| Project/Area Number |
15390048
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAKURA Yoshinobu Kyoto Univ., Grad.Sch.Pharm.Sci., Professor, 薬学研究科, 教授 (30171432)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Makiya Kyoto Univ., Grad.Sch.Pharm.Sci., Associate Professor, 薬学研究科, 助教授 (40273437)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2004: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2003: ¥9,800,000 (Direct Cost: ¥9,800,000)
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| Keywords | DNA vaccination / Dendritic cell / Plasmid DNA / Delivery / Cytotoxic T cell / Intracellular trafficking / Electroporation / Intradermal injection / DNAキャリアー / 局所投与 / 抗原提示細胞 / 抗体産生 / 免疫応答 |
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| Research Abstract |
DNA vaccination, which can induce humoral immunity as well as cellular immunity, is a promising approach for treatment of various infectious diseases and tumors. However, its optimization is required to improve the efficacy of this strategy. In particular, delivery to the dendritic cells is one of the most important factors since they play a main role in induction immunity following DNA vaccination. The purpose of this project was to establish the efficient delivery methods to dendritic cells which can optimize the therapeutic effect of DNA vaccine. We found that complex formation between plasmid DNA and cationic macromolecules could enhance antigen specific immune responses by DNA vaccination with intradermal injection. Furthermore, we developed novel plasmid vectors which can control the intracellular trafficking of antigen expressed. We found that intradermal injection of the vector in combination with electroporation could effectively induce antigen specific cytotoxic T cells. These findings would be useful for optimization of DNA vaccination approach.
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