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Studies about Bacteria Quantification using Molecular Biological Method and Mechanism of Biogas Production.

Research Project

Project/Area Number 15380188
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionNational Agricultural and Bio-oriented Research Organization

Principal Investigator

TAKENAKA Akio  National Institute of Livestock and Grassland Science, Department of Physiology and Nutrition, Head of Laboratory, 畜産草地研究所・家畜生理栄養部, 室長 (40155031)

Co-Investigator(Kenkyū-buntansha) KAJIKAWA Hiroshi  National Institute of Livestock and Grassland Science, Department of Physiology and Nutrition, Senior Researcher, 畜産草地研究所・家畜生理栄養部, 主任研究官
MITSUMORI Makoto  National Institute of Livestock and Grassland Science, Department of Physiology and Nutrition, Senior Researcher, 畜産草地研究所・家畜生理栄養部, 主任研究官 (40418588)
TAJIMA Kiyoshi  National Institute of Livestock and Grassland Science, Department of Physiology and Nutrition, Senior Researcher, 畜産草地研究所・家畜生理栄養部, 主任研究官 (80343953)
OSADA Takashi  National Research Center for Hokkaido Region, Department of Livestock and Grassland Science, Team Leader, 畜産草地部, チーム長 (90355090)
栗原 光規  独立行政法人農業・生物系特定産業技術研究機構, 畜産草地研究所・家畜生理栄養部, 室長 (30355060)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2005: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2003: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsMethane / PCR / Semiconductor Gas Sensor / Green House Gas / Rumen Microorganism / 半導体センサー / 温暖化ガス / 水素 / ルーメン / メタン菌 / 定量的PCR
Research Abstract

The concentrations of methane and hydrogen gas were measured using a semiconductor gas monitor system (mBA-3000) provided for medical use. There was no correlation between the methane and hydrogen concentrations, or between methane and nitrous oxide concentrations. There was a week positive correlation between the methane and ammonia concentrations. We invented a methane and hydrogen gas monitor (mBA-5000) for the expired breath of ruminant animals using semiconductor gas sensor. This monitor can detect the concentration of methane (1 to 1000 ppm) and hydrogen (1 to 100 ppm) every 10 seconds for 20 hours, and the stored data can convert to a personal computer via serial port. The mBA-5000 was placed in the metabolic chamber in which a Holstein cow was feeding. The concentrations of methane gas were increased after feeding and peaked at around 1 hour after feeding, and were keeping 600 to 1000 ppm all day long. The hydrogen gas production was observed from 5 to 15 minutes after feeding, and peaked at 30 minutes after feeding. The hydrogen production returned to 0 ppm until 150 minutes after feeding, and had been keeping 0 ppm until the next feeding. This observation reads to a speculation that the hydrogen gas production after feeding might be the overflow of hydrogen or proton produced by the rumen fermentation. The capacity of methane producing rate should be different between the animals, and actually the peak levels of hydrogen gas were varied from 20 to 90 ppm depending on the cattle used in this experiment. PCR detection and quantification of methanogen was provided using the primers constructed from the sequences of 16S ribosomal RNA gene and methyl coenzyme M reductase gene. The results of PCR analysis indicated that the methanogens of the faunated rumen differed significantly from those of the unfaunated rumen.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (54 results)

All 2007 2006 2005 2004 2003 Other

All Journal Article (41 results) Book (8 results) Publications (5 results)

  • [Journal Article] Evaluation procedure of greenhouse gas emission from livestock waste management systems in Japan.2007

    • Author(s)
      Takashi Osada
    • Journal Title

      Global perspective on greenhouse gas emission from agricultural sector, Edited by The organizing committee of OASERD-APEID (Elsevier Science) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Real-Time PCR Detection of the Effects of Protozoa on Rumen Bacteria in Cattle.2006

    • Author(s)
      Yuhei Ozutsumi
    • Journal Title

      Current Microbiology 52・2

      Pages: 158-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Influence of temperature and pH on fermentation patterm and methane production in the rumen simulating fermenter (RUSITEC).2006

    • Author(s)
      Raghavendra Bhatta
    • Journal Title

      Asian-Austlarian Journal of Animal Science 19・3

      Pages: 376-380

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Real-Time PCR Detection of the Effects of Protozoa on Rumen Bacteria in Cattle.2006

    • Author(s)
      Yuhei Ozutsumi, Kiyoshi Tajima, Akio Takenaka, Hisao Itabashi
    • Journal Title

      Current Microbiology 52

      Pages: 158-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Influence of temperature and pH on fermentation patterm and methane production in the rumen simulating fermenter (RUSITEC).2006

    • Author(s)
      Raghavendra Bhatta, Kiyoshi, Tajima, Mitsunori Kurihara
    • Journal Title

      Asian-Austlarian Journal of Animal Science 19(3)

      Pages: 376-380

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Control of Rumen Microbial Fermentation for Mitigating Methane Emissions from the Rumen.2006

    • Author(s)
      Makoto Mitsumori, Weibin Sun
    • Journal Title

      Proceedings for "2006 International Symposium on Recent Advance in Animal Nutrition"

      Pages: 35-45

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Measurement of Hydrogen Gas in the Expiration of Cattle by a Semiconductor Gas Monitor.2006

    • Author(s)
      Akio Takenaka, Kiyoshi Tajima, Makoto Mitsumori, Hiroshi Kajikawa, Hideo Ueda
    • Journal Title

      XIIth AAAP Animal Science Congress 2006 Congress Proceedings

      Pages: 88-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Effect of Protozoa on Rumen Bacteria and Methanogens of Cattle Using 16S rDNA and MCRA Gene.2006

    • Author(s)
      Yuhei Ozutsumi, Kiyoshi Tajima, Akio Takenaka, Hisao Itabashi
    • Journal Title

      XIIth AAAP Animal Science Congress 2006 Congress Proceedings

      Pages: 574-575

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Better evaluation system for N20 and CH4 emission from composting (and wastewater purification) of Livestock waste.2006

    • Author(s)
      Takashi Osada
    • Journal Title

      Greenhouse Gas Inventory Development in Asia : Experiences from Workshops on Greenhouse Gas Inventories in Asia. ISSN 1341-4356, CGER-I067-2006, (National Institute for Environmental Studies)

      Pages: 78-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Influence of temperature and pH on fermentation patterm and methane production in the rumen simulating fermenter (RUSITEC).2006

    • Author(s)
      Raghavendra Bhatta, Kiyoshi Tajima, Mitsumori Kurihara
    • Journal Title

      Asian-Austlarian Journal of Animal Science 19・3

      Pages: 376-380

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Real-Time PCR Detection of the Effects of Protozoa on Rumen Bacteria in Cattle.2006

    • Author(s)
      Yuhei Ozutsumi, Kiyoshi Tajima, Akio Takenaka, Hisao Itabashi
    • Journal Title

      Current Microbiology 52・2

      Pages: 158-162

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The effect of protozoa on the composition of rumen bacteria in cattle by using 16S rRNA gene clone libraries.2005

    • Author(s)
      Yuhei Ozutsumi
    • Journal Title

      Bioscience Biotechnology and Biochemistry 69

      Pages: 499-506

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ルーメンプロトゾアの繊維分解酵素とその遺伝子解析.2005

    • Author(s)
      竹中 昭雄
    • Journal Title

      栄養生理研究会報 49・1

      Pages: 65-79

    • NAID

      10015561951

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Animo acids antagonistic to the amino acids inhibitory for growth rate of mixed ruminal bacteria.2005

    • Author(s)
      Hiroshi Kajikawa
    • Journal Title

      Journal of Dairy Science 88

      Pages: 2601-2603

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 環境への影響をどのように評価するか2005

    • Author(s)
      長田 隆
    • Journal Title

      北海道家畜管理研究会報 40

      Pages: 6-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effect of protozoa on the composition of rumen bacteria in cattle by using 16S rRNA gene clone libraries.2005

    • Author(s)
      Yuhei Ozutsumi, Kiyoshi Tajima, Akio Takenaka, Hisao Itabashi
    • Journal Title

      Bioscience Biotechnology and Biochemistry. 69

      Pages: 499-506

    • NAID

      130000030387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fiber degrading enzymes and gene analysis from the rumen ciliate protozoa.2005

    • Author(s)
      Akio Takenaka, Hisao Itabashi
    • Journal Title

      Proceedings of Japanese Society for Animal Nutrition and Metabolism 49(1)

      Pages: 65-79

    • NAID

      10015561951

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Animo acids antagonistic to the amino acids inhibitory for growth rate of mixed ruminal bacteria.2005

    • Author(s)
      Hiroshi Kajikawa, Makoto Mitumori, Kiyoshi Tajima, Mitsunori Kurihara
    • Journal Title

      Journal of Dairy Science 88

      Pages: 2601-2603

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of methanogens from the rumen by PCR-single-strand conformation polymorphism (PCR-SSCP).2005

    • Author(s)
      Makoto Mitsumori, Noriko Tatsuoka, Mitsunori Kurihara, Kiyoshi Tajima, Hiroshi Kajikawa, Hisao Itabashi
    • Journal Title

      Proceeding of Greenhouse Gas and Animal Agriculture 2005

      Pages: 422-423

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of methane and nitrous oxide emission from compostine of pig. manure.2005

    • Author(s)
      Takashi OSADA, Yasuyuki FUKUMOTO, Makoto SHIRAISHI, Keiichi UCHIDA, Nobuyuki WAKIMOTO
    • Journal Title

      Proceeding of Greenhouse Gas and Animal Agriculture 2005

      Pages: 509-512

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Animo acids antagonistic to the amino acids inhibitory for growth rate of mixed ruminal bacteria.2005

    • Author(s)
      Hiroshi Kajikawa, Makoto Mitumori, Kiyoshi Tajima, Mitsunori Kurihara
    • Journal Title

      Journal of Dairy Science 88・7

      Pages: 2601-2603

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ルーメンプロトゾアの繊維分解酵素とその遺伝子解析2005

    • Author(s)
      竹中昭雄, 板橋久雄
    • Journal Title

      栄養生理研究会報 49・1

      Pages: 65-79

    • NAID

      10015561951

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 配列特異的SSU rRNA切断法を用いたウシルーメン細菌叢の定量的解析.2004

    • Author(s)
      上野 豊
    • Journal Title

      ルーメン研究会報 15・2

      Pages: 55-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fiber Digestion by Rumen Ciliate Protozoa.2004

    • Author(s)
      Akio Takenaka
    • Journal Title

      Microbes & Environment 19

      Pages: 203-210

    • NAID

      110001269377

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 畜舎内のアンモニア、メタン及び亜酸化窒素の濃度と舎内環境.2004

    • Author(s)
      長田 隆
    • Journal Title

      におい・かおり環境学会誌(旧においの研究) 35

      Pages: 21-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 亜酸化窒素・メタンの発生抑制方法の検討-肥育牛ふんの堆肥化過程から発生するアンモニア・亜酸化窒素・メタン濃度-.2004

    • Author(s)
      白石 誠
    • Journal Title

      岡山総総合畜産センター研究報告 15

      Pages: 70-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantitative analysis of ruminal bacterial populations of dairy cattle by sequence-specific rRNA cleavage method.2004

    • Author(s)
      Y.Ueno, Y.Sekiguchi, K.Tajima, A.Takenaka, Y.Kamagata
    • Journal Title

      Proceedings of Japanese Society for Rumen Metabolism and Physiology 15(2)

      Pages: 55-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fiber Digestion by Rumen Ciliate Protozoa.2004

    • Author(s)
      Akio Takenaka, Kiyoshi Tajima, Makoto Mitsumori, Hiroshi Kajikawa
    • Journal Title

      Microbes and Environment. 19

      Pages: 203-210

    • NAID

      110001269377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Method of controlling generation of nitrous oxide and methane.2004

    • Author(s)
      M.Shiraishi, T.Osada, E.Takimoto, N.Wakimoto, N.Kitamura, K.Okuda
    • Journal Title

      Okayama-ken Sogo Chikusan Center Kenkyu Houkoku 15

      Pages: 70-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Measurement of hydrogen and methane gas using semiconductor chip sensor, and its application for animal production and environmental analysis.2004

    • Author(s)
      Akio Takenaka, Kiyoshi Tajima, Makoto Mitsumori, Hiroshi Kajikawa, Mitsunori Kurihara, Takashi Osada
    • Journal Title

      Proceedings of the 11th AAAP Congress 3

      Pages: 432-434

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fiber Digestion by Rumen Ciliate Protozoa2004

    • Author(s)
      Akio Takenaka, Kiyoshi Tajima, Makoto Mitsumori, Hiroshi Kajikawa
    • Journal Title

      Microbes and Environment 19・3

      Pages: 203-210

    • NAID

      110001269377

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 配列特異的SSUrRNA切断法を用いたウシルーメン細菌叢の定量的解析2004

    • Author(s)
      上野豊, 関口勇地, 田島清, 竹中昭雄, 鎌形洋一
    • Journal Title

      ルーメン研究会報 15・2

      Pages: 55-58

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Methane production of Ongole crossbreed and Limousin crossbreed young bulls under intensive feeding management in Indonesia2004

    • Author(s)
      Agung Purnomoadi, M.Y.Effendi, Edi Rainto, Kohji Higuchi, Mitsunori Kurihara
    • Journal Title

      Proceedings of the 11th AAAP Congress 2004 2

      Pages: 216-218

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Measurement of hydrogen and methane gas using semiconductor chip sensor, and its application for animal production and environmental analysis2004

    • Author(s)
      Akio Takenaka, Kiyoshi Tajima, Makoto Mitsumori, Hiroshi Kajikawa, Mitsunori Kurihara, Takashi Osada
    • Journal Title

      Proceedings of the 11th AAAP Congress 2004 3

      Pages: 432-434

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Methane and Nitrous Oxide Emission from Composting of Pig Manure2004

    • Author(s)
      Takashi OSADA, Yasuyuki FUKUMOTO, Makoto SHIRAISHI, Keiichi UCHIDA, Nobuyuki WAKIMOTO, Tadashi TAMURA, Makoto ISHIBASHI
    • Journal Title

      2004 CIGR International Conference

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 家畜ふんの堆積型堆肥化過程からの環境負荷ガス発生2004

    • Author(s)
      長田 隆, 田村 忠, 白石 誠, 石橋 誠, 福本 泰之
    • Journal Title

      第15回廃棄物学会研究発表会論文集

      Pages: 527-529

    • NAID

      130007005945

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Methane oxidation and its coupled electron-sink reactions in ruminal fluid.2003

    • Author(s)
      Hiroshi Kajikawa
    • Journal Title

      Letters in Applied Microbiology 36

      Pages: 354-357

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PCRを用いたルーメン細菌の特異的な検出2003

    • Author(s)
      田島 清
    • Journal Title

      ルーメン研究会報 14・2

      Pages: 69-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 岡山県内の酪農場から発生する環境負荷ガス実態調査.2003

    • Author(s)
      白石 誠
    • Journal Title

      岡山総総合畜産センター研究報告 14

      Pages: 71-76

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Newbold, Methane oxidation and its coupled electron-sink reactions in ruminal fluid.2003

    • Author(s)
      Hiroshi Kajikawa, C.Valdes, K.Hillman, R.J.Wallace, C.J.
    • Journal Title

      Letters in Applied Microbiology 36

      Pages: 354-357

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PCR detection of rumen bacteria based on 16S rDNA sequence.2003

    • Author(s)
      K.Tajima, M.Mitsumori, H.Kajikawa, A.Takenaka
    • Journal Title

      Proceedings of Japanese Society for Rumen Metabolism and Physiology 14(2)

      Pages: 69-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 呼気生化学の進歩《8》2006

    • Author(s)
      竹中 昭雄
    • Publisher
      日本呼気病態生化学研究会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Non-CO2 Greenhouse Gases (NCGG-4), coordinated by A. van Amstel,20052005

    • Author(s)
      Takashi OSADA
    • Publisher
      Millpress, Rotterdam, ISBN 90 5966 043 9
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 続・環境負荷を予測する(波多野隆介, 犬伏和之編)2005

    • Author(s)
      長田 隆
    • Publisher
      博友社、ISBN4-8268-0202-1
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Non-CO2 Greenhouse Gases (NCGG-4)(coordinated by A.van Amstel) 20052005

    • Author(s)
      Takashi OSADA, Yasuyuki FUKUMOTO, Tadashi TAMURA, Makoto SHIRAISHI, Makoto ISHIBASHI
    • Publisher
      Millpress, Rotterdam, ISBN 90 5966 043 9
    • Related Report
      2005 Annual Research Report
  • [Book] 続・環境負荷を予測する(波多野隆介・犬伏和之編)(博友社)ISBN4-8268-0202-12005

    • Author(s)
      長田 隆
    • Publisher
      博友社、ISBN4-8268-0202-1
    • Related Report
      2005 Annual Research Report
  • [Book] 新ルーメンの世界2004

    • Author(s)
      竹中 昭雄
    • Publisher
      社団法人 農山漁村文化協会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 難培養微生物研究の最新技術-未利用微生物資源へのアプローチ-2004

    • Author(s)
      竹中 昭雄
    • Publisher
      株式会社 シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 難培養微生物研究の最新技術 未利用微生物資源へのアプローチ2004

    • Author(s)
      竹中 昭雄 他
    • Total Pages
      265
    • Publisher
      シーエムシー出版
    • Related Report
      2004 Annual Research Report
  • [Publications] 竹中昭雄, 田島清, 三森真琴, 梶川博: "ルーメンプロトゾア由来の繊維分解酵素遺伝子のコドン使用率"ルーメン研究会報. 14・2. 65-68 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田島清, 三森真琴, 梶川博, 竹中昭雄: "PCRを用いたルーメン細菌の特異的な検出"ルーメン研究会報. 14・2. 69-70 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mutsumi Nakamura, Takafumi Nagamine, Chisato Harada, Kiyoshi Tajima, Hiroki Matsui, Yoshimi Bennno: "Expression of Ruminococcus albus xylanase gene (xynA) in Streptococcus bovis 12-U-1."Current Microbiology. 47・1. 71-74 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroshi Kajikawa, C.Valdes, K.Hillman, R.J.Wallance, C.J.Newbold: "Methane oxidation and its coupled electron-sink reactions in ruminal fluid"Letters in Applied Microbiology. 36. 354-357 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 竹中 昭雄: "新ルーメンの世界"社団法人 農山漁村文化協会. (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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