| Project/Area Number |
15390042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TAKEDA Ken Tokyo University of Science, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (80054013)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Seiichi Oita University of Nursing and Health Sciences, Assistant Professor, 講師 (40360060)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | diesel exhaust / in utero exposure / central nerve system / male reproductive system / accessory reproductive gland / testosterone / multi-nuclear giant cells / spontaneous motor activity / Xist遺伝子 / 脳内伝達物質 / 雄性生殖器 / 受動的回避行動 / 組織学的解析 / 脳組織 / Caspase 3 / 血管周囲細胞 / 細胞室内顆粒 |
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| Research Abstract |
The effects of in utero exposure to diesel exhaust (DE) on the mouse male reproductive system and central nerve system were examined. Various markers that appear in pups were assessed. Pregnant ICR mice inhaled DE at DEP concentrations of 0.3, 1.0, or 3.0 mg DEP/m3 or clean air, as the control, on days 2-16 days post coitum. On postnatal day (PND) 28, the weight of the testes and accessory glands, and testosterone concentration in serum were significantly higher in the DE-exposed male pups. Testosterone concentration correlated significantly (P<0.01) with the expression of steroidogenic enzyme mRNAs, weight of the testes and male reproductive accessory glands, and daily sperm production. A spontaneous motor activity was changed intentionally, and the change of the dopamine, noradrenalin and the serotonin systems was admitted in the brain.
These findings indicate that early stage mouse embryo exposure to DE affects male genital system and the cranial nerve system after birth.
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