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Advanced Utilization of Rhizosphere Functions for Phytoremediation of Heavy Metal Contaminated Soils.

Research Project

Project/Area Number 15380223
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionKochi University

Principal Investigator

IWASAKI Kozo  Kochi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (40193718)

Co-Investigator(Kenkyū-buntansha) KANG Yumei  Kochi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70284429)
TANAKA Sota  Kochi University, Graduate School of Kuroshio Science, Assistant, 大学院・黒潮圏海洋科学研究科, 助手 (10304669)
SAKURAI Katsutoshi  Kochi University, Faculty of Agriculture, Professor, 農学部, 教授 (90192088)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2003: ¥10,700,000 (Direct Cost: ¥10,700,000)
KeywordsCadmium / Zinc / Arsenic / Arabis flagellosa / Athyrium yokoscense / Rice plants / Accumulating plants / Rhizosphere / ヒマワリ / ファイトレメディエーション / 重金属汚染 / 生分解性キレート剤 / 土壌微生物 / 銅
Research Abstract

To develop effective and environmentally clean phytoremediation technologies, basic researches were conducted concerning about 1)biodegradable chelate-assisted phytoremediation, 2)survey of metal accumulating plants and the mechanism of their metal accumulation, and 3)utilization of soil amendments for reducing As movement from rhizosphere to crop shoots. The main results were listed below :
1.Addition of biodegradable chelating agents (S,S-ethylenediamine-N,N'-disuccinic acid, EDDS) to Cu contaminated soils significantly increased Cu uptake by brown mustard, compared with the plants grown without EDDS. Environmental risks, such as leaching of Cu and Cu-chelate and the impact on the soil microorganisms were smaller than the case of EDTA addition. However, biodegradable chelate-assisted phytoremediation should be carried out under isolated condition because complete reduction of leaching of Cu and chelating agents during plant cultivation was difficult.
2.Soil and plant survey around the … More closed silver mine at Ikuno-cho, Hyogo Prefecture, Japan revealed that Arabis flagellosa is a new Zn and Cd hyperaccumulating plant. It was also found that well-known heavy metal accumulating fern, Athyrium yokoscense can accumulate As and heavy metals, simultaneously. More than 80 and 55% of Zn and Cd in the leaves of Arabis flagellosa were soluble forms, respectively. It was suggested that malic acid contributed to detoxify high concentrations of Zn in the leaves. When the plant was treated with high Cd, increase of SH compounds such as phytochelatins in the roots were confirmed, which suggested the detoxification of Cd by these compounds.
3.As toxicity symptoms and As uptake of sunflower grown on As contaminated soils were significantly reduced by the application of arbuscular mycorrhizal inoculum, compared with the plants supplied with P fertilizers. On the other hand, application of amorphous Fe hydroxides to paddy soil significantly reduced As uptake by rice plants irrigated with As contaminated water. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (41 results)

All 2005 2004 2003 Other

All Journal Article (35 results) Book (3 results) Patent(Industrial Property Rights) (1 results) Publications (2 results)

  • [Journal Article] Influence of chelating agent addition on copper distribution and microbial activity in the soil and copper uptake by brown mustard (Brassica juncea).2005

    • Author(s)
      Venecio Ultra Jr.
    • Journal Title

      Soil Sci. Plant Nutr. 51(2)

      Pages: 193-202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Arsenic Transformation and Microbial Community Structure in the Rhizosphere of Rice Irrigated with As-Contaminated Water.2005

    • Author(s)
      V.U.Ultra, Jr.
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection, Eds. C.J.Li et al., Tsinghua Univ. Press, Beijing China

      Pages: 696-697

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of Cu, Zn, Cd, and Pb in plants growing on the soil near an old silver mine in Hyogo Prefecture, Japan2005

    • Author(s)
      Y.Kang
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection, Eds. C.J.Li et al., Tsinghua Univ. Press, Beijing China

      Pages: 752-753

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous Phytoremediation of Arsenic and Heavy Metals in Contaminated Soils with Athyrium yokoscense.2005

    • Author(s)
      V.K.Tran
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection, Eds. C.J.Li et al., Tsinghua Univ. Press, Beijing China

      Pages: 694-695

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] スズシロソウによる亜鉛、カドミウム吸収2005

    • Author(s)
      小郷みつ子
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 73-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 鉱山周辺に生息するヘビノネゴザ・スズシロソウ群落のヒ素・重金属吸収と土壌の関係2005

    • Author(s)
      福井貴博
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 171-171

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] ヒ素添加土壌と鉱山土壌で栽培したヘビノネゴザのヒ素・重金属集積特性2005

    • Author(s)
      康 峪梅
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 184-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Arbuscular mycorrhiza and phosphorus application alleviate arsenic toxicity of sunflower.2005

    • Author(s)
      Ultra Jr. Venecio
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 38-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Concentrations and chemical forms of heavy metals in urban soils of Shanghai, China.2005

    • Author(s)
      Zonghui Chen
    • Journal Title

      日本土壌肥料学会関西支部会講演要旨集 101

      Pages: 25-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Influence of chelating agent addition on copper distribution and microbial activity in the soil and copper uptake by brown mustard (Brassica juncea).2005

    • Author(s)
      Venecio Ultra Jr. et al.
    • Journal Title

      Soil Sci. Plant Nutl 51(2)

      Pages: 193-202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Arsenic Transformation and Microbial Community Structure in the Rhizosphere of Rice Irrigated with As-Contaminated Water.2005

    • Author(s)
      V.U.Ultra, Jr.et al.
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection (Eds. C.J.Li et al.)(Tsinghua Univ. Press) Beijing China

      Pages: 696-697

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of Cu, Zn, Cd, and Pb in plants growing on the soil near an old silver mine in Hyogo Prefecture, Japan.2005

    • Author(s)
      Y.Kang et al.
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection (Eds. C.J.Li et al.)(Tsinghua Univ. Press) Beijing China

      Pages: 752-753

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simultaneous Phytoremediation of Arsenic and Heavy Metals in Contaminated Soils with Athyrium yokoscense.2005

    • Author(s)
      V.K.Tran et al.
    • Journal Title

      Plant Nutrition for Food Security, Human Health and Environmental Protection (Eds. C.J.Li et al.)(Tsinghua Univ. Press) Beijing China

      Pages: 694-695

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Arbuscular mycorrhiza and phosphorus application alleviate arsenic toxicity of sunflower.2005

    • Author(s)
      Ultra Jr.Venecio et al.
    • Journal Title

      Abstracts of the Annual Meeting, Japanese Society of Soil Science and Plant Nutrition 51

      Pages: 38-38

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Concentrations and chemical forms of heavy metals in urban soils of Shanghai, China.2005

    • Author(s)
      Zonghui Chen et al.
    • Journal Title

      Abstracts of the Annual Meeting, Kansai Branch of Japanese Society of Soil Science and Plant Nutrition 101

      Pages: 25-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Influence of chelating agent addition on copper distribution and microbial activity in soil and copper uptake by brown mustard (Brassica juncea).2005

    • Author(s)
      Venecio Ultra, Jr.
    • Journal Title

      Soil Science and Plant Nutrition 51・2

      Pages: 193-202

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Arsenic Transformation and Microbial Community Structure in the Rhizosphere of Rice Irrigated with As-Contaminated Water.2005

    • Author(s)
      V.U.Ultra, Jr.
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection. (Tsinghua Univ. Press, Beijing China)

      Pages: 696-697

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Accumulation of Cu, Zn, Cd, and Pb in plants growing on the soil near an old silver mine in Hyogo Prefecture, Japan.2005

    • Author(s)
      Y.Kang
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection. (Tsinghua Univ. Press, Beijing China)

      Pages: 752-753

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simultaneous Phytoremediation of Arsenic and Heavy Metals in Contaminated Soils with Athyrium yokoscense.2005

    • Author(s)
      V.K.Tran
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection. (Tsinghua Univ. Press, Beijing China)

      Pages: 694-695

    • Related Report
      2005 Annual Research Report
  • [Journal Article] スズシロソウによる亜鉛,カドミウム吸収2005

    • Author(s)
      小郷みつ子
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 73-73

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Arbuscular mycorrhiza and phosphorus application alleviate arsenic toxicity of sunflower.2005

    • Author(s)
      Ultra Jr.Venecio
    • Journal Title

      日本土壌肥料学会講演要旨集 51

      Pages: 38-38

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Influence of chelating agent addition on copper distribution and microbial activity in soil and copper uptake by brown mustard (Brassica juncea).2005

    • Author(s)
      Venecio Ultra, Jr.
    • Journal Title

      Soil Science and Plant Nutrition 51・2(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Arsenic Transformation and Microbial Community Structure in the Rhizosphere of Rice Irrigated with As-Contaminated Water.2005

    • Author(s)
      V.U.Ultra, Jr.
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Accumulation of Cu, Zn, Cd, and Pb in plants growing on the soil near an old silver mine in Hyogo Prefecture, Japan.2005

    • Author(s)
      Y.Kang
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Simultaneous Phytoremediation of Arsenic and Heavy Metals in Contaminated Soils with Athyrium yokoscense.2005

    • Author(s)
      V.K.Tran
    • Journal Title

      "Plant Nutrition" for Food Security, Human Health and Environmental Protection (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chemical forms of arsenic in the rhizosphere soil of rice irrigated with arsenic contaminated water.2004

    • Author(s)
      Venecio Ultra Jr. UY
    • Journal Title

      日本土壌肥料学会関西支部会講演要旨集 100

      Pages: 9-9

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] カドミウム汚染土壌に生育させた数種類の植物によるカドミウム吸収2004

    • Author(s)
      佐藤敦彦
    • Journal Title

      日本土壌肥料学会関西支部会講演要旨集 100

      Pages: 10-10

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 兵庫県生野鉱山跡地周辺に生息する植物および土壌の重金属含有率,第1報 土壌断面及び植生の調査2004

    • Author(s)
      康 峪梅
    • Journal Title

      日本土壌肥料学会講演要旨集 50

      Pages: 177-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 兵庫県生野鉱山跡地周辺に生息する植物および土壌の重金属含有率,第2報 集積植物の検索2004

    • Author(s)
      岩崎貢三
    • Journal Title

      日本土壌肥料学会講演要旨集 50

      Pages: 177-177

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chemical forms of arsenic in the rhizosphere soil of rice irrigated with arsenic contaminated water.2004

    • Author(s)
      Venecio Ultra Jr. UY et al.
    • Journal Title

      Abstracts of the Annual Meeting, Kansai Branch of Japanese Society of Soil Science and Plant Nutrition 100

      Pages: 9-9

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 兵庫県生野鉱山跡地周辺に生息する植物及び土壌の重金属含有率第1報 土壌断面及び植生の調査2004

    • Author(s)
      康 峪梅
    • Journal Title

      日本土壌肥料学会講演要旨集 50

      Pages: 117-117

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 兵庫県生野鉱山跡地周辺に生息する植物及び土壌の重金属含有率第2報 集積植物の検索2004

    • Author(s)
      岩崎貢三
    • Journal Title

      日本土壌肥料学会講演要旨集 50

      Pages: 117-117

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Chemical forms of arsenic in the rhizosphere soil of rice irrigated with arsenic contaminated water.2004

    • Author(s)
      Venecio Ultra, Jr.UY
    • Journal Title

      日本土壌肥料学会関西支部講演会要旨集 100

      Pages: 9-9

    • Related Report
      2004 Annual Research Report
  • [Journal Article] キレート剤の施用がコンフリーのCd吸収に及ぼす影響2003

    • Author(s)
      佐藤敦彦
    • Journal Title

      日本土壌肥料学会関西支部会講演要旨集 99

      Pages: 7-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 生分解性キレート剤を利用した銅汚染土壌のファイトレメディエーション2003

    • Author(s)
      矢野敦子
    • Journal Title

      日本土壌肥料学会講演要旨集 49

      Pages: 117-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 5.3栄養生理と環境,「新 農業気象・環境学」2005

    • Author(s)
      岩崎貢三
    • Total Pages
      209
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 新農業気象・環境学2005

    • Author(s)
      岩崎貢三
    • Total Pages
      12
    • Publisher
      朝倉書店
    • Related Report
      2005 Annual Research Report
  • [Book] 新 農業気象・環境学2005

    • Author(s)
      岩崎貢三
    • Publisher
      朝倉書店(印刷中)
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 土壌中重金属の除去及び回収方法2004

    • Inventor(s)
      櫻井克年, 岩崎貢三, 康 峪梅, 大嶋秀俊, 田中壮太, 相川良雄
    • Industrial Property Rights Holder
      国立大学法人高知大学, 三菱マテリアル株式会社
    • Industrial Property Number
      2004-232408
    • Filing Date
      2004-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Publications] 矢野 敦子: "生分解性キレート剤を利用した銅汚染土壌のファイトレメディエーション"日本土壌肥料学会講演要旨集. 49. 177 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 佐藤 敦彦: "キレート剤の施用がコンフリーのCd吸収に及ぼす影響"日本土壌肥料学会関西支部講演会要旨集. 99. 7 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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