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Stock identification of cultured fish and shellfish by composition of trace elements in the hard tissues : a new technology for identification of product origin.

Research Project

Project/Area Number 15380125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

TAKAGI Yasuaki  Hokkaido Univ., Faculty of Fish.Sci., Professor, 大学院・水産科学研究院, 教授 (10212002)

Co-Investigator(Kenkyū-buntansha) ARAI Takaomi  Univ.of Tokyo, Ocean Res.Inst., Research Associate, 海洋研究所, 助手 (70323631)
ABE Syuiti  Hokkaido Univ., Faculty of Fish.Sci., Professor, 大学院・水産科学研究院, 教授 (80125278)
YAMAHA Etsuro  Hokkaido Univ., Field Science Center for Northern Biosphere, Professor, 北方生物圏フィールド科学センター, 教授 (60191376)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsOtolith / Trace-elemental fingerprints / Stock identification / Product origin identification / Teleost fishes / Shellfishes / Migration patterns / サクラマス / ホタテガイ
Research Abstract

For trace-elemental fingerprint studies in fish otoliths, industry-coupled plasma mass spectrography (ICPMS) has not used in Japan. The present study was undertaken to develop ICPMS-based trace-elemental fingerprint studies in the hard tissues of cultured fish and shellfish in Japan. Following results were obtained.
1. We developed standard methods to determine trace-elemental composition of fish otoliths and shells of shellfish using solution-based and laser abration ICPMS. Trace-elemental compositions were useful fingerprints that could identify product origin of cultured species.
2. We clarified migratory patterns of some salmonid and eel species using Sr contents and Sr/Ca ratio of otoliths determined by EPMA and revealed that the method is a powerful tool to study migration patterns of anadromous, catadromous or diadromous fishes.
3. We developed a DNA microarray to identify stocks of chum salmon. The array was accurate and time effective, and required no special equipments such as DNA sequencer. Therefore, the method is especially a powerful tool to identify chum salmon stocks in the field study and on board.
4. We immunohistochemically localized otolith matrix proteins, OMP-1 and otolin-1, and suggested important rolls of these proteins in calcification and formation of otolith micro structures. We also revealed that the aragonite saturation ratio of endolymph was maintained over 1.0 through the day-night cycle, suggesting aragonite crystal growth could be continued through a day. On the other hand, mRNA expression of otolin-1 showed clear daily rhythm with significantly higher expressions during night-time, suggesting a key fuction of otolin-1 to construct daily rings. In masu salmon, increases in Sr concentrations and Sr/Ca ratios in the serum occurred 7 days after the seawater transfer. Such a time-lag must be taken into account to reconstruct migration patterns in the anadromous fishes.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (20 results)

All 2005 2004 2003

All Journal Article (19 results) Book (1 results)

  • [Journal Article] Diel changes in endolymph aragonite saturation rate and mRNA expression of otolith matrix proteins in the trout otolith organ.2005

    • Author(s)
      Takagi, Y et al.
    • Journal Title

      Marine Ecology Progress Series 294

      Pages: 249-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 魚類における耳石形成2005

    • Author(s)
      都木靖彰
    • Journal Title

      海洋生命系のダイナミクス-2, 海洋生物の機能(東京大学海洋研究所)(竹井祥郎 編)(東海大学出版会)

      Pages: 312-327

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seasonal variation in the migratory history of the Japanese eel Anguilla japonica in Mikawa Bay, Japan2005

    • Author(s)
      Kotake, A et al.
    • Journal Title

      Marine Ecology Progress Series 293

      Pages: 213-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Small anadromous Salvelinus malma at the southern limits of its distribution.2005

    • Author(s)
      Morita, K
    • Journal Title

      Journal of Fish Biology 66

      Pages: 1187-1192

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diel changes in endolymph aragonite saturation rate and mRNA expression of otolith matrix proteins in the trout otolith organ.2005

    • Author(s)
      Takagi Y, et al.
    • Journal Title

      Marine Ecology Progress Series 294

      Pages: 249-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seasonal variation in the migratory history of the Japanese eel, Anguilla japonica, in Mikawa Bay, Japan.2005

    • Author(s)
      Kotake A, et al.
    • Journal Title

      Marine Ecology Progress Series 293

      Pages: 213-221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Small anadromous dolly varden on the southern limit of its distribution, as revealed by otolith Sr:Ca ratios.2005

    • Author(s)
      Morita K, et al.
    • Journal Title

      Journal of Fish Biology 66

      Pages: 1187-1192

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Diel changes in endolymph aragonite saturation rate and mRNA expression of otolith matrix proteins in the trout otolith organ.2005

    • Author(s)
      Takagi, Y., H.Tohse, E.Murayema, T.Ohira, H.Nagasawa
    • Journal Title

      Marine Ecology Progress Series 294

      Pages: 249-256

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 魚類における耳石形成2005

    • Author(s)
      都木靖彰
    • Journal Title

      東京大学海洋研究所/海洋生命系のダイナミクス-2,海洋生物の機能(竹井祥郎編)(東海大学出版会)

      Pages: 312-327

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Seasonal variation in the migratory history of the Japanese eel Anguilla japonica in Mikawa Bay, Japan2005

    • Author(s)
      Kotake, A, Okamura, A, Yamada, Y, Utoh, T, Arai, T, Miller, MJ, Oka, HP, Tsukamoto, K
    • Journal Title

      Marine Ecology Progress Series 293

      Pages: 213-221

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Small anadromous Salvelinus malma at the southern limits of its distribution.2005

    • Author(s)
      Morita, K, Arai, T, Kishi, D, Tsuboi, J
    • Journal Title

      Journal of Fish Biology 66

      Pages: 1187-1192

    • Related Report
      2005 Annual Research Report
  • [Journal Article] DNA microarray for rapid detection of mitochondrial DNA haplotypes of chum salmon.2004

    • Author(s)
      Moriya S et al.
    • Journal Title

      Marine Biotechnology 6

      Pages: 430-434

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic population structure of chum salmon in the Pacific Rim inferred from mitochondrial DNA sequence variation.2004

    • Author(s)
      Sato S et al.
    • Journal Title

      Environmental Biology of Fishes 69

      Pages: 38-50

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] DNA microarray for rapid detection of mitochondrial DNA haplotypes of chum salmon.2004

    • Author(s)
      Moriya S, et al.
    • Journal Title

      Marine Biotechnology 6

      Pages: 430-434

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic population structure of chum salmon in the Pacific Rim inferred from mitochondrial DNA sequence variation.2004

    • Author(s)
      Sato S, et al.
    • Journal Title

      Environmental Biology of Fishes 69

      Pages: 38-50

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occurrence of the non-anadromous life history in the shirauo, Salangichthys microdon2004

    • Author(s)
      Arai, T.et al.
    • Journal Title

      Journal of Applied Ichthyology 20

      Pages: 238-240

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Evidence of downstream migration of Sakhalin taimen, Hucho perryi, as revealed by Sr:Ca ratios of otolith.2004

    • Author(s)
      Arai, T.et al.
    • Journal Title

      Ichthyological Research 51

      Pages: 377-380

    • NAID

      10014107079

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Alkaline earth metal and Mn distribution in otoliths of Anguilla spp.glass eels and elvers.2003

    • Author(s)
      Arai, T.et al.
    • Journal Title

      Fisheries Science 69

      Pages: 421-423

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Migratory histories of three types of Cottus pollux (small-egg, middle-egg, and large-egg types), as revealed by otolith microchemistry.2003

    • Author(s)
      Goto, A.et al.
    • Journal Title

      Ichthyological Research 50

      Pages: 67-72

    • Related Report
      2004 Annual Research Report
  • [Book] Otolith formation in teleost fishes. (in Japanese) In : Dynamics of Marine Life Systems - 2. Physiological Functions of Marine Organisms, (Takei Y, ed)2005

    • Author(s)
      Takagi Y
    • Publisher
      Tokai University Press
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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