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Identification of membrane proteins participating myogenesis

Research Project

Project/Area Number 15390089
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SEHARA Atsuko  Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (60209038)

Co-Investigator(Kenkyū-buntansha) KURISAKI Tomohiro  Kyoto University, Institute for Frontier Medical Sciences, Assistant, 再生医科学研究所, 助手 (90311422)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2003: ¥7,900,000 (Direct Cost: ¥7,900,000)
KeywordsMyogenesis / cellular differentiation / si RNA / GFP / membrane proteins / 組織形成 / 骨格筋 / 形態形成 / 細胞増殖 / siRNA
Research Abstract

Coordinated development of multi-cellular organisms depends on intercellular communications and adhesions. Our research has been focused on identification of membrane proteins participating such cell-cell interactions during myogenesis. Inititially, we made a cDNA library of mRNA prepared from fusing muscle cells, which was subtracted with mRNA of myoblasts. From the screening of this library, we could identify a couple of novel membrane proteins. We are now trying to know functions of these proteins in myogenesis. We also succeeded to isolate a number of monoclonal antibodies from fusing muscle cells. Finally we studied roles of ADAM proteases in myogenesis, which we had cloned as membrane proteins activated by myogenic transcription factors. Based on the analyses of Meltrin α -/- and Meltrin β -/- mice, we concluded that these ADAM proteins indeed participate in myogenesis in vivo.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2005 2004 2003 Other

All Journal Article (13 results) Publications (3 results)

  • [Journal Article] The acetylcholine receptor γ-to-ε switch occurs in individual endplates.2005

    • Author(s)
      Yumoto, N., et al.
    • Journal Title

      Biochemical and Biophysical Research Communication (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles of Meltrin α (ADAM12) in obesity induced by high-fat diet.2005

    • Author(s)
      Masaki, M., et al.
    • Journal Title

      Endocrinology 146(4)

      Pages: 1752-1763

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The acetylcholine receptor γ-to-ε switch occurs in individual endplates.2005

    • Author(s)
      Yumoto, N.
    • Journal Title

      Biochemical and Biophysical Research Communication (in Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Role of Meltrin α (ADAM12) in obesity induced by high-fat diet.2005

    • Author(s)
      Masaki, M.
    • Journal Title

      Endocrinology 146(4)

      Pages: 1752-1763

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Roles of Meltrin α (ADAM12) in obesity induced by hish-fat diet.2005

    • Author(s)
      Masaki, M., et al.
    • Journal Title

      Endocrinology 146(4)

      Pages: 1752-1763

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A Novel Metalloprotease-Disintegrin, Meltrin ε(ADAM35), Expressed in Epithelial Tissues during Chick Embryogenesis.2004

    • Author(s)
      Watabe-Uchida, M., et al.
    • Journal Title

      Dev.Dynamics 230

      Pages: 557-568

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Lipid rafts identified as locations of ectodomain shedding mediated Meltrin β/ADAM192004

    • Author(s)
      Wakatsuki, S., et al.
    • Journal Title

      J.Neurochemistry 89(1)

      Pages: 119-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Essential Roles of meltrin β/ADAM19 in Heart Development.2004

    • Author(s)
      Kurohara, K., et al.
    • Journal Title

      Developmental Boil. 267

      Pages: 14-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Novel Metalloprotease-Disintegrin, Meltrin ε (ADAM35), Expressed in Epithelial Tissues during Chick Embryogenesis.2004

    • Author(s)
      Watabe-Uchida, M.
    • Journal Title

      Dev.Dynamics 230

      Pages: 557-568

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lipid rafts identified as locations of ectodomain shedding mediated Meltrin β/ADAM192004

    • Author(s)
      Wakatsuki, S.
    • Journal Title

      J.Neurochemistry 89(1)

      Pages: 119-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Essential Roles of Meltrin β/ADAM19 in Heart Development.2004

    • Author(s)
      Kurohara, K.
    • Journal Title

      Developmental Biol. 267

      Pages: 14-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phenotypic analysis of Meltrin alpha (ADAM12)-deficient mice : involvement of Meltrin alpha in adipogenesis and myogenesis2003

    • Author(s)
      Kurisaki, T., et al.
    • Journal Title

      Mol Cell Biol. 23(1)

      Pages: 55-61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phenotypic analysis of Meltrin alpha (ADAM12)-deficient mice : involvement of Meltrin alpha in adipogenesis and myogenesis2003

    • Author(s)
      Kurisaki T.
    • Journal Title

      Mol Cell Biol. 23(1)

      Pages: 55-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Watabe-Uchida M.: "A Novel Metalloprotease-Disintegrin, Meltrin e (ADAM35), Expressed in Epithelial Tissues during Chick Embryogenesis"Dev.Dynamics. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Wakatsuki S.: "Lipid rafts identified as locations of ectodomain shedding mediated Meltrin β/ADAM19"J.Neurochemistry. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Kurohara, K.: "Essential Roles of Meltrinβ / ADAM19 in Heart Development"Developmental Biol.. 267. 14-28 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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