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Oncogenesis of retrovirus induced-glioma and the development of animal model

Research Project

Project/Area Number 15380207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OCHIAI Kenji  Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院獣医学研究科, 助教授 (80214162)

Co-Investigator(Kenkyū-buntansha) OHASHI Kazuhiko  Hokkaido University, Graduate School of Veterinary Medicine, Associate Professor, 大学院獣医学研究科, 助教授 (90250498)
ONO Etsuro  Tottori University, Department of Agriculture, Professor, 農学部, 教授 (00160903)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥6,200,000 (Direct Cost: ¥6,200,000)
Keywordsavian leukosis virus (ALV) / astrocytoma / oncogenesis / fowl / glioma / nervous system / transgenic mice / トリ / 中枢神経系腫瘍 / レトロウイルス
Research Abstract

The cause and pathological features of so-called fowl glioma (astrocytoma) had not been determined. We have suggested that the cause is a subgroup A of avian leukosis virus (ALV). The subject of this research project is to clarify the pathogenicity of fowl glioma-inducing virus (FGV) and to analyze the molecular biological role of env gene and LTR in the neuropathogenicity. At first, chickens experimentally infected with FGV were pathologically examined. Whereas other isolated ALVs generally induce hematopoietic neoplasms, FGV was likely to replicate especially in brain and heart and induced nonsuppurative encephalitis and myocarditis, perineurioma, cerebellar hypoplasia as well as astrocytoma in nervous system. Genome sequence analysis suggested FGV to be combined with several virus strains. The U3 region of the LTR had a few deletions and several point mutations compared to that of other ALVs (EMBL, GenBank, and DDBJ nucleotide sequence databases under the accession number AB112960). The promoter activity of LTR also showed subtle differences, which may be related to the induction of glial neoplasm. In addition, histopathology of the 51 affected brains suggested the presence of a histological variant composed of diffuse proliferation of astrocytes and indicated that the disease entity should be named "retrovirus-induced astrocytoma" of chickens. To examine the promoter activity of FGV LTR in vivo, 13 lines of transgenic mice expressing a transgene under the control of the LTR promoter were generated and the tissue specificity of the expression was analyzed by monitoring mRNA in each line using the reverse transcription-polymerase chain reaction. The results indicated that the FGV LTR promoter was capable of driving a stable transgene expression especially in the central nervous system and testis although the LTR acted as a panspecific promoter in vivo.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (9 results)

All 2006 2005 2004 2003 Other

All Journal Article (8 results) Publications (1 results)

  • [Journal Article] Cerebellar hypoplasia associated with an avian leucosis virus inducing fowl glioma2006

    • Author(s)
      Toyoda, T., Ochiai, K.et al.
    • Journal Title

      Vet. Pathol. 43(3)

      Pages: 294-301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cerebellar hypoplasia associated with an avian leukosis virus inducing fowl glioma2006

    • Author(s)
      Toyoda, T, Ochiai, K. et al.
    • Journal Title

      Veterinary Pathology In press

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Multiple perineuriomas in chicken (Gallus gallus domesticus)2005

    • Author(s)
      Toyoda, T., Ochiai, K.et al.
    • Journal Title

      Vet. Pathol. 42(2)

      Pages: 176-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Peripheral nerve sheath tumor (perineurioma) in Japanese bantam (Gallus gallus domesticus) inoculated with an avian leukosis virus2005

    • Author(s)
      Toyoda, T, Ochiai, K. et al.
    • Journal Title

      Veterinary Pathology 42(2)

      Pages: 176-183

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Peripheral nerve sheath tumor(perineurioma) in Japanese bantam(Gallus gallus domesticus) inoculated with an avian leukosis virus2005

    • Author(s)
      Toyoda, T, Ochiai, K. et al.
    • Journal Title

      Veterinary Pathology In press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genome sequence analysis of the avian retrovirus causing so-called fowl glioma and the promoter avtivity of the long terminal repeat2004

    • Author(s)
      Tomioka, Y., Ochiai, K.et al.
    • Journal Title

      J. Gen. Virol. 85(3)

      Pages: 647-652

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genome sequence analysis of the avian retrovirus causing so-called fowl glioma and the promoter activity of the long terminal repeat2004

    • Author(s)
      Tomioka, Y., Ochiai, K.et al.
    • Journal Title

      J. Gen. Virol. 85(3)

      Pages: 647-652

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In ovo infection with an avian leucosis virus causing fowl glioma : viral distribution and pathogenesis2003

    • Author(s)
      Tomioka, Y., Ochiai, K.et al.
    • Journal Title

      Avian Pathology 32(6)

      Pages: 617-624

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Tomioka, Y., Ochiai, K., et al.: "In ovo infection with an avian leucosis virus causing fowl glioma : viral distribution and pathogenesis"Avian Pathology. 32(6). 617-624 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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