| Project/Area Number |
15390011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
SHIMADA Kazutake Kanazawa University, Graduate School of Natural Science and Technology, Professor, 自然科学研究科, 教授 (90004605)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHI Tatsuya Kanazawa University, Graduate School of Natural Science and Technology, Associate professor, 自然科学研究科, 助教授 (90272963)
三田村 邦子 金沢大学, 自然科学研究科, 助手 (70242526)
本間 誠次郎 帝国臓器製薬メディカル, 受託研究部, 部長
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,700,000 (Direct Cost: ¥15,700,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥13,500,000 (Direct Cost: ¥13,500,000)
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| Keywords | prostate cancer / benign prostatic hyperplasia / bio-marker / testosterone / dihydrotestosterone / deivatization-LC / MS / mass-screening / inhibitor / LC / 電子捕獲型 / エレクトロスプレーイオン化 / MSプローブ / 再燃 / 予後診断 / アンドロゲン / 誘導体化試薬 |
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| Research Abstract |
During the last decade, prostate cancer has been a commonly diagnosed malignancy in males. The research focused on steroids [testosterone (T) and its active form dihydrotestosterone (DHT)] in human prostate or serum, which are the potential bio-markers for diagnosing prostate cancer (PC) or benign prostatic hyperplasia (BPH). We have developed a new derivatization reagent (2-hydrazino-1-methylpyridine) having a quarternary pyridinium moiety for the analysis of these androgens using LC/electrospray ionization-MS. The reagent quantitatively reacted with the above oxo-steroids (T and DHT) and the derivatives provided a 70-1600 fold higher sensitivity compared to intact steroids. The developed method allowed the reproducible quantification of nanogram/gram levels of T and DHT with a 10 mg tissue sample of the prostate. The method was applied to the determination of T and DHT in PC and BPH tissues. DHT was determined in BPH tissue, but not detected in PC tissue. T was not detected in both tissues. These data is useful for establishment of mass screening for these diseases.
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