• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A Role of somatic stem cells in pathogenesis and treatments of hereditary hepatic disorders.

Research Project

Project/Area Number 15390113
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionKumamoto University

Principal Investigator

ENDO Fumio  Kumamoto University, School of Medicine Department of Pediatrics, Professor, 大学院・医学薬学研究部, 教授 (00176801)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Tetsuro  Kumamoto University, School of Medicine Department of Pediatrics, Professor, 大学院・医学薬学研究部, 教授 (60112405)
ADACHI Naoto  Kumamoto University, School of Medicine Department of Hospital, Lecturer, 大学院・医学薬学研究部, 助教授 (00264292)
NAKAMURA Kimitoshi  Kumamoto University, School of Medicine Department of Hospital, Assistant, 医学部附属病院, 助手 (30336234)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2003: ¥9,500,000 (Direct Cost: ¥9,500,000)
Keywordsliver failure / amino acid metabolism / enzyme defect / cancer / mouse model / apoptosis / 遺伝性疾患
Research Abstract

Researches on pathogenesis and quest for new treatments require approaches from vew view points. For treatment of liver disease, employment of stam cell systems are believed to be hopeful. Induction of endodermal cells such as liver cells or pancreatic cells, from ES cells or somatic stem cells.
(1)In the present study, we extended previous investigations on the gene expression during the process of cell injury.
(2)Hereditary tyrosinemia type 1, a genetic disease caused by a deficiency of fumarylacetoacetate hydrolase(FAH), induces severe visceral injuries. Mice with FAH deficiency are lethal after birth ; thus, efforts to elucidate the mechanisms of the disease process have been impeded. The use of Fah(-/-) Hpd(-/-) double-mutant mice has enabled studies on tyrosinemias, and essential features of visceral injury have been revealed. In this mice models, we transplanted endodermal somatic stem cells isolated from salivary glands. Two weeks after, the livers Were dissected and analysed for repopulation. The results indicated 20-40% of hepatic cells are replaced by transplqabted cells.
The results presented here suggested that endodermal stem cells will represent the endodermal stem cells of adults.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

Research Products

(20 results)

All 2004 2003 Other

All Journal Article (16 results) Publications (4 results)

  • [Journal Article] Flow cytometric Isolation of endodermal progenitors from mouse salivary gland differentiate into hepatic and pancreatic lineages.2004

    • Author(s)
      Hisatomi Y., Endo F., et al.
    • Journal Title

      Hepatology 39

      Pages: 667-675

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serum cytocheome C level as a prognostic indicator in patients with systemic inflammatory response syndrome.2004

    • Author(s)
      Adachi N., Endo F., et al.
    • Journal Title

      Clinica Chimica Acta 342

      Pages: 127-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Clinical manifestations of inborn errors of the urea cycle and related metaboli c disorders furing childhood.2004

    • Author(s)
      Endo F., et al.
    • Journal Title

      Am.Soc.Nutr.Sci.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Flow cytometric Isolation of endodermal progenitors from mouse salivary gland differentiate into hepatic and pancreatic lineages.2004

    • Author(s)
      Hisatomi Y., Okumura K., Nakamura K., Matsumoto S., Satoh A., Nagano K., Yamamoto T., Endo F.
    • Journal Title

      Hepatology 39

      Pages: 667-675

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Serum cytocheome C level as a prognostic indicator in patients with systemic inflammatory response syndrome.2004

    • Author(s)
      Adachi N., Hirota M., Hamaguchi M., Okamoto K., Watanabe K., Endo F.
    • Journal Title

      Clinica Chimica Acta 342

      Pages: 127-136

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of systemic inflammatory responses in neonates with herpes simplex virus infection.2004

    • Author(s)
      Kawabe J., Kimura H., Ito Y., Ando Y., Tanaka-Kitajima N., Hayakawa M., Nunoi H., Endo F., Motishima T.
    • Journal Title

      J.Infectious Dis. 190

      Pages: 494-498

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Clinical manifestations of inborn errors of the urea cycle and related metaboli c disorders furing childhood.2004

    • Author(s)
      Endo F., Matsuura T., Yanagita K., Matsuda I.
    • Journal Title

      Am.Soc.Nutr.Sci.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Evaluation of systemic inflammatory responses in neonates with herpes simplex virus infection.2004

    • Author(s)
      Kawabe J., Endo F., et al.
    • Journal Title

      J.Infectious Dis. 190

      Pages: 494-498

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Animal models for tyrosinemias reveal pathophysiologies of tyrosinemias.2003

    • Author(s)
      Endo F., et al.
    • Journal Title

      J Nutrition 133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Salivary Gland Progenitor Cells Induced by Duct Ligation Differentiate into Hepatic and Pancreatic Lineages.2003

    • Author(s)
      Okumura K., Endo F., et al.
    • Journal Title

      Hepatology 38

      Pages: 105-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete absence of bile and pancreatic ducts in a newborn : a new entity of congenital anomaly in hepato-pancreatic development.2003

    • Author(s)
      Nakamura K, Endo F et al.
    • Journal Title

      J Human Genetics 48

      Pages: 380-384

    • NAID

      10011595339

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Animal model reveal pathophysiologies of tyrosinemias.2003

    • Author(s)
      Endo F., Tanaka Y., Tomoeda K., Tanoue A., Tsujimoto G., Nakamura K.
    • Journal Title

      The Journal of Nutrition. 133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages.2003

    • Author(s)
      Okumura K., Nakamura K., Hisatomi Y., Nagano K., Tanaka Y., Terada K., Sugiyama T., Umeyama K., Matsumoto K., Yamamoto T., Endo F.
    • Journal Title

      hepatology 38

      Pages: 105-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Complete absence of bile and pancreatic ducts in a newborn : a new entity of congenital anomaly in hepato-pancreatic development.2003

    • Author(s)
      Nakamura K., Mitsubuchi H., Miyayama H., Yatsunami K., Ishimatsu J., Yamamoto T., Endo F.
    • Journal Title

      J Human Genetics 48

      Pages: 380-384

    • NAID

      10011595339

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A missense mutation(GGC[^<435>Gly→AGC[Ser]] in exon 8 of the CYP11B2 gene inherited in Japanese Patients with Congenital hypoaldosteronism.2003

    • Author(s)
      Kuribayashi I., Kuge H., Santa J.R., Mutlaq Z.A., Yamasaki N., Furuno T., Takahashi A., Chida S., Nakamura T., Endo F., Doi Y., Onishi S., Shizuta Y.
    • Journal Title

      Horm.Res 60

      Pages: 255-260

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] In situ evaluation of podochin n normal and glomerular diseases.2003

    • Author(s)
      Horinouchi I., Nakazato H., Kawano T., Iyama K., Furuse A., Arizono K., Machida J., Sakamoto T., Endo F., Hattori S.
    • Journal Title

      Kidney Int. 64

      Pages: 2092-2099

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Endo F, et al.: "Animal models for tyrosinemias reveal pathophysiologies of tyrosinemias."J Nutrition. 133. 2063S-2067S (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] K Okumura, F Endo et al.: "Salivary Gland Progenitor Cells Induced by Duct Ligation Differentiate into Hepatic and Pancreatic Lineages."Hepatology. 38. 105-113 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura K, Endo F et al.: "Complete absence of bile and pancreatic ducts in a newborn : a new entity of congenital anomaly in hepato-pancreatic development."J Human Genetics. 48. 380-384 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hisatomi Y., Endo F., et al.: "Flow cytometric isolation of endodermal progenitors from mouse salivary gland differentiate into hepatic and pancreatic lineages."Hepatology. 39. 667-675 (2004)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi