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Mechanism of Prion Protein Absorption from Digestive Tract in Calves

Research Project

Project/Area Number 15380189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionTohoku University

Principal Investigator

OBARA Yoshiaki  TOHOKU UNIVERSITY, Graduate School of Agricultural Science, professor, 大学院・農学研究科, 教授 (50302196)

Co-Investigator(Kenkyū-buntansha) KATOH Kazuo  TOHOKU UNIVERSITY, Graduate School of Agricultural Science, Associate Professor, 大学院・農学研究科, 助教授 (60091831)
HAGINO Akihiko  TOHOKU UNIVERSITY, Graduate School of Agricultural Science, Assistant Professor, 大学院・農学研究科, 助手 (80156249)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 2004: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2003: ¥11,000,000 (Direct Cost: ¥11,000,000)
KeywordsBovine Spongiform Encephalophasy (BSE) / Abnormal prion protein / Horseradish peroxidase (HRP) / Development of digestive tract / Small intestine / M cell / the method with eventual sacks / Ussing chamber method / Hourseradish Peroxidase (HRP) / ホルスタイン子ウシ / ウサギ / 空腸と回腸 / 消化管の発達 / HRP吸収機構 / 牛海綿状脳症<BSE> / Horseradish Peroxidase / ホルスタイン雄仔ウシ / 回腸 / 離乳
Research Abstract

Although it has been suggested that abnormal Prion proteins are absorbed from the intestinal mucosa and infect many animal species with bovine spongiform encephalopathy (BSE), the detailed mechanisms remain clarified. The aim of the present study was to investigate Horseradish peroxidase (HRP) absorption, in which HRP was used instead of abnormal Prion proteins, and its mechanism in isolated segments of the duodenum, jejunum, ileum and colon from rabbits, sheep and Holstein calves by the methods with everted sacks and the Ussing chamber. We also observed morphological features of M cells in Peyer's patches by the immunochemical method and SEM.
1)In calves, the HRP absorption was greater in the duodenum, jejunum and ileum than in the colon, and also was greater at the age of 13 weeks than at 4 weeks.
2)In rabbits, the HRP absorption showed a tendency to be greater in the ileum than in the colon, and was dependent on aging, the intestine, H^+ gradient and Na^+/K^+-ATPase activities.
3)In calves, the HRP absorption in the jejunum and ileum was greater at the age of 6 weeks than of 15 weeks, and was significantly decreased by the reduction of the medium pH from 7.4 to 5.4. It was also significantly increased by an addition of amiloride but not by ouabain.
4)In the calf jejunal epithelium, it was indicated that M cells increase the cell number and promote the differentiation as the ruminal function develops, and accelerate the ability for absorption of higher molecules.
These findings strongly suggest the possibility that the absorption of abnormal Prion proteins occur at the small intestine, mainly the ileum, after weaning.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2005 2004

All Journal Article (8 results)

  • [Journal Article] Expression of mRNA for SGLT1 and CD36 in the ruminant gastrointestinal tract before and after werning2005

    • Author(s)
      Hayashi H., T.Yonezazwa, T.Kanetani, F.Terada, K.Katoh, Y.Obara
    • Journal Title

      Animal Science Journal 76巻4号(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of mRNA for Na+/glucose transporter 1 (SGLT1) and fatty acid translocase (CD36) in the ruminant gastrointestinal tract before and after weaning.2005

    • Author(s)
      Hayashi E. et al.
    • Journal Title

      Animal Science Journal Vol.76,No2(in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of mRNA for SGLT1 and CD36 in the ruminant gastrointestinal tract before and after werning2005

    • Author(s)
      Hayashi H., T.Yonezazwa, T.Kanetani, F.Terada.K.Katoh, Y.Obara
    • Journal Title

      Animal Science Journal 76巻4号(掲載決定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of mRNA for nutrient transporter in the gastrointesitinal tract before and after werning2004

    • Author(s)
      Hayashi H., T.Yonezazwa, T.Kanetani, K.Katoh, Y.Obara
    • Journal Title

      Journal of Animal and Feed Science 13巻 Suppl.1

      Pages: 405-408

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 乳牛分野における栄養生理研究の近年の進歩2004

    • Author(s)
      小原嘉昭, 寺田文典
    • Journal Title

      栄養生理研究会報 48巻、2号

      Pages: 59-77

    • NAID

      10013755197

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Expression of mRNA for nutrient transporters in the gastrointestinal Tract before and after weaning2004

    • Author(s)
      Hayashi E. et al.
    • Journal Title

      Journal of Animal and Feed Science Vol.13 Suppl1

      Pages: 405-408

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Modern progress in nutirition and metabolism in dairy cows2004

    • Author(s)
      Obara Y., F.Terada
    • Journal Title

      Proceedigs of Japanese society for Animal Nutrition and Metabolism Vol.48

      Pages: 59-77

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Expression of mRNA for nutrient transporter in the gastrointesitinal tract before and after werning2004

    • Author(s)
      Hayashi H., T.Yonezazwa, T.Kanetani.K.Katoh, Y.Obara
    • Journal Title

      Journal of Animal and Feed Science 13, suppl.1

      Pages: 405-408

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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