proteins in analogy with soybean. It is highly probable that some crops contain 7S and/or 11S globulins having useful properties different from those of soybean, since amino acid seguence identities of them with those of soybean proteins are 35-80%.
1.Physicochemical properties of storage proteins of various crops :
We compared physicochemical properties of 7S and/or 11S from pea, broad bean, azuki bean, kidney bean, pumpkin and bumbara bean at the same condition as that used for soybean proteins. We found that kidney bean 7S exhibits high solubility at low ionic strength and bumbara bean 11S has very low thermal stability.
2.Comparison of physicochemical properties of recombinant and native 7S :
Azuki bean and kidney bean 7Ss were expressed in E.coli. Comparison of physicochemical properties of the recombinant and native 7Ss indicated that carbohydrate moiety contributes to solubility at low ionic strength.
3.X-ray crystallography :
Recombinat soybean 11SA2Bla, pea 11S, pumpkin 11S and azuki bean 7S were subjected to crystallization. All of them except pea 11S gave crystals suitable for X-ray crystallography. We succeeded to determine their structures.
4.Structure-physicochemical Property relationship :
All the data we obtained in this study indicate that molecular surface electrostatic potential is a kea factor of solubility at low ionic strength.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Structure-function relationship of soybean proglycinins at subunit level.2005
Author(s)
Prak, K., Nakatani, K., Katsube-Tanaka, T., Adachi, M., Murayama, N., Utsumi, S.
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Journal Title
J.Agric.Food Chem. 53
Pages: 3650-3657
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular analysis and physicochemical properties of electrophoretic variants of wild soybean Glycine sofa storage proteins.2005
Author(s)
Fukuda, T., Maruyama, N., Kanazawa, A., Abe, J., Shimamoto, Y., Hiemori, M., Tsuji, H., Tanisaka, T., Utusmi, S.
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Journal Title
J.Agric.Food Chem. 53
Pages: 3658-3665
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 8S globulin of mungbean [Vigna radiata (L.) Wilczek] : Cloning and characterization of its cDNA isoforms, expression in Escherichia coli, purification, and crystallization of the major recombinant 8S isoform.2004
Author(s)
Bernardo, A.E.N., Garcia, R.N., Adachi, M, Angeles, J.G.C., Kaga, A., Ishimoto, M., Utsumi, S., Tecson-Mendoza, E.M.
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Journal Title
J.Agiric.Food Chem. 52
Pages: 2552-2560
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gelling properties of soybean β-conglycinin having different subunit compositions.2004
Author(s)
Mohamad Ramlan M.S., Maruyama, N., Takahashi, K., Yagisaki, K., Higasa, T., Matsumura, Y., Utsumi, S.
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Journal Title
Biosci.Biotechnol.Bioc 68
Pages: 1091-1096
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Structure-physicochemical function relationships of soybean glycinin at subunit level assessed by using mutant lines.2004
Author(s)
Maruyama, N., Prak, K., Motoyama, S., Choi, S.K., Yagasaki, K., Ishimoto, M., Utsumi, S.
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Journal Title
J.Agric.Food Chem. 52
Pages: 8197-8201
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular and structural analysis of electrophoretic variants of soybean seed storage proteins.2003
Author(s)
Maruyama, N., Fukuda, T., Saka, S., Inui, N., Kotoh, J., Miyagawa, M., Hayashi, M., Sawada, M., Moriyama, T., Utsumi, S.
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Journal Title
Phytochemistry 64
Pages: 701-708
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Crystal structure of soybean 11S globulin : Glycinin A3B4 homohexamer.2003
Author(s)
Adachi, M., Kanamori, J., Masuda, T., Yagisaki, K., Kitamura, K., Mikami, B., Utsumi, S.
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Journal Title
Proc.Natl.Acad.Sci.USA 100
Pages: 7395-7400
Description
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[Journal Article] Crystal structures and structural stabilities of the disulfide-deficient soybean proglycinin mutants C12G and C88S.2003
Author(s)
Adachi, M., Okuda, E., Kaneda, Y., Hadhimoto, A., Shutov, A.D., Becker, C., Muntz, K., Utsumi, S.
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Journal Title
J.Agric.Food Chem. 51
Pages: 4633-4639
Description
「研究成果報告書概要(欧文)」より
Related Report
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