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Molecular Mechanisms of Toxic Expression Induced by Endocrine Disruptors via AHR

Research Project

Project/Area Number 15390040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

YAMAZAKI Hiroshi  Hokkaido Univ., Grad.School of Pharm.Sci., Asso.Prof., 大学院・薬学研究科, 助教授 (30191274)

Co-Investigator(Kenkyū-buntansha) FUJIEDA Masaki  Hokkaido Univ., Grad.School of Pharm.Sci., Inst., 大学院・薬学研究科, 助手 (00344474)
KAMATAKI Tetsuya  Hokkaido Univ., Grad.School of Pharm.Sci., Prof., 大学院・薬学研究科, 教授 (00009177)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2004: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥9,700,000 (Direct Cost: ¥9,700,000)
KeywordsAHR / PAHs / TCDD / CYP1A1 / CYP1B1 / 個人差
Research Abstract

Polycychc aromatic hydrocarbons (PAHs) which are known as the Environmental Endocrine Disruptors exert the various toxicological effects via aryl hydrocarbon receptor (AHR). However the mechanisms of toxic expression induced by PAHs have not been clarified yet. Therefore, the purpose of this study was to clarify the mechanisms of toxic expression induced by PAHs using differential display method and DNA microarray method. First, total RNA were prepared from the wild type mouse and AHR knock out mouse livers treated. with 3-methylcholanthrene (MC). We found the novel AHR target genes using differential display method and DNA microarray method. Interestingly, the mRNA expressions of the peroxisome prolifilator-activated receptor (PPAR) α target genes were suppressed by PAH treatment through AHR. We also demonstrated that AHR inhibited PPARα signals through the suppression of its heterodimeric partner, retinoid X receptor (RXR)α. The genes responsible for the fatty acid metabolism were regulated by PPARα. Therefore, it was suggested that the suppression of the PPARα signal was important for the occurrence of fatty liver.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (22 results)

All 2004 Other

All Journal Article (15 results) Publications (7 results)

  • [Journal Article] Identification of a novel polymorphic enhancer of the human CYP3A4 gene2004

    • Author(s)
      Keiko Matsumura et al.
    • Journal Title

      Mol.Pharmacol. 65

      Pages: 326-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of CYP2A6 Genetic Polymorphisms as Determinants of Smoking Behavior and Tobacco-related Lung Cancer Risk in Male Japanese Smokers.2004

    • Author(s)
      Masaki Fujieda et al.
    • Journal Title

      Carcinogenesis 25

      Pages: 2451-2458

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of the dietary supplements, activated charcoal and copper chlorophyllin, on urinary excretion of trimethylamine in Japanese trimethylaminuria patients2004

    • Author(s)
      Hiroshi Yamazaki et al.
    • Journal Title

      Life Sci. 74

      Pages: 2739-2747

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] CYP3A5 contributes significantly to CYP3A-mediated drug oxidations in liver microsomes from Japanese subjects2004

    • Author(s)
      Satoshi Yamaori et al.
    • Journal Title

      Drug Metab.Pharmacokinet. 19

      Pages: 120-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Uridine diphosphate sugar-selective conjugation of an aldose reductase inhibitor (AS-3201) by UDP-glucuronosyltransferase 2B subfamily in human liver microsomes2004

    • Author(s)
      Kenji Toide et al.
    • Journal Title

      Biochem.Pharmacol. 67

      Pages: 1269-1278

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of ten strains of genetically engineered Salmonella typhimurium TA1538 each co-expressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase sensitive to various promutagens2004

    • Author(s)
      Yoshiyuki Yamazaki et al.
    • Journal Title

      Mutat.Res. 562

      Pages: 151-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a novel polymorphic enhancer of the human CYP3A4 gene.2004

    • Author(s)
      Keiko Matsumura et al.
    • Journal Title

      Mol.Pharmacol 65

      Pages: 326-334

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of the dietary supplements, activated charcoal and copper chlorophyllin, on urinary excretion of trimethylamine in Japanese trimethylaminuria patients.2004

    • Author(s)
      Hiroshi Yamazaki et al.
    • Journal Title

      Life Sci. 74 2

      Pages: 2739-2747

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] CYP3A5 contributes significantly to CYP3A-mediated drug oxidations in liver microsomes from Japanese subjects.2004

    • Author(s)
      Satoshi Yamaori et al.
    • Journal Title

      Drug Metab.Pharmacokinet. 19

      Pages: 120-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of a novel polymorphic enhancer of the human CYP3A4 gene2004

    • Author(s)
      Keiko Matsumura et al.
    • Journal Title

      Mol.Pharmacol. 65(2)

      Pages: 326-334

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of the dietary supplements, activated charcoal and copper chlorophyllin, on urinary excretion of trimethylamine in Japanese trimethylaminuria patients2004

    • Author(s)
      Hiroshi Yamazaki et al.
    • Journal Title

      Life Sci. 74(22)

      Pages: 2739-2747

    • Related Report
      2004 Annual Research Report
  • [Journal Article] CYP3A5 contributes significantly to CYP3A-mediated drug oxidations in liver microsomes from Japanese subjects2004

    • Author(s)
      Satoshi Yamaori et al.
    • Journal Title

      Drug Metab.Pharmacokinet. 19(2)

      Pages: 120-129

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Uridine diphosphate sugar-selective conjugation of an aldose reductase inhibitor(AS-3201) by UDP-glucuronosyltransferase 2B subfamily in human liver microsomes2004

    • Author(s)
      Kenji Toide et al.
    • Journal Title

      Biochem.Pharmacol. 67

      Pages: 1269-1278

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Establishment of ten strains of genetically engineered Salmonella typhimurium TA1538 each co-expressing a form of human cytochrome P450 with NADPH-cytochrome P450 reductase sensitive to various promutagens2004

    • Author(s)
      Yoshiyuki Yamazaki et al.
    • Journal Title

      Mutat.Res. 562(1-2)

      Pages: 151-162

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Evaluation of CYP2A6 Genetic Polymorphisms as Determinants of Smoking Behavior and Tobacco-related Lung Cancer Risk in Male Japanese Smokers2004

    • Author(s)
      Masaki Fujieda et al.
    • Journal Title

      Carcinogenesis 25(12)

      Pages: 2451-2458

    • Related Report
      2004 Annual Research Report
  • [Publications] Toide K, Yamazaki H, et al.: "Aryl hydrocarbon hydroxylase represents CYP1B1, and not CYP1A1, in human freshly isolated white cells"Cancer Epidem Biomarker Prev. 12. 219-222 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Toide K, Yamazaki H, Kamataki T: "Reply : Correspondence re : K.Toide et al."Cancer Epidem Biomarker Prev. 12. 1118 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Saito T, et al.: "Novel nonsynonymous polymorphisms of the CYP1A1 gene in Japanese"Drug Metab Pharmacokin. 18. 218-221 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamaori S, Yamazaki H, et al.: "Effects of cytochrome b5 on drug oxidation activities of human cytochrome P450 (CYP) 3As"Biochem Pharmacol. 66. 2333-2340 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takeuchi H, et al.: "Pretreatment with 8-methoxypsoralen, a potent human CYP2A6 inhibitor, strongly inhibits lung tumorigenesis induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in female A/J mice"Cancer Res. 63. 7571-7583 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kiyotani K, Yamazaki H, et al.: "Decreased coumarin 7-hydroxylase activities and CYP2A6 expression levels in humans caused by genetic polymorphism in CYP2A6 promoter region(CYP2A6*9)"Pharmacogenetics. 13. 689-695 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 山崎浩文, 藤枝正輝, 鎌滝哲也: "「分子予防環境医学」薬物・外来異物に対する生体の代謝機構"本の泉社,分子予防環境医学研究会編. 20 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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