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Analysis of functional connection of the dorsomedial hypothalamic nucleus for themoregulation

Research Project

Project/Area Number 15390072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionWaseda University

Principal Investigator

KANOSUE Kazuyuki  Waseda University, School of Sport Sciences, Professor, スポーツ科学学術院, 教授 (50127213)

Co-Investigator(Kenkyū-buntansha) NAGASHIMA Kei  Waseda University, School of Human Sciences, Associate Professor, 人間科学学術院, 助教授 (40275194)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,200,000 (Direct Cost: ¥15,200,000)
Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2003: ¥9,300,000 (Direct Cost: ¥9,300,000)
KeywordsHypothalamus / Thermoregulation / Dorsomedial nucleus / Non-shivering thermogenesis / Central Grey matter / 中脳中心灰白室
Research Abstract

The preoptic area(POA) in the hypothalamus occupies a crucial position in the neuronal circuit for thermoregulation. Recently, the dorsomedial hypothalamus(DMH) was reported to be involved in the control of heat production. Therefore, in the present study, we investigated the brain areas that had functional afferent connections with the dorsomedial hypothalamus(DMH) for thermoregulation in rats, using immuno-histochemical analyses of a retrograde tracer, cholera toxin-b(CTb), injected into the DMH and Fos expression in response to warm (33℃) or cold (10℃) exposure. A double-labeled cell was defined as the neuron had functional connection with the DMH in relation to thermoregulation. In the warm-exposed rats, double-labeled cells were obtained only in the median preoptic area(MnPO). In the cold exposed rats, double-labeled cells were obtained in the anterior hypothalamus(AH), especially in its medial and ventral parts. The results in the Fos and CTb studies raised a possibility that the DMH neurons would be inhibited by the MnPO neurons, and CTb-IR neurons in the MnPO would be GABAergic and directly regulate activity of the DMH neurons. To examine this possibility, we further investigated an expression of mRNA for GAD67,a marker for GABAergic neuron, in CTB-IR MnPO neuron. We found that 79.6% of CTb-IR cells in the MnPO exhibited signals for GAD67 mRNA. These results suggest that the neurons in the POA, especially in the MnPO, receive warm inputs from the skin and send inhibitory signals, via GABAergic synapses, to the DMH to suppress heat production, and the neurons in the AH receive cold signals from the skin and send excitatory signals to the DMH, to activate heat production.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 2003 Other

All Journal Article (20 results) Publications (4 results)

  • [Journal Article] The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice.2005

    • Author(s)
      Nagashima K et al.
    • Journal Title

      Am J Physiol 288(1)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fos activation in hypothalamic neurons during cold or warm exposure : Projections to periaqueductal gray matter.2005

    • Author(s)
      Yoshida K et al.
    • Journal Title

      Neuroscience (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice.2005

    • Author(s)
      Nagashima, K., K.Matsue, M.Konishi, C.Iidaka, K.Miyazaki, N.Ishida, K.Kanosue
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol. 288(1)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The median preoptic nucleus is involved in the activation of heat-escape/cold-seeking behaviour during systemic salt loading in rats.2005

    • Author(s)
      Konishi, M., K.Kanosue, K.Nagashima
    • Journal Title

      J.Physiol. (being submitted.)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fos activation in hypothalamic neurons during cold or warm exposure : Projections to periaqueductal gray matter.2005

    • Author(s)
      Yoshida, K., M.Konishi, K.Nagashima, C.B.Saper, K.Kanosue
    • Journal Title

      Neuroscience (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Brain activation by thermal stimulation in humans studied with fMRI.2005

    • Author(s)
      Yagishita, T., Sadato, N., Okada, T., Taniguchi, A., Konishi, M., Nagashima, K., Yonekura, Y., Kanosue, K.
    • Journal Title

      Environmental Ergonomics(Eds.Y.Tochihara and T.Ohnaka)(Elsevier)

      Pages: 17-20

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice.2005

    • Author(s)
      Nagashima K et al.
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol. 288(1)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 体温調節のメカニズム2004

    • Author(s)
      彼末一之等
    • Journal Title

      体育の科学 54

      Pages: 764-768

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 体温調節のメカニズム2004

    • Author(s)
      彼末一之, 永島 計, 谷口あき
    • Journal Title

      体育の科学 54

      Pages: 764-768

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibitory projection from the preoptic area to the medullary raphe for the control of thremoregulatory non-shivering thermogenesis in rats.2003

    • Author(s)
      A.Taniguchi et al.
    • Journal Title

      Brain Research 966

      Pages: 103-109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Systemic salt loading suppresses heat production and thermoregulatory behaviour in rats during cold exposure.2003

    • Author(s)
      M.Konishi et al.
    • Journal Title

      J.Physiol. 551

      Pages: 713-720

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Brain regions expressing Fos by thermoregulatory behavior in rats.2003

    • Author(s)
      M.Maruyama et al.
    • Journal Title

      Am.J.Physiol. 285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neurones of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue express the prostaglandin E receptor subtype EP3.2003

    • Author(s)
      K.Yoshida et al.
    • Journal Title

      Eur.J.Neurosci. 18

      Pages: 1848-1860

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysisi of signal transmissions from suprachasmatic nucleus in the rat brain using otical recordings.2003

    • Author(s)
      Hosono, T. et al.
    • Journal Title

      Trans.Jpn.Soc.Med.Biol.Eng. 41

      Pages: 405-409

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of fasting on thermoregulatory processes and their daily oscillations in rats.2003

    • Author(s)
      K.Nagashima, S.Nakai, K.Matsue, M.Tanaka, K.Kanosue
    • Journal Title

      American Journal of Physiology (Reg.Integ.and comp.Physiol) 284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibitory projection from the preoptic area to the medullary raphe for the control of thremoregulatory non-shivering thermogenesis in rats.2003

    • Author(s)
      A.Taniguchi, X.-M.Chen, K.Nagashima, M.Tanaka, K.Kanosue
    • Journal Title

      Brain Research 966

      Pages: 103-109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Systemic salt loading suppresses heat production and thermoregulatory behaviour in rats during cold exposure2003

    • Author(s)
      M.Konishi, K.Nagashima, K.Asano, K.Kanosue
    • Journal Title

      J.Physiol. 551

      Pages: 713-720

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Brain regions expressing Fos by thermoregulatory behavior in rats.2003

    • Author(s)
      M.Maruyama, M.Nishi, Y.Takashige, K.Nagashima, T.Kiyohara, K.Kanosue
    • Journal Title

      American Journal of Physiology Reg.Integ.and comp.Physiol. 285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Neurones of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue express the prostaglandin E receptor subtype EP3.2003

    • Author(s)
      K.Yoshida, T.Huebschle, R.Gerstberger, K.Matsumura, K.Kanosue et al.
    • Journal Title

      Eur.J.Neurosci. 18

      Pages: 1848-1860

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analysisi of signal transmissions from suprachasmatic nucleus in the rat brain using optical recordings.2003

    • Author(s)
      Hosono, T., M.Nakano, R.Terai, T.Yagishita, R.Okuno, K.Akazawa, K.Kanosue
    • Journal Title

      Trans.Jpn.Soc.Med.Biol.Eng. 41

      Pages: 405-409

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Yoshida et al.: "Neurones of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue"Eur.J.Neurosci.. 18. 1848-1860 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Maruyama M et al.: "Brain regions expressing Fos by thermoregulatory behavior in rats."Am.J.Physiol.. 285. R1116-R1123 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Konishi M et al.: "Systemic salt loading suppresses heat production and thermoregulatory behaviour in rats during cold exposure"J.Physiol.. 551. 713-720 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taniguchi A et al.: "Inhibitory projection from the preoptic area to the medullary raph."Brain Research. 966. 103-109 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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