• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of protein complex involved in neuropathic pain by proteomics

Research Project

Project/Area Number 15390109
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKansai Medical University

Principal Investigator

ITO Seiji  Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (80201325)

Co-Investigator(Kenkyū-buntansha) NISHIZAWA Mikio  Kansai Medical University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (40192687)
KINUMI Tomoya  Human Stress Signal Research Center, AIST, Researcher, ヒューマンストレスシグナル研究センター, 研究員 (90293125)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2004: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2003: ¥8,800,000 (Direct Cost: ¥8,800,000)
KeywordsProteomics / 2-Dimenssional gel electrophoresis / MALDI-TOF-MS / Neuropathic pain / Dorsal root ganglia / Postsynaptic density / NMDA receptor / CRMP-2 / 坐骨神経 / 構造蛋白 / TOF-MS / 極性 / 炎症性疼痛 / 一次求心性線維 / 脊髄 / 慢性疼痛 / 神経可塑性
Research Abstract

Since human genome project is accomplished, proteomics that enables global analysis of proteins expressed in cells and tissues has been paid much attention at present. Primary afferent fibers are originated from pseudounipolar sensory neurons in dorsal root ganglia (DRG) and innervate polymodal receptors in the periphery, which are most effectively excited by noxious heat, chemical and mechanical stimuli, and transmit these impulses in the superficial dorsal horn of the spinal cord, an important site of pain processing. Nerve injury often causes intractable and chronic pain. Since neuronal plasticity has been shown to be an important component in the generation of neuropathic pain, changes in gene expression, protein expression and post-translational protein modification have been extensively studied in the DRG as well as the spinal cord. In order to elucidate the mechanisms of generation of neuropathic pain, here we attempted to identify functional molecules involved in 1) neural pola … More rity of axonal transport of proteins produced in the DRG through primary afferent fibers and 2) generation of neuropathic pain, especially NMDA receptor complex in the postsynaptic density (PSD) by use of proteomic technologies and obtained following results.
1)We established large-sized two-dimensional gel electrophoresis (20 X 70 cm) and subsequent identification by MALDI-TOF-MS. We also established differential analysis of protein expression of two samples in a single gel by Ettan DIGE system.
2)We found that 69 and 61 spots were at least 2-fold abundantly expressed in lumbar spinal nerve segments peripheral (P) and central (C) to the DRG respectively, among more than 800 protein spots visualized by silver staining. One of the unique spots in the P fraction was identified as an isoform of collapsin response mediator protein-2 (CRMP-2), which was decreased after nerve injury.
3)We found that phosphorylation of NMDA receptor subtype NR2B increased in the PSD of spinal dorsal horn associated with neuropathic pain. At present, we have examined changes of components of NMDA receptor complex in spinal PSD of inflammatory and neuropathic pain model by proteomics. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (36 results)

All 2005 2004 2003 Other

All Journal Article (25 results) Book (2 results) Patent(Industrial Property Rights) (2 results) Publications (7 results)

  • [Journal Article] Involvement of Rho-kinase in inflammatory and neuropathic pain through phosphorylation of myristoylated alanine-rich C-kinase substrate(MARCKS)2005

    • Author(s)
      Tatsumi, Shinichi
    • Journal Title

      Neuroscience 131

      Pages: 491-498

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of Rho-kinase in inflammatory and neuropathic pain through phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS).2005

    • Author(s)
      Tatsumi S et al.
    • Journal Title

      Neuroscience 131

      Pages: 491-498

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Involvement of Rho-kinase in inflammatory and neuropathic pain through phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS)2005

    • Author(s)
      Tatsumi, Shinichi
    • Journal Title

      Neuroscience 131

      Pages: 491-498

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide is required for the development of spinal sensitization and induction of neuropathic pain2004

    • Author(s)
      Mabuchi, Tamaki
    • Journal Title

      J.Neurosci. 24

      Pages: 7283-7291

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Monitoring for dynamic biological processing by intra-molecular biolumine-scence resonance energy transfer system using secreted luciferase2004

    • Author(s)
      Otsuji, Tomomi
    • Journal Title

      Anal.Biochem. 329

      Pages: 230-237

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide is required for the development of spinal sensitization and induction of neuropathic pain.2004

    • Author(s)
      Mabuchi T et al.
    • Journal Title

      J.Neurosci. 24

      Pages: 7283-7291

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Membrane-associated prostaglandin E synthase-I is required for neuropathic pain.2004

    • Author(s)
      Mabuchi T et al.
    • Journal Title

      Neuroreport 15

      Pages: 1395-1398

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Monitoring for dynamic biological processing by intra-molecular bioluminescence resonance energy transfer system using secreted luciferase.2004

    • Author(s)
      Otsuji T et al.
    • Journal Title

      Anal.Biochem. 329

      Pages: 230-237

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of N-methyl-D-aspartate receptor subunits involved in acute ammonia toxicity.2004

    • Author(s)
      Kitano T et al.
    • Journal Title

      Neurochem.Int. 44

      Pages: 83-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Acute and late effects on induction of allodynia by acromelic acid, a mushroom poison related structurally to kainic acid.2004

    • Author(s)
      Minami T et al.
    • Journal Title

      Br.J.Pharmacol. 142

      Pages: 679-688

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Characterization of N-methyl-D-aspartate receptor subunits responsible for postoperative pain.2004

    • Author(s)
      Nishimura W et al.
    • Journal Title

      Eur.J.Pharmacol. 503

      Pages: 71-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Analgesic effect of extracts of Chinese medicinal herbs Moutan cortex and Coicis serhen on neuropathic pain in mice.2004

    • Author(s)
      Tatsumi S et al.
    • Journal Title

      Neurosci.Lett. 370

      Pages: 130-134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Synthesis of prostaglandin F ethanolamide by prostaglandin F synthase and identification of Bimatoprost as a potent inhibitor of the enzyme : enzyme : new enzyme assay method using LC/ESI/MS.2004

    • Author(s)
      Koda N et al.
    • Journal Title

      Arch.Biochem.Biophys. 424

      Pages: 128-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A simple acromelic acid analogue potentially useful for receptor photoaffinity labeling and biochemical studies.2004

    • Author(s)
      Furuta K et al.
    • Journal Title

      Tetrahedron Lett. 45

      Pages: 3933-3936

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Epidermal growth factor and interleukin-1β synergistically stimulate the production of nitric oxide in rat intestinal epithelial cells.2004

    • Author(s)
      Kitagawa K et al.
    • Journal Title

      Am.J.Physiol. 287

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Monitoring for dynamic biological processing by intra-molecular bioluminescence resonance energy transfer system using secreted luciferase2004

    • Author(s)
      Otsuji, Tomomi
    • Journal Title

      Anal.Biochem. 329

      Pages: 230-237

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Acute and late effects on induction of allodynia by acromelic acid, a mushroom poison related structurally to kainic acid2004

    • Author(s)
      Minami, Toshiaki
    • Journal Title

      Br.J.Pharmacol. 142

      Pages: 679-688

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Membrane-associated prostaglandin E synthase-I is required for neuropathic pain2004

    • Author(s)
      Mabuchi, Tamaki
    • Journal Title

      Neuroreport 15

      Pages: 1395-1398

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synthesis of prostaglandin F ethanolamide by prostaglandin F synthase and identification of Bimatoprost as a potent inhibitor of the enzyme : new enzyme assay method using LC/ESI/MS2004

    • Author(s)
      Koda, Noriko
    • Journal Title

      Arch.Biochem.Biophys. 424

      Pages: 128-136

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional characterization of prostaglandin F_<2α> receptor in the spinal cord for tactile pain(allodynia)2003

    • Author(s)
      Muratani, Tadatoshi
    • Journal Title

      J.Neurochem. 86

      Pages: 374-382

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional evidence for interaction between prostaglandin EP3 and κ-opioid receptor pathways in tactile pain induced by human immunodeficiency virus type-1(HIV-1) glycoprotein gp1202003

    • Author(s)
      Minami, Toshiaki
    • Journal Title

      Neuropharmacology 46

      Pages: 96-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Attenuation of neuropathic pain by the nociceptin/orphanin FQ antagonist JTC-801 is mediated by inhibition of nitric oxide production2003

    • Author(s)
      Mabuchi, Tamaki
    • Journal Title

      Eur.J.Neurosci. 17

      Pages: 1384-1392

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional evidence for interaction between prostaglandin EP3 and κ-opioid receptor pathways in tactile pain induced by human immunodeficiency virus type-1 (HIV-1) glycoprotein gp120.2003

    • Author(s)
      Minami T et al.
    • Journal Title

      Neurophamacology 45

      Pages: 96-105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional characterization of prostaglandin F2a receptor in the spinal cord for tactile pain (allodynia).2003

    • Author(s)
      Muratani T et al.
    • Journal Title

      J.Neurochem. 86

      Pages: 374-382

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Attenuation of neuropathic pain by the nociceptin/orphanin FQ antagonist JTC-801 is mediated by inhibition of nitric oxide production.2003

    • Author(s)
      Mabuchi T et al.
    • Journal Title

      Eur.J.Neurosci. 17

      Pages: 1384-1392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 痛みの基礎と臨床(緒方, 宣邦, 柿木 隆介編)2003

    • Author(s)
      伊藤 誠二(分担)
    • Publisher
      真興貿易(株)医書出版部
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 痛みの基礎と臨床(緒方 宣邦、柿木 隆介編)2003

    • Author(s)
      伊藤 誠二(分担)
    • Publisher
      真興貿易(株)医書出版部
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 蛋白質のプロセッシングを測定するためのモニター蛋白質2003

    • Inventor(s)
      伊藤 誠二(他2名)
    • Industrial Property Rights Holder
      科学技術振興財団
    • Filing Date
      2003
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 蛋白質のプロセッシングを測定するためのモニター蛋白質2003

    • Inventor(s)
      伊藤 誠二(他 2名)
    • Industrial Property Rights Holder
      科学技術振興財団
    • Filing Date
      2003
    • Related Report
      2004 Annual Research Report
  • [Publications] Mabuchi, T.: "Attenuation of neuropathic pain by the nociceptin/orphanin FQ antagonist JTC-801 is mediated by inhibition of nitric oxide production."Eur.J.Neurosci.. 17. 1384-1392 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Muratani, T.: "Functional characterization of prostaglandin F_<2α> receptor in the spinal cord for tactile pain (allodynia)."J.Neurochem.. 86. 374-382 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Minami, T.: "Functional evidence for interaction between prostaglandin EP3 and κ-opioid receptor pathways in tactile pain induced by human immunodeficiency virus type-1 (HIV-1) glycoprotein gp120."Neurophamacology. 45. 96-105 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Muratani, T.: "Preemptive analgesia by Zaltoprofen in a mouse model of post-operative pain through blockade of the bradykinin B_2 pathway."J.Pharmacol.Exp.Ther.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koda, N.: "Synthesis of prostaglandin F ethanolamide by prostaglandin F synthase and identification of bimatoprost as a potent inhibitor of the enzyme-new enzyme assay method by LC/ESI/MS."Arch.Biochem.Biophys.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Otsuji, T.: "Monitoring for dynamic biological processing by intra-molecular bioluminescence resonance energy transfer system using secreted luciferase."Anal.Biochem.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊藤 誠二: "痛み-基礎・診断・治療-"朝倉書店. 407 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi