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Elucidation of the molecular pathogenesis of dialysis-related amyloidosis.

Research Project

Project/Area Number 15390123
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionUniversity of Fukui (2004-2005)
福井医科大学 (2003)

Principal Investigator

NAIKI Hironobu  University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (10227704)

Co-Investigator(Kenkyū-buntansha) HASEGAWA Kazuhiro  University of Fukui, Faculty of Medical Sciences, Research Associate, 医学部, 助手 (60324159)
HIGUCHI Keiichi  Shinshu University, Granduate School of Medicine, Professor, 大学院・医学研究科, 教授 (20173156)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥8,200,000 (Direct Cost: ¥8,200,000)
KeywordsAmyloid fibril / Dialysis-related amyloidosis / Beta2-microglobulin / Trifluoroethanol / Glycosamonoglycans / Sodium dodecyl sulfate / Lysophospholipids / Transgenic mouse / 分子間相互作用 / リゾフォスファチジン酸 / 陰性荷電 / アミロイド線維形成反応 / 変性中間体 / 透析アミロイドーシス / 界面活性剤
Research Abstract

β2-Microglobulin (β2-m)-related amyloidosis (dialysis-related amyloidosis, DRA) is a frequent and serious complication in patients on long-term dialysis, and β2-m is a major structural component of β2-m amyloid fibril (fAβ2M). The aims of this study are (1) establishment of molecular model of fAβ2M formation from β2-m monomer, (2) elucidation of the effect of various biological molecules or chemical compounds on the fibril formation and degradation, and on the pathogenesis of DRA, (3) proposition of a strategy for the treatment of DRA on the basis of molecular interaction investigated. For these purpose, we improved the previous in vitro amyloid fibril formation system and also developed a transgenic mouse for the model of DRA.
Results : In fAβ2M formation, partial unfolding of β2-m is believed to be prerequisite to its assembly into fibrils. (1) Trifluoroethanol (5-20%) was found to induce the amyloidogenic unfolding of β2-m and to stabilize the fibrils, resulted in the extension of fAβ2M at a neutral pH. In addition, some glycosamonoglycans enhanced the fibril extension probably by binding to the fibrils and stabilizing them. (2) At around the critical micelle concentration, sodium dodecyl sulfate, an analogue of phospholipids, was also found to induce the extension of fAβ2M by inducing the amyloidogenic unfolding of β2-m at a neutral pH. (3) Some anionic lysophospholipids were found to extend fAβ2M at a neutral pH in vitro. These are the first group of physiological agents, which may participate in the fibrillogenesis in the patients. (4) The human β2-m transgenic mice were successfully produced and confirmed that the levels of β2-m in the serum were elevated to several times higher than those of the patients on long-term dialysis.
As the conclusion of this study, the remarkable advance on the clarification of the molecular mechanism of fAβ2M formation in vitro, and on the production of transgenic mouse as a in vivo model system were obtained.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (109 results)

All 2006 2005 2004 2003 Other

All Journal Article (97 results) Publications (12 results)

  • [Journal Article] Anti-Parkinsonian agents have anti-amyloidogenic activity for Alzheimer's β-amyloid fibrils in vitro.2006

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Neurochem. Int. 48(4)

      Pages: 275-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transmission of amyloidosis in offspring of mice with AApoAII amyloidosis.2006

    • Author(s)
      Korenaga, T. et al.
    • Journal Title

      Am. J. Pathol. 168(3)

      Pages: 898-906

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anti-Parkinsonian agents have anti-amyloidogenic activity for Alzheimer's β-amyloid fibrils in vitro.2006

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Neurochem.Int. 48(4)

      Pages: 275-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Transmission of amyloidosis in offspring of mice with AApoAII amyloidosis.2006

    • Author(s)
      Korenaga, T. et al.
    • Journal Title

      Am.J.Pathol. 168(3)

      Pages: 898-906

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transmission of amyloidosis in offspring of mice with AApoAII amyloidosis.2006

    • Author(s)
      Korenaga, T.et al.
    • Journal Title

      Am.J.Pathol. 168(3)

      Pages: 898-906

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Seeding-dependent maturation of β_2-microglobulin amyloid fibrils at neutral pH.2005

    • Author(s)
      Kihara, M. et al.
    • Journal Title

      J. Biol. Chem. 280(12)

      Pages: 12012-12018

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association of thin filaments into thick filaments revealing the structural hierarchy of amyloid fibrils.2005

    • Author(s)
      Kanno, T. et al.
    • Journal Title

      J. Struct. Biol. 149(2)

      Pages: 213-218

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Critical balance of electrostatic and hydrophobic interactions is required for β_2-microglobulin amyloid fibril growth and stability.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      Biochemistry 44(4)

      Pages: 1288-1299

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Metal ion-dependent effects of clioquinol on the fibril growth of an amyloid β peptide.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      J. Biol. Chem. 280(16)

      Pages: 16157-16162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stereospecific amyloid-like fibril formation by a peptide fragment of β_2-microglobulin.2005

    • Author(s)
      Wadai, H. et al.
    • Journal Title

      Biochemistry 44(1)

      Pages: 157-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Molecular interactions in the formation and deposition of β_2-microglobulin-related amyloid fibrils.2005

    • Author(s)
      Naiki, H. et al.
    • Journal Title

      Amyloid 12(1)

      Pages: 15-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Preformed β-amyloid fibrils are destabilized by coenzyme Q10 in vitro.2005

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 330(1)

      Pages: 111-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nuclear magnetic resonance characterization of the refolding intermediate of β_2-microglobulin trapped by non-native prolyl peptide bond.2005

    • Author(s)
      Kameda, A. et al.
    • Journal Title

      J. Mol. Biol. 348(2)

      Pages: 383-397

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural model of the amyloid fibril formed by β_2-microglobulin #21-31 fragment based on vibrational spectroscopy.2005

    • Author(s)
      Hiramatsu, H. et al.
    • Journal Title

      J. Am. Chem. Soc. 127(22)

      Pages: 7988-7989

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ultrasonication-induced amyloid fibril formation of β_2-microglobulin.2005

    • Author(s)
      Ohhashi, Y. et al.
    • Journal Title

      J. Biol. Chem. 280(38)

      Pages: 32843-32848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetically controlled thermal response of β_2-microglobulin amyloid fibrils.2005

    • Author(s)
      Sasahara, K. et al.
    • Journal Title

      J. Mol. Biol. 352(3)

      Pages: 700-711

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Main-chain dominated amyloid structures demonstrated by the effect of high pressure.2005

    • Author(s)
      Chatani, E. et al.
    • Journal Title

      J. Mol. Biol. 352(4)

      Pages: 941-951

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seeding-dependent propagation and maturation of amyloid fibril conformation.2005

    • Author(s)
      Yamaguchi, KI. et al.
    • Journal Title

      J. Mol. Biol. 352(4)

      Pages: 952-960

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cerebrospinal fluid of Alzheimer patients promotes β-amyloid fibril formation in vitro.2005

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Neurobiol. Dis. 20(2)

      Pages: 233-240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetic analysis of the polymerization and depolymerization of β_2-microglobulin-related amyloid fibrils in vitro.2005

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      Biochim. Biophys. Acta. 1753(1)

      Pages: 34-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural studies reveal that the diverse morphology of β_2-microglobulin aggregates is a reflection of different molecular architectures.2005

    • Author(s)
      Kardos, J. et al.
    • Journal Title

      Biochim. Biophys. Acta. 1753(1)

      Pages: 108-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Non-steroidal anti-inflammatory drugs have anti-amyloidogenic effects for Alzheimer's β-amyloid fibrils in vitro.2005

    • Author(s)
      Hirohata, M. et al.
    • Journal Title

      Neuropharmacology 49(7)

      Pages: 1088-1099

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] αB-crystallin, a small heat shock protein, prevents the amyloid fibril growth of an amyloid β-peptide and β2-microglobulin.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      Biochem. J. 392(Pt3)

      Pages: 573-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seeding-dependent maturation of β_2-microglobulin amyloid fibrils at neutral pH.2005

    • Author(s)
      Kihara, M. et al.
    • Journal Title

      J.Biol.Chem. 280(12)

      Pages: 12012-12018

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association of thin filaments into thick filaments revealing the structural hierarchy of amyloid fibrils.2005

    • Author(s)
      Kanno, T. et al.
    • Journal Title

      J.Struct.Biol. 149(2)

      Pages: 213-218

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Metal ion-dependent effects of clioquinol on the fibril growth of an amyloid β peptide.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      J.Biol.Chem. 280(16)

      Pages: 16157-16162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preformed β-amyloid fibrils are destabilized by coenzyme Q10 in vitro.2005

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 330(1)

      Pages: 111-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nuclear magnetic resonance characterization of the refolding intermediate of β_2-microglobulin trapped by non-native prolyl peptide bond.2005

    • Author(s)
      Kameda, A. et al.
    • Journal Title

      J.Mol.Biol. 348(2)

      Pages: 383-397

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural model of the amyloid fibril formed by β_2-microglobulin #21-31 fragment based on vibrational spectroscopy.2005

    • Author(s)
      Hiramatsu, H. et al.
    • Journal Title

      J.Am.Chem.Soc. 127(22)

      Pages: 7988-7989

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ultrasonication-induced amyloid fibril formation of β_2-microglobulin.2005

    • Author(s)
      Ohhashi, Y. et al.
    • Journal Title

      J.Biol.Chem. 280(38)

      Pages: 32843-32848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinetically controlled thermal response of β_2-microglobulin amyloid fibrils.2005

    • Author(s)
      Sasahara, K. et al.
    • Journal Title

      J.Mol.Biol. 352(3)

      Pages: 700-711

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Main-chain dominated amyloid structures demonstrated by the effect of high pressure.2005

    • Author(s)
      Chatani, E. et al.
    • Journal Title

      J.Mol.Biol. 352(4)

      Pages: 941-951

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seeding-dependent propagation and maturation of amyloid fibril conformation.2005

    • Author(s)
      Yamaguchi, KI. et al.
    • Journal Title

      J.Mol.Biol. 352(4)

      Pages: 952-960

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cerebrospinal fluid of Alzheimer patients promotes β-amyloid fibril formation in vitro.2005

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Neurobiol.Dis. 20(2)

      Pages: 233-240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Kinetic analysis of the polymerization and depolymerization of β_2-microglobulin-related amyloid fibrils in vitro.2005

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      Biochim.Biophys.Acta. 1753(1)

      Pages: 34-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Structural studies reveal that the diverse morphology of β_2-microglobulin aggregates is a reflection of different molecular architectures.2005

    • Author(s)
      Kardos, J. et al.
    • Journal Title

      Biochim.Biophys.Acta. 1753(1)

      Pages: 108-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] αB-crystallin, a small heat shock protein, prevents the amyloid fibril growth of an amyloid β-peptide and β2-microglobulin.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      Biochem.J. 392(Pt3)

      Pages: 573-581

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preformed β-amyloid fibrils are destabilized by coenzyme Q_<10> in vitro.2005

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 330(1)

      Pages: 111-116

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nuclear magnetic resonance characterization of the refolding intermediate of β_2-microglobulin trapped by non-native prolyl peptide bond.2005

    • Author(s)
      Kameda, A.et al.
    • Journal Title

      J.Mol.Biol. 348(2)

      Pages: 383-397

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structural model of the amyloid fibril formed by β_2-microglobulin #21-31 fragment based on vibrational spectroscopy.2005

    • Author(s)
      Hiramatsu, H.et al.
    • Journal Title

      J.Am.Chem.Soc. 127(22)

      Pages: 7988-7989

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Ultrasonication-induced amyloid fibril formation of β_2-microglobulin.2005

    • Author(s)
      Ohhashi, Y.et al.
    • Journal Title

      J.Biol.Chem. 280(38)

      Pages: 32843-32848

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Kinetically controlled thermal response of β_2-microglobulin amyloid fibrils.2005

    • Author(s)
      Sasahara, K.et al.
    • Journal Title

      J.Mol.Biol. 352(3)

      Pages: 700-711

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Main-chain dominated amyloid structures demonstrated by the effect of high pressure.2005

    • Author(s)
      Chatani, E.et al.
    • Journal Title

      J.Mol.Biol. 352(4)

      Pages: 941-951

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Seeding-dependent propagation and maturation of amyloid fibril conformation.2005

    • Author(s)
      Yamaguchi, KI.et al.
    • Journal Title

      J.Mol.Biol. 352(4)

      Pages: 952-960

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Kinetic analysis of the polymerization and depolymerization of β_2-microglobulin-related amyloid fibrils in vitro.2005

    • Author(s)
      Yamamoto, S.et al.
    • Journal Title

      Biochim.Biophys.Acta. 1753(1)

      Pages: 34-43

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structural studies reveal that the diverse morphology of β_2-microglobulin aggregates is a reflection of different molecular architectures.2005

    • Author(s)
      Kardos, J.et al.
    • Journal Title

      Biochim.Biophys.Acta. 1753(1)

      Pages: 108-120

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Non-steroidal anti-inflammatory drugs have anti-amyloidogenic effects for Alzheimer's β-amyloid fibrils in vitro.2005

    • Author(s)
      Hirohata, M.et al.
    • Journal Title

      Neuropharmacology 49(7)

      Pages: 1088-1099

    • Related Report
      2005 Annual Research Report
  • [Journal Article] αB-crystallin, a small heat-shock protein, prevents the amyloid fibril growth of an amyloid β-peptide and β2-microglobulin.2005

    • Author(s)
      Raman, B.et al.
    • Journal Title

      Biochem.J. 392(Pt3)

      Pages: 573-581

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Seeding-dependent maturation of beta 2-microglobulin amyloid fibrils at neutral pH.2005

    • Author(s)
      Kihara, M. et al.
    • Journal Title

      J.Biol.Chem. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Critical Balance of Electrostatic and Hydrophobic Interactions Is Required for beta2-Microglobulin Amyloid Fibril Growth and Stability.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      Biochemistry 44(4)

      Pages: 1288-1299

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Metal ion-dependent effects of clioquinol on the fibril growth of an amyloid beta peptide.2005

    • Author(s)
      Raman, B. et al.
    • Journal Title

      J.Biol.Chem. in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Glycosaminoglycans enhance the trifluoroethanol-induced extension of β_2-microglobulin-related amyloid fibrils at a neutral pH.2004

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      J. Am. Soc. Nephrol. 15(1)

      Pages: 126-133

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimum amyloid fibril formation of a peptide fragment suggests the amyloidogenic preference of β_2-microglobulin under physiological conditions.2004

    • Author(s)
      Ohhashi, Y. et al.
    • Journal Title

      J. Biol. Chem. 279(11)

      Pages: 10814-10821

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Increase in the conformational flexibility of β2-microglobulin upon copper binding : A possible role for copper in dialysis-related amyloidosis.2004

    • Author(s)
      Villanueva, J. et al.
    • Journal Title

      Protein Sci. 13(3)

      Pages: 797-809

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Core and heterogeneity of β_2-microglobulin amyloid fibrils as revealed by H/D exchange.2004

    • Author(s)
      Yamaguchi, K. et al.
    • Journal Title

      J. Mol. Biol. 338(3)

      Pages: 559-571

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Curcumin has potent anti-amyloidogenic effects for Alzheimer's β-amyloid fibrils in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      J. Neurosci. Res. 75(6)

      Pages: 742-750

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Core structure of amyloid fibril proposed from IR-microscope linear dichroism.2004

    • Author(s)
      Hiramatsu, H. et al.
    • Journal Title

      J. Am. Chem. Soc. 126(10)

      Pages: 3008-3009

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of AApoAII amyloidosis by various heterogeneous amyloid fibrils.2004

    • Author(s)
      Fu, X.et al.
    • Journal Title

      FEBS Lett. 563(1-3)

      Pages: 179-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Tissue distribution, biochemical properties, and transmission of mouse type A AApoAII amyloid fibrils.2004

    • Author(s)
      Korenaga, T. et al.
    • Journal Title

      Am. J. Pathol. 164(5)

      Pages: 1597-1606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A seed for Alzheimer amyloid in the brain.2004

    • Author(s)
      Hayashi, H. et al.
    • Journal Title

      J. Neurosci. 24(20)

      Pages: 4894-4902

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Environment-and mutation-dependent aggregation behavior of Alzheimer amyloid β-protein.2004

    • Author(s)
      Yamamoto, N. et al.
    • Journal Title

      J. Neurochem. 90(1)

      Pages: 62-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reactivation of HSV-1 in the brain of patients with familial Alzheimer's disease.2004

    • Author(s)
      Mori, I. et al.
    • Journal Title

      J. Med. Virol. 73(4)

      Pages: 605-611

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Low concentrations of sodium dodecyl sulfate induce the extension of β_2-microglobulin-related amyloid fibrils at a neutral pH.2004

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      Biochemistry 43(34)

      Pages: 11075-11082

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct measurement of the thermodynamic parameters of amyloid formation by isothermal titration calorimetry.2004

    • Author(s)
      Kardos, J. et al.
    • Journal Title

      J. Biol. Chem. 279(53)

      Pages: 55308-55314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vitamin A exhibits potent antiamyloidogenic and fibril-destabilizing effects in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Exp. Neurol. 189(2)

      Pages: 380-392

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conformational stability of amyloid fibrils of β_2-microglobulin probed by guanidine-hydrochloride-induced unfolding.2004

    • Author(s)
      Narimoto, T. et al.
    • Journal Title

      FEBS Lett. 576(3)

      Pages: 313-319

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Anti-amyloidogenic activity of tannic acid and its activity to destabilize Alzheimer's β-amyloid fibrils in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochim. Biophys. Acta. -Molecular Basis of Disease 1690(3)

      Pages: 193-202

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct observation of Aβ amyloid fibril growth and inhibition.2004

    • Author(s)
      Ban, T.et al.
    • Journal Title

      J. Mol. Biol. 344(3)

      Pages: 757-767

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycosaminoglycans enhance the trifluoroethanol-induced extension of β_2-microglobulin-related amyloid fibrils at a neutral pH.2004

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      J.Am.Soc.Nephrol. 15(1)

      Pages: 126-133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimum amyloid fibril formation of a peptide fragment suggests the amyloidogenic preference of β_2-microglobulin under physiological conditions.2004

    • Author(s)
      Ohhashi, Y, et al.
    • Journal Title

      J.Biol.Chem. 279(11)

      Pages: 10814-10821

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Core and heterogeneity of β_2-microglobulin amyloid fibrils as revealed by H/D exchange.2004

    • Author(s)
      Yamaguchi, K. et al.
    • Journal Title

      J.Mol.Biol. 338(3)

      Pages: 559-571

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Curcumin has potent anti-amyloidogenic effects for Alzheimer's β-amyloid fibrils in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      J.Neurosci.Res. 75(6)

      Pages: 742-750

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Core structure of amyloid fibril proposed from IR-microscope linear dichroism.2004

    • Author(s)
      Hiramatsu, H. et al.
    • Journal Title

      J.Am.Chem.Soc. 126(10)

      Pages: 3008-3009

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Tissue distribution, biochemical properties, and transmission of mouse type AAApoAII amyloid fibrils.2004

    • Author(s)
      Korenaga, T. et al.
    • Journal Title

      Am.J.Pathol. 164(5)

      Pages: 1597-1606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A seed for Alzheimer amyloid in the brain.2004

    • Author(s)
      Hayashi, H. et al.
    • Journal Title

      J.Neurosci. 24(20)

      Pages: 4894-4902

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Environment- and mutation-dependent aggregation behavior of Alzheimer amyloid β-protein.2004

    • Author(s)
      Yamamoto, N. et al.
    • Journal Title

      J.Neurochem. 90(1)

      Pages: 62-69

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reactivation of HSV-1 in the brain of patients with familial Alzheimer's disease.2004

    • Author(s)
      Mori, I. et al.
    • Journal Title

      J.Med.Virol. 73(4)

      Pages: 605-611

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Direct measurement of the thermodynamic parameters of amyloid formation by isothermal titration calorimetry.2004

    • Author(s)
      Kardos, J. et al.
    • Journal Title

      J.Biol.Chem. 279(53)

      Pages: 55308-55314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Vitamin A exhibits potent antiamyloidogenic and fibril-destabilizing effects in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Exp.Neurol. 189(2)

      Pages: 380-392

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Anti-amyloidogenic activity of tannic acid and its activity to destabilize Alzheimer's β-amyloid fibrils in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochim.Biophys.Acta. -Molecular Basis of Disease 1690(3)

      Pages: 193-202

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Direct observation of Aβ amyloid fibril growth and inhibition.2004

    • Author(s)
      Ban, T.et al.
    • Journal Title

      J.Mol.Biol. 344(3)

      Pages: 757-767

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tissue distribution, biochemical properties, and transmission of mouse type A AApoAII amyloid fibrils.2004

    • Author(s)
      Korenaga, T. et al.
    • Journal Title

      Am.J.Pathol. 164(5)

      Pages: 1597-1606

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Environment- and mutation-dependent aggregation behavior of Alzheimer amyloid beta-protein.2004

    • Author(s)
      Yamamoto, N. et al.
    • Journal Title

      J.Neurochem. 90(1)

      Pages: 62-69

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Low concentrations of sodium dodecyl sulfate induce the extension of beta 2-microglobulin-related amyloid fibrils at a neutral pH.2004

    • Author(s)
      Yamamoto, S. et al.
    • Journal Title

      Biochemistry 43(34)

      Pages: 11075-11082

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Conformational stability of amyloid fibrils of beta2-microglobulin probed by guanidine-hydrochloride-induced unforlding.2004

    • Author(s)
      Narimoto, T. et al.
    • Journal Title

      FEBS Lett. 576(3)

      Pages: 313-319

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anti-amyloidogenic activity of tannic acid and its activity to destabilize Alzheimer's beta-amyloid fibrils in vitro.2004

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Biochim Biophys Acta. 1690(3)

      Pages: 193-202

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Direct observation of Abeta amyloid fibril growth and inhibition.2004

    • Author(s)
      Ban, T.et al.
    • Journal Title

      J.Mol.Biol. 344(3)

      Pages: 757-767

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Amyloidogenic synthetic peptides of β2-microglobulin : a role of the disulfide bond.2003

    • Author(s)
      Hasegawa, K. et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 304(1)

      Pages: 101-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glycosaminoglycan and proteoglycan inhibit the depolymerization of β2-microglobulin amyloid fibrils in vitro.2003

    • Author(s)
      Yamaguchi, I. et al.
    • Journal Title

      Kidney Int. 64(3)

      Pages: 1080-1088

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Potent anti-amyloidogenic and fibril-destabilizing effects of polyphenols in vitro : Implications for the prevention and therapeutics of Alzheimer's disease.2003

    • Author(s)
      Ono, K.et al.
    • Journal Title

      Neurochem. 87(1)

      Pages: 172-181

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conformational dynamics of β2-microglobulin analyzed by reduction and reoxidation of the disulfide bond.2003

    • Author(s)
      Gozu, M. et al.
    • Journal Title

      J. Biochem. (Tokyo). 133(6)

      Pages: 731-736

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloid fibril formation in the context of full-length protein : effects of proline mutations on the amyloid fibril formation of β2-microglobulin.2003

    • Author(s)
      Chiba, T. et al.
    • Journal Title

      J. Biol. Chem. 278(47)

      Pages: 47016-47024

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dissolution of β2-microglobulin amyloid fibrils by dimethylsulfoxide.2003

    • Author(s)
      Hirota-Nakaoka, N. et al.
    • Journal Title

      J. Biochem. (Tokyo). 134(1)

      Pages: 159-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloidogenic synthetic peptides of β2-microglobulin : a role of the disulfide bond.2003

    • Author(s)
      Hasegawa, K. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 304(1)

      Pages: 101-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Conformational dynamics of β2-microglobulin analyzed by reduction and reoxidation of the disulfide bond.2003

    • Author(s)
      Gozu, M. et al.
    • Journal Title

      J.Biochem. (Tokyo) 133(6)

      Pages: 731-736

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amyloid fibril formation in the context of full-length protein : effects of proline mutations on the amyloid fibril formation of β2-microglobulin.2003

    • Author(s)
      Chiba, T. et al.
    • Journal Title

      J.Biol.Chem. 278(47)

      Pages: 47016-47024

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dissolution of β2-microglobulin amyloid fibrils by dimethylsulfoxide.2003

    • Author(s)
      Hirota-Nakaoka, N. et al.
    • Journal Title

      J.Biochem. (Tokyo) 134(1)

      Pages: 159-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Ban, T., et al.: "Direct observation of amyloid fibril growth monitored by thioflavin T fluorescence"J.Biol.Chem.. 278(19). 16462-16465 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hasegawa, K. et al.: "Amyloidogenic synthetic peptides of β2-microglobulin : a role of the disulfide bond"Biochem.Biophys.Res.Commun.. 304(1). 101-106 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamaguchi, I. et al.: "Glycosaminoglycan and proteoglycan inhibit the depolymerization of β2-microglobulin amyloid fibrils in vitro"Kidney Int.. 64(3). 1080-1088 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ono, K.et al.: "Potent anti-amyloidogenic and fibril-destabilizing effects of polyphenols in vitro : Implications for the prevention_and_therapeutics_of_Alzheimer's_disease"J.Neurochem.. 87(1). 172-181 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Gozu, M. et al.: "Conformational dynamics of β2-microglobulin analyzed by reduction and reoxidation of the disulfide bond"J.Biochem.(Tokyo). 133(6). 731-736 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chiba, T. et al.: "Amyloid fibril formation in the context of full-length protein : effects of proline mutations on the amyloid fibril_formation_of_β2-microglobulin"J.Biol.Chem.. 278(47). 47016-47024 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirota-Nakaoka, N., et al.: "Dissolution of β2-microglobulin amyloid fibrils by dimethylsulfoxide"J.Biochem.(Tokyo). 134(1). 159-164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamamoto, S. et al.: "Glycosaminoglycans enhance the trifluoroethanol-induced extension of β2-microglobulin-related amyloid fibrils at a neutral pH"J.Am.Soc.Nephrol.. 15(1). 126-133 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ohhashi, Y. et al.: "Optimum amyloid fibril formation of a peptide fragment suggests the amyloidogenic preference of beta 2-microglobulin under physiological conditions"J Biol.Chem.. 279(11). 10814-10821 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Villanueva, J. et al.: "Increase in the conformational flexibility of β2-microglobulin upon copper binding : A possible role for copper in dialysis-related amyloidosis"Protein Sci.. 13(3). 797-809 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamaguchi, K. et al.: "Core and heterogeneity of β_2-microglobulin amyloid fibrils as revealed by H/D exchange"J.Mol.Biol.. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ono, K.et al.: "Curcumin has potent anti-amyloidogenic effects for Alzheimer's β-amyloid fibrils in vitro"J.Neurosci.Res.. 75(6). 742-750 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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