| Project/Area Number |
15390133
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
FUJIMOTO Junichiro National Research Institute for Child Health and Development, Vice General Director, 副所長 (60175578)
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| Co-Investigator(Kenkyū-buntansha) |
KIYOKAWA Nobutaka National Research Institute for Child Health and Development, Department of Developmental Biology, Director, 発生・分化研究部, 部長 (60195401)
YAMADA Taketo Keio University, School of Medicine, Department of Pathology, Lecturer, 医学部, 専任講師 (60230463)
YASUE Hiroshi National Institute of Agrobiological Sciences, Genome Research Group, Senior Investigator, ゲノム研究グループ, 上席研究官 (80113497)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Immunodeficient pig / Regenerative medicine / cDNA library / porcine T-cell / MHC / TCR / 腫瘍組織適合抗原 |
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| Research Abstract |
The monoclonal antibody 7G3 against the framework of porcine T-cell receptor gamma chain was established. When compared with previously established other antibody against the same molecule, it was found 7G3 worked better than others in terms of strength of reactivity on cells and tissue sections as well as stability in immunobiochemical analysis. In order to understand the mechanism involved in porcine TCR gene editing, TCR Ja chain was analyzed and it was found that usage of Ja gene segment was very similar to that of human system. In addition, variability of CDR3 region of porcine TCR alpha cDNA was investigated and it was found that average length was 9.35 (average length in human system is 10.2) and that at least 33 porcine TCR alpha genes were identified.
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