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Development of combination vaccine by using cytokine expressing transgenic plants.

Research Project

Project/Area Number 15380198
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Principal Investigator

MATSUMURA Takeshi  National Institute of Advanced Industrial Science and Technology, Institute for Biological Resources and Functions Plant Molecular Technology Research Group, Leader, ゲノムファクトリー研究部門・植物分子工学研究グループ, グループリーダー (50358040)

Co-Investigator(Kenkyū-buntansha) MATSUO Kouki  National Institute of Advanced Industrial Science and Technology, Institute for Biological Resources and Functions Plant Molecular Technology Research Group, ゲノムファクトリー研究部門・植物分子工学研究グループ, 研究員 (10358012)
SUGIMOTO Chihiro  National Institute of Advanced Industrial Science and Technology, National Research Center for Protozoan Diseases Research Unit for Advanced Preventive Medicine, 原虫病研究センター, 教授 (90231373)
TSUNEMITSU Hiroshi  National Institute of Animal Health, Shichinohe Research Unit Pasture Disease Section, 動物衛生研究所, 研究室長 (10355159)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2003: ¥8,200,000 (Direct Cost: ¥8,200,000)
Keywordscytokine / rotavirus / transgenic plants
Research Abstract

Rotavirus is one of the most significant pathogens causing diarrhea in mammals. We have already produced the transgenic potato plants expressing rotavirus VP6 cDNA gene which encodes a coat protein. In addition, we have confirmed the antigenicity of the transgenic potato in mice.
In this study we produced the transgenic strawberries which were introduced human interferon(hIFN) gene. Also we have produced the transgenic strawberry expressing both bovine IL-1 and rotavirus VP6 protein by using co-transformation technique. And here we examined the effect of oral administration to mice with combinations of vaccine and cytokine, using the transgenic strawberries and/or the transgenic VP6 potato mentioned above.
BALB/c (8 week-old) mice were spontaneously fed with the transgenic plants once in a week, three times. After 1 week of the last(3rd) administration, those mice were challenged using rotavirus EW strain (murine group A) with dose of 10^5 TCID50.
Virus antigen and anti-virus antibody in blood and feces of the challenged mice were examined. Secretory IgA was detected in feces of VP6 administrated mice. On the other hand, there was no significant difference in immune-markers among the hlFN strawberry, the IL-1 strawberry and non lransgenic strawberry administrated groups ;

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (7 results)

All 2005 2004 Other

All Journal Article (5 results) Book (2 results)

  • [Journal Article] Ability of Orally Administered IFN-α containing Transgenic Potato Extracts to Inhibit Listeria monocytogenes Infection.2005

    • Author(s)
      Kenji Ohya, Takeshi Matsumura, Noriko Itchoda, Kazuhiko Ohashi, Misao Onuma, Chiro Sugimoto
    • Journal Title

      Journal of Interferon & Cytokine Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increase of Antibody Titer against Leucocytozoon caulleryi by Oral Administration of Recombinant R7 Antigen2005

    • Author(s)
      Akira Ito, Tohru Gotand, Shigeki Kobayashi, Katsumi Kume, Chihiro Sugimoto, Takeshi Matsumura
    • Journal Title

      J.Vet.Med.Sci 67(2)

      Pages: 211-213

    • NAID

      110003963706

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increase of Antibody Titer against Leucocytozoon caulleryj by Oral Administration of Recombinant R7 Antigen.2005

    • Author(s)
      Akira Ito, Tohru Gotand, Shigeki Kobayashi, Katsumi Kume, Chihiro Sugimoto, Takeshi Matsumura
    • Journal Title

      J.Vet.Med.Sci 67(2)

      Pages: 211-213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Mammlian Cytokines Produced in pints.2004

    • Author(s)
      Chihiro Sugimoto, Takeshi Matsumura
    • Journal Title

      Encyclopedia of Plant and Crop Science (MRCEL DEKKER, Inc) (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ability of Orally Administered IFN-α containing Transgenic Potato Extracts to Inhibit Ldsteria monocytogenes Infection.

    • Author(s)
      Kenji Ohya, Takeshi Matsumura.Noriko Itchoda, Kazuhiko Ohashi, Misao Onuma, Chiro Sugimoto
    • Journal Title

      Journal of Interferon & Cytokine Research (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] ENCYCLOPEDIA OF PLANT & CROP SCIENCE2004

    • Author(s)
      松村健, 杉本千尋
    • Total Pages
      1329
    • Publisher
      MRCEL DEKKER, INC
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] ENCYCLOPEDIA OF PLANT & CROP SCIENCE2004

    • Author(s)
      松村健, 杉本千尋
    • Total Pages
      1329
    • Publisher
      MARCEL DEKKER, INC
    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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