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Integration and visualization of genome information in Lepidoptera

Research Project

Project/Area Number 15380227
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionHOKKAIDO UNIVERISTY

Principal Investigator

SAHARA Ken  Hokkaido Univ., Grad.School of Agr., Assistant Prof., 大学院・農学研究科, 助手 (30241368)

Co-Investigator(Kenkyū-buntansha) YASUKOCHI Yuji  National Inst. Agrobiological Sciences, Senior Researcher, ゲノム研究グループ, 主任研究官 (50355723)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2003: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsBombyx mori / BAC-FISH / GISH / contig / linkage map / chromosome identification / karyotyping / Lepidoptera / BAC / FISH / RAPD地図 / コールドスポット / 性染色体 / 突然変異地図
Research Abstract

We have developed Bombyx mori sex chromosome identification method and first BAC-FISH system in Lepidoptera by means of W-BAC selected by female specific STSs in 2003. The former method has been applied to resolve multiple sex chromosome systems in 2005. Modified BAC-FISH procedure using genetically mapped B.mori BACs as probes allow us to identify each 28 bivalents. Furthermore, the combination of two to four either green or red fluorescence-labeled probes per chromosome enables us to recognize unequivocally each of the 28 bivalents of the B.mori karyotype by its labeling pattern, which have been impossible for more than 80 years. Through the research we have accomplished 2nd generation molecular linkage map including 512 BAC contigs, which correspond to most unique genes published on the web. These may feasibly facilitate the map-based cloning the genes corresponding to mutants.
The BAC-FISH signal localities coincide with their mapping loci detected by genetic analysis with a few exceptions. Since the discrepancy may be caused by mistakes of three-point analysis, we had better re-calculate the results.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (44 results)

All 2006 2005 2004 2003 Other

All Journal Article (38 results) Publications (6 results)

  • [Journal Article] Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.2006

    • Author(s)
      Niimi T et al.
    • Journal Title

      Genome -(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phylogeny of the sex determining gene Sex-lethal in insects.2006

    • Author(s)
      Traut W et al.
    • Journal Title

      Genome -(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene2006

    • Author(s)
      Niimi T, Sahara K, Oshima H, Yasukochi Y, Ikeo K, Traut W
    • Journal Title

      Genome (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phylogeny of the sex determining gene Sex-lethal in insects.2006

    • Author(s)
      Traut W, Niimi T, Ikeo K, Sahara K
    • Journal Title

      Genome (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular cloning and chromosomal localization of the Bombyx Sex-lethal gene.2006

    • Author(s)
      Niimi T et al.
    • Journal Title

      Genome (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phylogeny of the sex determining gene Sex-lethal in insects.2006

    • Author(s)
      Traut W et al.
    • Journal Title

      Genome (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simple sequence repeat-based consensus linkage map of Bombyx mori., 102,16303-16308.2005

    • Author(s)
      Miao XX et al.
    • Journal Title

      Proceeding of the National Academy of Sciences USA 102

      Pages: 16303-16308

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n=28).2005

    • Author(s)
      Yasukochi Y et al.
    • Journal Title

      Genome 48

      Pages: 626-629

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Retrotransposable elements on the W chromosome of the silkworm, Bombyx mori.2005

    • Author(s)
      Abe H et al.
    • Journal Title

      Cytogenetic and Genome Research 110

      Pages: 144-151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG)n telomeric probe in some species of Lepidoptera.2005

    • Author(s)
      Yoshido A et al.
    • Journal Title

      Chromosoma 114

      Pages: 193-202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The Bombyx mori karyotype and the assignment of linkage groups.2005

    • Author(s)
      Yoshido A et al.
    • Journal Title

      Genetics 170

      Pages: 675-685

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori,2005

    • Author(s)
      Abe H et al.
    • Journal Title

      Insect Molecular Biology 14

      Pages: 339-352

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simple sequence repeat-based consensus linkage map of Bombyx mori.2005

    • Author(s)
      Miao XX, Xub SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng P, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang X, Huang W, Yasukochi Y, Sugasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102

      Pages: 16303-16308

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n=28).2005

    • Author(s)
      Yasukochi Y.Banno Y, Yamamoto K, Goldsmith MR, Fujii H
    • Journal Title

      Genome 48

      Pages: 626-629

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Retrotransposable elements on the W chromosome of the silkworm, Bombyx mori.2005

    • Author(s)
      Abe H, Mita K, Yasukochi Y, Oshiki T, Shimada T
    • Journal Title

      Cytogenet, Genome Res. 110

      Pages: 144-151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG)n telomeric probe in some species of Lepidoptera.2005

    • Author(s)
      Yoshido A, Marec F, Sahara K
    • Journal Title

      Chromosoma 114

      Pages: 193-202

    • NAID

      120000956785

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori.2005

    • Author(s)
      Abe H, Seki M, Ohbayashi F, Tanaka N, Yamashita J, Fujii T, Yokoyama T, Takahashi M, Banno Y, Sahara K, Yoshido A, Ihara J, Yasukochi Y, Mita K, Ajimura M, Suzuki MG, Oshiki T, Shimada T
    • Journal Title

      Insect Mol.Biol. 14

      Pages: 339-352

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Bombyx mori karyotype and the assignment of linkage groups.2005

    • Author(s)
      Yoshido A, Bando H, Yasukochi Y, Sahara K
    • Journal Title

      Genetics 170

      Pages: 675-685

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Simple sequence repeat-based consensus linkage map of Bombyx mori.2005

    • Author(s)
      Miao XX et al.
    • Journal Title

      Proceeding of the National Academy of Sciences USA 102

      Pages: 16303-16308

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The Bombyx mori karyotype and the assignment of linkage groups.2005

    • Author(s)
      Yosido A et al.
    • Journal Title

      Genetics (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inbreeding depression on female fecundity by genetic factors retained in small population of a male-haploid social mite.2005

    • Author(s)
      Mori K et al.
    • Journal Title

      Experimental and Applied Acarology (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The sex pheromone receptor in the silkworm moth Bombyx mori.2004

    • Author(s)
      Sakurai T et al.
    • Journal Title

      Proceeding National Academy of Science USA 101

      Pages: 16653-16658

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms.2004

    • Author(s)
      Isobe R et al.
    • Journal Title

      Archives of Virology 149

      Pages: 1931-1940

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Organization of the Hox gene cluster of the silkworm, Bombyx mori : a split of the Hox cluster in a non-Drosophila insect.2004

    • Author(s)
      Yasukochi Y et al.
    • Journal Title

      Development Genes and Evolution 214

      Pages: 606-614

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] cDNA cloning, gene structure, and expression of Broad-Complex (BR-C) genes in the silkworm, Bombyx mori.2004

    • Author(s)
      Ijiro T et al.
    • Journal Title

      Insect Biochemistry and Molecular Biology 34

      Pages: 963-969

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The genome sequence of silkworm, Bomnyx mori.2004

    • Author(s)
      Mita K et al.
    • Journal Title

      DNA Research 11

      Pages: 27-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Organization of the Hox Gene Cluster of the silkworm, Bombyx mori : a split of the Hox cluster in a non-Drosophila insect.2004

    • Author(s)
      Yasukochi Y, Ashakumary LA, Wu C, Yoshido A, Nohata J, Mita K, Sahara K
    • Journal Title

      Dev.Genes Evol. 214

      Pages: 606-614

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification and functional characterization of a sex pheromone receptor in the silkmoth Bombyx mori.2004

    • Author(s)
      Sakurai T, Nakagawa T, Mitsuno H, Mori H, Endo Y, Tanoue S, Yasukochi Y, Touhara K, Nishioka T
    • Journal Title

      Proc.Natl.Acad.Sci.USA 101

      Pages: 16653-16658

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Use of RNAi technology to confer enhanced resistance to BmNPV on transgenic silkworms.2004

    • Author(s)
      Isobe R, Kojima K, Matsuyama T, Quan G-X, Kanda T, Tamura T, Sahara K, Asano S-I, Bando H
    • Journal Title

      Arch.Virol. 149

      Pages: 1931-1940

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] cDNA loning, gene structure, and expression of Broad-Complex (BR-C) genes in the silkworm, Bombyx mori.2004

    • Author(s)
      Ijiro T, Urakawa H, Yasukochi Y, Takeda M, Fujiwara Y
    • Journal Title

      Insect Biochem.Mol.Biol. 34

      Pages: 963-969

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Genome Sequence of Silkworm, Bombyx mori.2004

    • Author(s)
      Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin-I T, Abe H, Shimoda T, Morishita, Sasaki S
    • Journal Title

      DNA Res. 11

      Pages: 27-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The sex pheromone receptor in the silkworm moth Bombyx mori.2004

    • Author(s)
      Sakurai T et al.
    • Journal Title

      Proceeding Natural Academy of Science USA 101

      Pages: 16653-16658

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Organization of the Hox gene cluster of the silkworm, Bombyx mori : A split of the Hox cluster in a non-Drosophila insect.2004

    • Author(s)
      Yasukochi Y et al.
    • Journal Title

      Development Genes and Evolution 214

      Pages: 606-614

    • Related Report
      2004 Annual Research Report
  • [Journal Article] cDNA cloning, gene structure, and expression of Broad-Complex(BR-C) genes in the silkworm, Bombyx mori.2004

    • Author(s)
      Ijiro T et al.
    • Journal Title

      Insect Biochemistry and Molecular Biology 34

      Pages: 963-969

    • Related Report
      2004 Annual Research Report
  • [Journal Article] W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori.2003

    • Author(s)
      Sahara K
    • Journal Title

      Chromosoma 112

      Pages: 48-55

    • NAID

      120000964279

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Moth sex chromatin probed by comparative genomic hybridization (CGH).2003

    • Author(s)
      Sahara K
    • Journal Title

      Genome 46

      Pages: 339-342

    • NAID

      120000958655

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori.2003

    • Author(s)
      Sahara K, Yoshido A, Kawamura N, Ohnuma A, Abe H, Mita K, Oshiki T, Shimoda T, Asano S, Bando H, Yasukochi Y
    • Journal Title

      Chromosoma 112

      Pages: 48-55

    • NAID

      120000964279

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Moth sex chromatin probed by comparative genomic hybridization (CGH).2003

    • Author(s)
      Sahara K, Marec F, Eickhoff U, Traut W
    • Journal Title

      Genome 46

      Pages: 339-342

    • NAID

      120000958655

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Abe H: "Retrotransposable elements on the W chromosome of the silkworm, Bombyx mori"Cytogenetic and Genome Research. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sahara K: "Roles of actin networks in peristaltic squeezing of sperm bundles in Bombyx mori"Journal of Morphology. 259・1. 1-6 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sahara K: "W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori"Chromosoma. 112・1. 48-55 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsuyama T: "Function analysis of an immediate early gene, iel, of Bombyx mori nucleopolyhedrovirus in mammalian cells"Journal of Insect Biotechnology and Sericology. 72・2. 87-94 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sahara K: "Moth sex chromatin probed by comparative genomic hybridization(CGH)"Genome. 46・2. 339-342 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sahara K: "Application of artificial insemination technique to separated eupyrene and apyrene sperm in Bombyx mori"Journal of Experimental Zoology. 297A・2. 196-200 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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