| Project/Area Number |
15380194
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Nagoya University |
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Principal Investigator |
KITA Kazumi Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (20221913)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kunioki Kagoshima University, Faculty of Agriculture, Professor, 農学部, 教授 (80041656)
TSUKADA Hikaru Nagoya University, Graduate School of Bioagricultural Sciences, Assisitant Professor, 大学院・生命農学研究科, 助手 (20343212)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2003: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | chicken embryo myoblast / muscle / Medium 199 / Protein synthesis= / protein degradation / leucine / methionine / glycine / ニワトリ / 飼料蛋白質 / 3メチルヒスチジン |
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| Research Abstract |
Influence of methionine and/or glycine deficiency on protein synthesis of chicken embryo myoblasts cultured in Medium 199 was examined. Protein synthesis cultured in Medium 199 in which all ingredients were solved in Dulbecco's modified phosphate buffered saline was dramatically lower than that in Medium 199 in which all ingredients were solved in water. Although methionine deficiency from Medium 199 decreased myoblast protein synthesis, glycine deficiency did not affect. There was no interaction between methionine and glycine deficiency on myoblast protein synthesis.
The influence of leucine and fetal calf serum (FCS) in Medium 199 on protein synthesis and degradation of chicken embryo myoblasts was examined. Medium 199 included 0, 1, 2, 5, 10% FCS and 457 (original concentration of leucine in Medium 199), 2287, 4574μmol leucine/l. There was an interaction between leucine and FCS concentrations on muscle protein synthesis in vitro. When FCS was not included in Medium 199, myoblast prot
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ein synthesis was stimulated with an increment in leucine concentration in the medium from 2287 to 4574 μmol/l. When 1 or 2% of FCS was included in Medium 199, myoblast protein synthesis was stimulated by increasing leucine concentration in the medium from 457 to 2287 μmol/l, and at the higher level of leucine, protein synthesis was not stimulated. At more than 5% of FCS in the medium, myoblast protein synthesis at higher concentration of luecine of 4574μmol/l was significantly lower than those of lower leucine levels.
No interaction between leucine and FCS concentrations on muscle protein degradation Was observed. Protein degradation of chicken embryo myoblasts was decreased by an increment of FCS concentration in the medium from 0 to 5%, and at the higher level no significant influence was observed. When leucine concentration of in the medium was increased from 457 to 2287μmol/l, protein degradation of chicken embryo myoblasts was decreased. But high concentration of leucine at 4457μmol/l did not affect protein degradation of chicken embryo myoblasts. Less
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